FastQCFastQC Report
Fri 10 Feb 2017
ERR1630784.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630784.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019625
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86130.8447223243839647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80080.785386784356994No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69320.6798577908544808No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.49498590167953904No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16290.15976461934534755No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13620.13357852151526295No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.120926811327694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG500.007034349318.530
GTCATAA500.007034349318.51
ATTACGT500.007034349318.517
TACGATG500.007034349318.521
AATTACG609.2358416E-418.516
TACGACG1451.546141E-1017.8620685
GACCATG852.7226268E-517.4117647
TCTATAC752.0669778E-417.2666663
GTGTAAG1107.8054654E-716.8181821
GACGGTA1352.2182576E-816.4444458
ACGGTAT1352.2182576E-816.4444459
GTTCCGG803.3820962E-416.18751
GGTATCA42450.015.950531
TATAGTG1201.9348809E-615.4166665
CGACGGT1455.3425538E-815.3103457
ACCCACG1954.1836756E-1115.17948714
CCCACGG2201.8189894E-1215.13636415
AGTCGGT1601.0955773E-815.03125111
AGTACTC750.00410398314.85
CGGTGAT1651.6438207E-814.57575714
Produced by FastQC (version 0.11.5)