FastQCFastQC Report
Fri 10 Feb 2017
ERR1630786.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630786.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951124
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47390.498252593773262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45320.47648887001064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33970.35715637498370345No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG29300.30805657306513134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26730.28103591119559596No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC19090.2007098969219576No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA17840.18756755165467384No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG16280.1711659047611037No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA15490.16285994255218036No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC15480.1627548037900421No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA15370.1615982774065211No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC15330.16117772235796804No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA14860.15623620053746937No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT14670.15423856405684222No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA14010.1472994057557164No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT13850.14561718556150408No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT13710.1441452428915683No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG13630.14330413279446214No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG13470.1416219126002498No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG13200.1387831660225165No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT12690.13342108915346476No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC12500.13142345267283762No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG12210.1283744285708278No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG12060.12679734713875374No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT11900.1251151269445414No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG11600.12196096408039331No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA11400.1198581888376279No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA9960.104718207089717No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG9940.10450792956544046No Hit
TTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATCAG9710.10208973803626026No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA9600.10093321165273929No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG9520.10009210155563313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.018.5948721
TAACGGC609.235217E-418.536
ATCTTAC902.1514206E-618.51
TACCCCG752.0667943E-417.2666665
TTACACT752.0667943E-417.2666664
ATAATAC650.001579663417.0769233
TACTCCG1757.2759576E-1216.9142865
GCCGTCT2200.016.81818236
GTTTACC700.002592052315.85714315
TTAACGG700.002592052315.85714335
TCCGAAA957.057629E-515.57894821
TTACTCC1902.7284841E-1115.5789484
GTTTAAC855.362943E-415.23529433
ACCCCGC855.362943E-415.2352946
ATCGGAA750.00410371414.818
CCGTCTT2550.014.50980537
CGAAAAC1152.20894E-514.47826123
CGCAAGA1152.20894E-514.4782612
TCGTTAA908.2721625E-414.38888922
CTTATAC7000.014.27142837
Produced by FastQC (version 0.11.5)