FastQCFastQC Report
Fri 10 Feb 2017
ERR1630783.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630783.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835294
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86310.47027887629992793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75060.4089807954474869No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67990.3704583570806639No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49460.26949360701882097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA450.003826665720.5555552
TTATGCG555.144703E-420.1818184
GCGTTAT2750.016.8181821
GGTATCA39050.016.723431
TATACTG2055.456968E-1215.3414635
ATCTCGC855.366317E-415.23529411
TTGCGCG1353.9756014E-715.07407318
CGTTATT2850.014.9298242
TTAACGG1152.2111548E-514.47826135
GCGACTT908.277333E-414.38888822
AATACTG3900.014.230775
CCAATAC1702.4390829E-814.1470593
ATTTGCG1702.4390829E-814.14705916
TAGGGGT1051.6564799E-414.0952394
TAATACT3850.013.9350644
CGAATTA1601.7849561E-713.87515
ATGCGGA800.00630051113.8756
TACACAG2950.013.796615
TCTAGCG1907.1322575E-913.63157928
GAGTACG6300.013.50793651
Produced by FastQC (version 0.11.5)