##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630784.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1019625
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.866655878386663	31.0	31.0	33.0	28.0	34.0
2	31.0412632095133	31.0	31.0	34.0	28.0	34.0
3	31.102369008213802	31.0	31.0	34.0	27.0	34.0
4	34.9323555228638	35.0	35.0	37.0	32.0	37.0
5	34.40146328306976	35.0	35.0	37.0	30.0	37.0
6	34.568457030771114	35.0	35.0	37.0	31.0	37.0
7	34.51986171386539	35.0	35.0	37.0	30.0	37.0
8	34.532009317150916	35.0	35.0	37.0	30.0	37.0
9	36.00305308324139	37.0	35.0	39.0	32.0	39.0
10	35.68752139266887	37.0	35.0	39.0	30.0	39.0
11	35.958872134363126	37.0	35.0	39.0	31.0	39.0
12	35.82881745739855	37.0	35.0	39.0	30.0	39.0
13	35.961514282211596	37.0	35.0	39.0	31.0	39.0
14	36.86616746352826	38.0	36.0	40.0	31.0	41.0
15	36.83170675493441	38.0	36.0	40.0	31.0	41.0
16	36.75593576069633	38.0	36.0	40.0	31.0	41.0
17	36.74878999632218	38.0	36.0	40.0	31.0	41.0
18	36.73315385558416	38.0	36.0	40.0	31.0	41.0
19	36.81247909770749	38.0	36.0	40.0	31.0	41.0
20	36.78057324996935	38.0	36.0	40.0	31.0	41.0
21	36.73015863675371	38.0	36.0	40.0	31.0	41.0
22	36.63717543214417	38.0	35.0	40.0	31.0	41.0
23	36.541344121613335	38.0	35.0	40.0	31.0	41.0
24	36.436739977933065	38.0	35.0	40.0	30.0	41.0
25	36.350465121981124	38.0	35.0	40.0	30.0	41.0
26	36.10402353806546	38.0	35.0	40.0	30.0	41.0
27	35.90542699521883	38.0	34.0	40.0	29.0	41.0
28	35.77362167463528	38.0	34.0	40.0	29.0	41.0
29	35.61177099423808	38.0	34.0	40.0	28.0	41.0
30	35.46301434350864	38.0	34.0	40.0	27.0	41.0
31	35.340970454824074	38.0	34.0	40.0	27.0	41.0
32	35.1659904376609	38.0	34.0	40.0	27.0	41.0
33	35.017545666298886	37.0	33.0	40.0	27.0	41.0
34	34.92208311879367	37.0	33.0	40.0	26.0	41.0
35	34.797804094642636	37.0	33.0	40.0	26.0	40.0
36	34.71155866127253	37.0	33.0	40.0	25.0	40.0
37	34.48078264067672	37.0	33.0	40.0	25.0	40.0
38	34.233127865636874	37.0	33.0	39.0	24.0	40.0
39	34.08971141350987	37.0	33.0	39.0	24.0	40.0
40	33.79595831800907	37.0	33.0	39.0	23.0	40.0
41	33.637118548485965	37.0	32.0	39.0	22.0	40.0
42	33.41561260267255	37.0	32.0	39.0	21.0	40.0
43	32.59031604756651	36.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	1.0
13	3.0
14	12.0
15	12.0
16	38.0
17	102.0
18	177.0
19	356.0
20	676.0
21	1164.0
22	1975.0
23	3136.0
24	4906.0
25	7434.0
26	10473.0
27	14804.0
28	20005.0
29	26410.0
30	34189.0
31	42773.0
32	54224.0
33	69099.0
34	90463.0
35	119286.0
36	156588.0
37	175746.0
38	137280.0
39	48286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81632953291651	21.905406399411547	13.195145273997793	22.083118793674146
2	18.426774549466714	23.6993012136815	34.511609660414365	23.362314576437416
3	21.867941645212703	26.2285644231948	29.694152261860978	22.20934166973152
4	15.339659188427119	16.894054186588207	34.388034816721834	33.378251808262846
5	13.021159740100527	39.80787054063994	33.60058845163663	13.5703812676229
6	32.64690449920314	35.17518695598872	16.5421601078828	15.63574843692534
7	26.209930121368153	32.94132646806424	22.935490989334316	17.9132524212333
8	27.0404315312002	33.48505578031139	21.270859384577665	18.20365330391075
9	26.564276081892853	14.026921662375875	22.281721221037145	37.12708103469413
10	17.19014343508643	28.60639941154836	33.05901679539046	21.144440357974744
11	32.29785460340812	23.347603285521636	22.553782027706266	21.800760083363983
12	20.39053573617752	28.30393527031997	30.442662743655756	20.862866249846757
13	28.841583915655267	21.472207919578278	27.76108863552777	21.92511952923869
14	21.98033590780924	23.099767071227166	28.410739242368514	26.50915777859507
15	24.824420742920193	28.26313595684688	25.83714600956234	21.075297290670587
16	22.62341547137428	27.657128846389607	27.675763148216255	22.04369253401986
17	21.11335049650607	28.117690327326223	28.746941277430427	22.022017898737282
18	20.650140983204608	25.749466715704305	31.127326222876057	22.47306607821503
19	21.420718401373055	26.918229741326467	31.377418168444283	20.2836336888562
20	22.411278656368765	25.550668137795757	31.43616525683462	20.601887949000858
21	22.79818560745372	26.47404683094275	30.0595807282089	20.668186833394632
22	22.415593968370725	26.413436312369743	30.32703199705774	20.843937722201787
23	21.6428588942013	26.620571288463896	30.538089984062765	21.198479833272035
24	21.656393281843815	27.25070491602305	30.34831433124923	20.744587470883904
25	21.590878999632217	26.726786808875815	30.56349147971068	21.11884271178129
26	21.331175677332354	27.370454824077477	30.276425156307468	21.0219443422827
27	21.489665318131664	27.06387152139267	30.155694495525314	21.29076866495035
28	21.05685913938948	27.127326222876057	30.86369988966532	20.952114748069143
29	20.62689714355768	27.623881328919946	30.914502880961138	20.834718646561235
30	20.725855093784478	28.119946058599975	30.536422704425647	20.617776143189896
31	21.000760083363982	28.024518818192963	30.252494789751132	20.722226308691923
32	20.361701605982592	27.89211719995096	30.37116586980508	21.37501532426137
33	20.375137918352333	27.762167463528257	30.827313963466963	21.035380654652446
34	21.006252298639204	27.465293612847862	30.287017285766826	21.241436802746108
35	20.788132891994607	27.553070981978667	30.60664459973029	21.052151526296434
36	20.655829349025375	28.00166727963712	29.92070614196396	21.421797229373542
37	21.132082873605494	27.411156062277797	29.84028441829104	21.61647664582567
38	20.65288709084222	27.383989211719996	30.470810346941278	21.492313350496506
39	20.889640799313472	27.19715581708962	30.48944464876793	21.423758734828983
40	20.444379060929265	27.07220791957828	30.873605492215273	21.609807527277187
41	20.059727841118058	27.401250459727837	30.812504597278412	21.72651710187569
42	20.41662375873483	27.07711168321687	30.680397204854724	21.825867353193576
43	19.999803849454455	26.880372686036537	30.374402353806545	22.745421110702463
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	608.0
1	489.5
2	371.0
3	823.0
4	1275.0
5	1275.0
6	1932.0
7	2589.0
8	2826.0
9	3063.0
10	4504.5
11	5946.0
12	5946.0
13	9878.0
14	13810.0
15	18890.5
16	23971.0
17	23029.5
18	22088.0
19	22088.0
20	23600.0
21	25112.0
22	22659.0
23	20206.0
24	21383.5
25	22561.0
26	22561.0
27	24806.5
28	27052.0
29	31635.5
30	36219.0
31	38362.0
32	40505.0
33	40505.0
34	42827.0
35	45149.0
36	47847.5
37	50546.0
38	53006.5
39	55467.0
40	55467.0
41	57903.0
42	60339.0
43	59707.0
44	59075.0
45	60154.0
46	61233.0
47	61233.0
48	62162.0
49	63091.0
50	61905.5
51	60720.0
52	60990.5
53	61261.0
54	61261.0
55	58298.0
56	55335.0
57	52418.0
58	49501.0
59	45333.0
60	41165.0
61	41165.0
62	37478.5
63	33792.0
64	29821.0
65	25850.0
66	21883.0
67	17916.0
68	17916.0
69	15246.0
70	12576.0
71	10755.5
72	8935.0
73	6999.0
74	5063.0
75	5063.0
76	3934.0
77	2805.0
78	2310.0
79	1815.0
80	1446.0
81	1077.0
82	1077.0
83	839.0
84	601.0
85	503.5
86	406.0
87	368.0
88	330.0
89	330.0
90	246.0
91	162.0
92	94.5
93	27.0
94	17.0
95	7.0
96	7.0
97	5.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1019625.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.4980654660084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.36637130293383	58.53324438059174
2	6.723792424411192	8.807907927878711
3	1.4858983251839775	2.9197039733619716
4	0.6247156803457266	1.6367067411570548
5	0.35811118884961585	1.1727795045691116
6	0.21214180431366508	0.8336926672208145
7	0.15621846482922247	0.716240506546862
8	0.11812073304819036	0.618934360486662
9	0.09454917639349733	0.5573509330660616
>10	0.6987159715906239	9.30750168122251
>50	0.10037472851684087	4.531948965772858
>100	0.05828620091778929	6.411164009139127
>500	0.0016524436291624738	0.7231856166936599
>1k	4.5066644431703836E-4	0.4156103462468054
>5k	6.008885924227178E-4	2.8140283860460777
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8613	0.8447223243839647	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8008	0.785386784356994	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6932	0.6798577908544808	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5047	0.49498590167953904	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1629	0.15976461934534755	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1362	0.13357852151526295	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1233	0.120926811327694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.001471129091577786	0.0
3	0.0	0.0	0.0	0.0025499570920681624	0.0
4	0.0	0.0	0.0	0.0038249356381022436	0.0
5	0.0	2.942258183155572E-4	0.0	0.00451146254750521	0.0
6	0.0	2.942258183155572E-4	0.0	0.004707613093048915	0.0
7	0.0	2.942258183155572E-4	0.0	0.005197989456908177	0.0
8	0.0	2.942258183155572E-4	0.0	0.005786441093539292	0.0
9	0.0	2.942258183155572E-4	0.0	0.008630624003923011	0.0
10	0.0	2.942258183155572E-4	0.0	0.013142086551428222	0.0
11	0.0	3.923010910874096E-4	0.0	0.01559396837072453	0.0
12	0.0	3.923010910874096E-4	0.0	0.017261248007846022	0.0
13	0.0	3.923010910874096E-4	0.0	0.018928527644967514	0.0
14	0.0	3.923010910874096E-4	0.0	0.02128233419149197	0.0
15	0.0	3.923010910874096E-4	0.0	0.02442074292019125	0.0
16	0.0	3.923010910874096E-4	0.0	0.030893710923133505	0.0
17	0.0	3.923010910874096E-4	0.0	0.03903395856319725	0.0
18	0.0	6.865269094029668E-4	0.0	0.04236851783744024	0.0
19	0.0	6.865269094029668E-4	0.0	0.048449184749295084	0.0
20	0.0	6.865269094029668E-4	0.0	0.054137550570062525	0.0
21	0.0	6.865269094029668E-4	0.0	0.06355277675616035	0.0
22	0.0	6.865269094029668E-4	0.0	0.07914674512688488	0.0
23	0.0	6.865269094029668E-4	0.0	0.10366556331984798	0.0
24	0.0	6.865269094029668E-4	0.0	0.13691308078950595	0.0
25	0.0	6.865269094029668E-4	0.0	0.14740713497609415	0.0
26	0.0	6.865269094029668E-4	0.0	0.16535490989334314	0.0
27	0.0	6.865269094029668E-4	0.0	0.18379306117445138	0.0
28	0.0	7.846021821748192E-4	0.0	0.221257815373299	0.0
29	0.0	7.846021821748192E-4	0.0	0.26450901066568594	0.0
30	0.0	8.826774549466716E-4	0.0	0.3441461321564301	0.0
31	0.0	8.826774549466716E-4	0.0	0.6063994115483634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	50	0.0070343493	18.5	30
GTCATAA	50	0.0070343493	18.5	1
ATTACGT	50	0.0070343493	18.5	17
TACGATG	50	0.0070343493	18.5	21
AATTACG	60	9.2358416E-4	18.5	16
TACGACG	145	1.546141E-10	17.862068	5
GACCATG	85	2.7226268E-5	17.411764	7
TCTATAC	75	2.0669778E-4	17.266666	3
GTGTAAG	110	7.8054654E-7	16.818182	1
GACGGTA	135	2.2182576E-8	16.444445	8
ACGGTAT	135	2.2182576E-8	16.444445	9
GTTCCGG	80	3.3820962E-4	16.1875	1
GGTATCA	4245	0.0	15.95053	1
TATAGTG	120	1.9348809E-6	15.416666	5
CGACGGT	145	5.3425538E-8	15.310345	7
ACCCACG	195	4.1836756E-11	15.179487	14
CCCACGG	220	1.8189894E-12	15.136364	15
AGTCGGT	160	1.0955773E-8	15.031251	11
AGTACTC	75	0.004103983	14.8	5
CGGTGAT	165	1.6438207E-8	14.575757	14
>>END_MODULE