##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630786.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	951124
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.070477666424146	31.0	30.0	31.0	26.0	34.0
2	30.278393774103062	31.0	30.0	31.0	27.0	34.0
3	30.36320500796952	31.0	30.0	31.0	27.0	34.0
4	34.14367632401243	35.0	33.0	37.0	30.0	37.0
5	32.93239262178223	35.0	32.0	37.0	26.0	37.0
6	33.18941589109306	35.0	32.0	37.0	28.0	37.0
7	33.19144191503947	35.0	32.0	37.0	28.0	37.0
8	33.18432822639319	35.0	32.0	37.0	27.0	37.0
9	34.56797536388525	37.0	33.0	39.0	28.0	39.0
10	34.01114996572476	35.0	32.0	38.0	27.0	39.0
11	34.4275972428411	37.0	33.0	39.0	27.0	39.0
12	34.191657449501854	35.0	32.0	38.0	27.0	39.0
13	34.437384610208554	37.0	33.0	39.0	27.0	39.0
14	35.18339354279779	37.0	33.0	39.0	27.0	40.0
15	35.13889251033514	37.0	33.0	39.0	27.0	40.0
16	35.06920338462703	37.0	33.0	39.0	27.0	40.0
17	34.95652617324345	37.0	33.0	39.0	27.0	40.0
18	34.875621895778046	37.0	33.0	39.0	27.0	40.0
19	34.93053166569238	37.0	33.0	39.0	27.0	40.0
20	34.843569292752576	37.0	33.0	39.0	27.0	40.0
21	34.76659089666542	37.0	33.0	39.0	27.0	40.0
22	34.70303030940235	37.0	33.0	39.0	27.0	40.0
23	34.60058625373768	37.0	32.0	39.0	27.0	40.0
24	34.48842422228858	37.0	32.0	39.0	26.0	40.0
25	34.38476371114597	37.0	32.0	39.0	26.0	40.0
26	34.08056888481418	36.0	32.0	39.0	25.0	40.0
27	33.90202854727669	36.0	32.0	39.0	24.0	40.0
28	33.718165034212156	36.0	31.0	39.0	24.0	40.0
29	33.52420819998234	36.0	31.0	39.0	24.0	40.0
30	33.364287937219544	36.0	31.0	39.0	24.0	40.0
31	33.20043443336515	36.0	31.0	38.0	23.0	40.0
32	33.06461828321018	36.0	30.0	38.0	23.0	40.0
33	32.92361248375606	35.0	30.0	38.0	22.0	40.0
34	32.822074724221025	35.0	30.0	38.0	22.0	40.0
35	32.73842317090096	35.0	30.0	38.0	21.0	40.0
36	32.65256896051409	35.0	30.0	38.0	21.0	40.0
37	32.39978804025553	35.0	30.0	38.0	20.0	40.0
38	32.18533125018399	35.0	30.0	38.0	19.0	40.0
39	32.02952191302081	35.0	30.0	38.0	19.0	40.0
40	31.792906077441007	35.0	29.0	38.0	18.0	40.0
41	31.634783687510776	35.0	29.0	38.0	18.0	40.0
42	31.459046349371903	34.0	29.0	38.0	16.0	40.0
43	30.578833043851276	34.0	27.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	1.0
11	4.0
12	5.0
13	20.0
14	33.0
15	63.0
16	127.0
17	250.0
18	535.0
19	901.0
20	1722.0
21	2957.0
22	4566.0
23	7031.0
24	10288.0
25	14802.0
26	20228.0
27	27117.0
28	35345.0
29	44882.0
30	56000.0
31	68671.0
32	81822.0
33	95737.0
34	107971.0
35	112100.0
36	103710.0
37	80183.0
38	51902.0
39	22146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89240940192867	21.782017907233968	12.193047383937321	23.13252530690005
2	20.556730773274566	23.048834852237984	32.44256269424387	23.951871680243585
3	23.131579058040803	23.80131297286158	28.09507487982639	24.97203308927122
4	15.890672509578142	16.79444530891871	33.17664153149327	34.138240650009884
5	14.963979460091428	37.55157056282882	32.879939944739064	14.604510032340684
6	35.73203914526392	34.34494345637372	15.229980528301251	14.693036870061107
7	29.67804408258019	30.252732556427976	21.097985120762385	18.971238240229454
8	27.423343328524986	33.420248043367636	20.09054550195348	19.065863126153896
9	26.624499013798413	13.006085431552563	20.40459498446049	39.964820570188536
10	17.236553803710137	27.403892657529404	33.216804538630086	22.142749000130372
11	35.70974972769061	22.11593861578511	20.898431750223946	21.275879906300336
12	21.55512845853958	25.547457534453972	29.170329000214483	23.727085006791963
13	30.502962810317058	20.110101311711194	25.777501146012504	23.60943473195924
14	23.034220564300764	21.563960114559194	25.92269777652546	29.479121544614586
15	25.294073117700744	28.138812604875916	23.805939078395667	22.76117519902768
16	26.077987728203684	25.92711360453527	24.759652789751915	23.235245877509136
17	23.58325518018681	26.686636022222128	25.699803600792325	24.030305196798736
18	22.77915392735332	24.628229337079077	28.14617231822559	24.446444417342008
19	25.055618405171142	25.917651115942817	27.516075716730942	21.51065476215509
20	24.551057485669585	25.080746569322194	27.267527683036068	23.100668261972153
21	24.19295486182664	25.50929216379778	26.919623519120535	23.378129455255046
22	25.42244754627157	24.487448534575933	27.09993649618767	22.99016742296483
23	22.699248468128236	25.33938792418234	28.113474163200593	23.84788944448884
24	23.758100941622754	26.005126566041863	26.893759383634517	23.343013108700863
25	24.103271497722695	25.590354149406387	27.237352858302387	23.06902149456853
26	24.61277393904475	25.78927668737199	26.31507563682548	23.28287373675777
27	24.841135330409074	25.194716987480074	25.713997333680993	24.25015034842986
28	23.46907448450465	25.70863525681194	27.61469587561664	23.20759438306677
29	24.402811831054628	26.105534083883907	27.706692292487624	21.78496179257384
30	24.344354679305745	25.438428638116584	26.398030120152576	23.819186562425088
31	22.846863290170365	25.21984515163112	27.826340203800974	24.106951354397534
32	22.7999714022567	26.01069892043519	27.254175060244513	23.9351546170636
33	23.6433945521299	25.212485438281444	27.33008524650834	23.814034763080315
34	24.729793381304646	25.587515402828654	27.16659447138333	22.51609674448337
35	24.66040179829339	25.383020510469716	26.317283550830382	23.63929414040651
36	23.267628616247723	25.628519520062575	26.034775696964857	25.06907616672484
37	22.92687388815759	25.044894251433043	28.435619330392253	23.592612530017114
38	23.194452037799486	24.05616933228475	27.591249931659807	25.15812869825596
39	23.02843793238316	24.84576143594316	28.6211892455663	23.504611386107385
40	23.889208978009176	24.512576698726978	27.70627173743907	23.891942585824772
41	22.4472308552828	25.347483608866984	27.97763488251795	24.227650653332265
42	22.419158805791884	26.0442381855573	27.435118869884473	24.101484138766345
43	23.069336910854947	24.238690223356787	27.304115972260192	25.387856893528078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	303.0
1	273.5
2	244.0
3	525.5
4	807.0
5	807.0
6	1253.5
7	1700.0
8	1817.5
9	1935.0
10	2611.5
11	3288.0
12	3288.0
13	5472.0
14	7656.0
15	10147.5
16	12639.0
17	12189.5
18	11740.0
19	11740.0
20	13125.5
21	14511.0
22	13500.5
23	12490.0
24	13316.5
25	14143.0
26	14143.0
27	15167.0
28	16191.0
29	17841.0
30	19491.0
31	22045.5
32	24600.0
33	24600.0
34	32544.0
35	40488.0
36	42184.5
37	43881.0
38	44292.0
39	44703.0
40	44703.0
41	45381.0
42	46059.0
43	50211.5
44	54364.0
45	53456.0
46	52548.0
47	52548.0
48	53033.0
49	53518.0
50	57358.0
51	61198.0
52	66333.5
53	71469.0
54	71469.0
55	70723.0
56	69977.0
57	63206.5
58	56436.0
59	54265.5
60	52095.0
61	52095.0
62	49009.5
63	45924.0
64	38017.0
65	30110.0
66	26778.5
67	23447.0
68	23447.0
69	22238.0
70	21029.0
71	20680.0
72	20331.0
73	17176.5
74	14022.0
75	14022.0
76	8749.5
77	3477.0
78	2631.0
79	1785.0
80	1460.5
81	1136.0
82	1136.0
83	874.0
84	612.0
85	492.0
86	372.0
87	305.5
88	239.0
89	239.0
90	186.5
91	134.0
92	77.5
93	21.0
94	14.5
95	8.0
96	8.0
97	5.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	951124.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.62147542359507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.58917644727092	51.622605288765435
2	6.229308746688817	7.17883921706631
3	1.510016079449369	2.6102806323367562
4	0.6850777650771708	1.579007664145825
5	0.364822457767202	1.051080414210419
6	0.2631535761141983	0.9097978391217257
7	0.19155062542558976	0.772620075873442
8	0.13168760165659052	0.6070427121957926
9	0.10810809472380431	0.560641313089744
>10	0.7438176203975733	8.465407716194159
>50	0.08703227717898998	3.4961899367623963
>100	0.07615079793747723	9.766790408261867
>500	0.015119528858868154	6.13809633440179
>1k	0.0049783814535297585	5.2416004475743385
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4739	0.498252593773262	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4532	0.47648887001064	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3397	0.35715637498370345	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	2930	0.30805657306513134	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2673	0.28103591119559596	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	1909	0.2007098969219576	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	1784	0.18756755165467384	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	1628	0.1711659047611037	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	1549	0.16285994255218036	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1548	0.1627548037900421	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1537	0.1615982774065211	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	1533	0.16117772235796804	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	1486	0.15623620053746937	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	1467	0.15423856405684222	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1401	0.1472994057557164	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1385	0.14561718556150408	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	1371	0.1441452428915683	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	1363	0.14330413279446214	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1347	0.1416219126002498	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	1320	0.1387831660225165	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	1269	0.13342108915346476	No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC	1250	0.13142345267283762	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	1221	0.1283744285708278	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	1206	0.12679734713875374	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	1190	0.1251151269445414	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1160	0.12196096408039331	No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA	1140	0.1198581888376279	No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA	996	0.104718207089717	No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG	994	0.10450792956544046	No Hit
TTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATCAG	971	0.10208973803626026	No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA	960	0.10093321165273929	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	952	0.10009210155563313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.308325728296206E-4	0.0
3	0.0	0.0	0.0	0.001051387621382701	0.0
4	0.0	0.0	0.0	0.0019976364806271315	0.0
5	0.0	0.0	0.0	0.002313052767041942	0.0
6	0.0	0.0	0.0	0.002313052767041942	0.0
7	0.0	0.0	0.0	0.002418191529180212	0.0
8	0.0	0.0	0.0	0.002523330291318482	1.0513876213827009E-4
9	0.0	0.0	0.0	0.004415828009807343	1.0513876213827009E-4
10	0.0	0.0	0.0	0.006834019538987556	1.0513876213827009E-4
11	0.0	0.0	0.0	0.007464852111817176	1.0513876213827009E-4
12	0.0	0.0	0.0	0.008200823446785067	1.0513876213827009E-4
13	0.0	0.0	0.0	0.008831656019614687	1.0513876213827009E-4
14	0.0	0.0	0.0	0.009988182403135658	1.0513876213827009E-4
15	0.0	0.0	0.0	0.01167040259734798	1.0513876213827009E-4
16	0.0	0.0	0.0	0.013773177840113381	2.1027752427654018E-4
17	0.0	1.0513876213827009E-4	0.0	0.017242756990676296	2.1027752427654018E-4
18	0.0	1.0513876213827009E-4	0.0	0.018609560898473806	2.1027752427654018E-4
19	0.0	1.0513876213827009E-4	0.0	0.020922613665515746	2.1027752427654018E-4
20	0.0	2.1027752427654018E-4	0.0	0.02334080519469596	2.1027752427654018E-4
21	0.0	2.1027752427654018E-4	0.0	0.027966910728779843	2.1027752427654018E-4
22	0.0	2.1027752427654018E-4	0.0	0.03553690160273529	2.1027752427654018E-4
23	0.0	2.1027752427654018E-4	0.0	0.04636619410297711	2.1027752427654018E-4
24	0.0	3.154162864148103E-4	0.0	0.06224214718585589	2.1027752427654018E-4
25	0.0	3.154162864148103E-4	0.0	0.06812991786559902	2.1027752427654018E-4
26	0.0	3.154162864148103E-4	0.0	0.07916948789011738	2.1027752427654018E-4
27	0.0	3.154162864148103E-4	0.0	0.09494030221085789	2.1027752427654018E-4
28	0.0	3.154162864148103E-4	0.0	0.13068748133786973	2.1027752427654018E-4
29	0.0	4.2055504855308037E-4	0.0	0.17652798163015548	2.1027752427654018E-4
30	0.0	4.2055504855308037E-4	0.0	0.23877012881601137	2.1027752427654018E-4
31	0.0	4.2055504855308037E-4	0.0	0.4917339905206892	2.1027752427654018E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1950	0.0	18.594872	1
TAACGGC	60	9.235217E-4	18.5	36
ATCTTAC	90	2.1514206E-6	18.5	1
TACCCCG	75	2.0667943E-4	17.266666	5
TTACACT	75	2.0667943E-4	17.266666	4
ATAATAC	65	0.0015796634	17.076923	3
TACTCCG	175	7.2759576E-12	16.914286	5
GCCGTCT	220	0.0	16.818182	36
GTTTACC	70	0.0025920523	15.857143	15
TTAACGG	70	0.0025920523	15.857143	35
TCCGAAA	95	7.057629E-5	15.578948	21
TTACTCC	190	2.7284841E-11	15.578948	4
GTTTAAC	85	5.362943E-4	15.235294	33
ACCCCGC	85	5.362943E-4	15.235294	6
ATCGGAA	75	0.004103714	14.8	18
CCGTCTT	255	0.0	14.509805	37
CGAAAAC	115	2.20894E-5	14.478261	23
CGCAAGA	115	2.20894E-5	14.478261	2
TCGTTAA	90	8.2721625E-4	14.388889	22
CTTATAC	700	0.0	14.271428	37
>>END_MODULE