FastQCFastQC Report
Fri 10 Feb 2017
ERR1630785.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630785.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388107
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT180480.7557450315249694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT164450.6886207360055475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT137770.5769004487654866No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84370.3532923776028461No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC45170.1891456287343909No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT38970.16318364294397195No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA33190.13898037232000074No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA32350.13546294198710526No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG28980.12135134648489368No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG28880.1209326047785966No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG25920.10853785027220303No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG25750.10782598937149801No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG24310.10179610880082007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT1900.020.4473699
GTACGAC555.1453256E-420.1818183
GACGGTA2100.018.58
GCTTTAT9700.017.164951
CGACGGT2300.016.8913047
TACGACG2300.016.8913045
AGTCGGT5200.016.36538511
CAGTCGG5500.016.14545410
CGCCTTA4050.015.98765525
CTAACGT700.002593590915.8571424
GCAGTCG5550.015.6666659
AATGCGT1302.592169E-715.65384635
CGTTTAG1201.9375293E-615.41666626
TACTCCG3850.015.3766235
TCGGTGA5450.015.27522913
TCACGTA3300.015.13636525
GGTATCA85700.014.9165691
CGTACAC1001.0941477E-414.83
ATCACGT3500.014.79999924
TCCGGTC3800.014.6052648
Produced by FastQC (version 0.11.5)