##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630783.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1835294
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.120573597472664	33.0	31.0	34.0	30.0	34.0
2	32.29218370462716	34.0	31.0	34.0	30.0	34.0
3	32.39414175603473	34.0	31.0	34.0	30.0	34.0
4	35.954407304769695	37.0	35.0	37.0	35.0	37.0
5	35.9134187765012	37.0	35.0	37.0	35.0	37.0
6	35.99198548025548	37.0	35.0	37.0	35.0	37.0
7	35.94529159905716	37.0	35.0	37.0	35.0	37.0
8	35.94913512494456	37.0	35.0	37.0	35.0	37.0
9	37.66208138859496	39.0	37.0	39.0	35.0	39.0
10	37.578401607589846	39.0	37.0	39.0	35.0	39.0
11	37.67659949849997	39.0	37.0	39.0	35.0	39.0
12	37.63190475204517	39.0	37.0	39.0	35.0	39.0
13	37.67024956219549	39.0	37.0	39.0	35.0	39.0
14	38.9810242936554	40.0	38.0	41.0	36.0	41.0
15	38.985485159325975	40.0	38.0	41.0	36.0	41.0
16	38.954273266299566	40.0	38.0	41.0	36.0	41.0
17	38.94465082978531	40.0	38.0	41.0	36.0	41.0
18	38.935892559993114	40.0	38.0	41.0	36.0	41.0
19	38.99803301269442	40.0	39.0	41.0	35.0	41.0
20	38.964660702862865	40.0	38.0	41.0	35.0	41.0
21	38.93306957904292	40.0	38.0	41.0	35.0	41.0
22	38.86322027969361	40.0	38.0	41.0	35.0	41.0
23	38.80642120553982	40.0	38.0	41.0	35.0	41.0
24	38.75754674727864	40.0	38.0	41.0	35.0	41.0
25	38.70768552613369	40.0	38.0	41.0	35.0	41.0
26	38.57391295345596	40.0	38.0	41.0	34.0	41.0
27	38.450180189114114	40.0	38.0	41.0	34.0	41.0
28	38.35421954193715	40.0	38.0	41.0	34.0	41.0
29	38.275763992036154	40.0	38.0	41.0	34.0	41.0
30	38.189673698056005	40.0	38.0	41.0	34.0	41.0
31	38.137782829345056	40.0	38.0	41.0	34.0	41.0
32	38.03063487375864	40.0	37.0	41.0	33.0	41.0
33	37.94087759236395	40.0	37.0	41.0	33.0	41.0
34	37.877358069061415	40.0	37.0	41.0	33.0	41.0
35	37.808872039030256	40.0	37.0	41.0	33.0	41.0
36	37.76403072205325	40.0	37.0	41.0	33.0	41.0
37	37.630378566049906	40.0	37.0	41.0	33.0	41.0
38	37.472430030283974	40.0	37.0	41.0	32.0	41.0
39	37.39442726887354	40.0	36.0	41.0	32.0	41.0
40	37.215170430459644	40.0	36.0	41.0	31.0	41.0
41	37.14010234872451	40.0	36.0	41.0	31.0	41.0
42	37.00089631416002	40.0	36.0	41.0	31.0	41.0
43	36.226740783765436	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	4.0
11	8.0
12	4.0
13	5.0
14	5.0
15	8.0
16	11.0
17	18.0
18	49.0
19	96.0
20	212.0
21	411.0
22	766.0
23	1378.0
24	2185.0
25	3559.0
26	5458.0
27	8413.0
28	12215.0
29	17370.0
30	23920.0
31	31506.0
32	41447.0
33	54126.0
34	71695.0
35	97014.0
36	137987.0
37	217457.0
38	400214.0
39	707747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32826239283734	19.81758780881973	12.385209127256996	24.46894067108594
2	18.44418387462717	21.67004305577199	34.875992620255936	25.0097804493449
3	20.529517341635728	24.20048232054374	29.532216636680552	25.737783701139982
4	14.308497712083188	15.82433114258533	34.89604390359256	34.97112724173893
5	13.924853456721376	37.682627415553036	34.26039642694849	14.132122700777098
6	33.8495085800967	34.849457362144705	16.246497836313964	15.054536221444629
7	28.030440899387237	31.297928288328734	22.064366798997874	18.607264013286155
8	27.167472895350826	33.080204043602826	20.70011671154594	19.052206349500405
9	26.70237030143399	13.998683589659205	21.028565450549067	38.27038065835773
10	17.13278635466579	27.177988921665957	33.19495405095859	22.49427067270966
11	34.170492575031574	22.457382849832232	22.051616798180564	21.320507776955626
12	21.24575136190714	26.397732461393108	29.633998694487097	22.72251748221266
13	29.28511726186649	20.656853888259867	26.2363414254065	23.821687424467143
14	22.536825162616996	21.936267431812016	26.472270927709673	29.05463647786131
15	25.28254328734252	27.938902431980928	23.695059211221743	23.083495069454813
16	24.2609086064685	27.065636350361306	25.36334777970178	23.310107263468414
17	22.557148881868518	27.129713277545726	26.867902363327072	23.445235477258684
18	22.476071953594357	25.657633054976476	28.500392852589286	23.365902138839882
19	23.040068784619795	26.14878052235773	28.449665285234953	22.361485407787526
20	23.7628412668488	25.369559318561496	28.386133229880333	22.48146618470937
21	24.114447058618403	25.809161910843713	27.456636375425408	22.61975465511248
22	23.926956661984402	25.81308498801827	27.558963305061752	22.700995044935578
23	23.266680978633396	25.886043326028418	27.894713326584185	22.952562368754
24	23.103437378425472	26.30352412202078	27.703081904043714	22.88995659551004
25	23.336697008762627	25.993927948328714	27.640803053897635	23.028571989011027
26	22.94945659932414	26.723892738711076	27.62494728365047	22.70170337831432
27	23.34399829128194	26.235469630478825	27.30734149406035	23.113190584178884
28	22.746655304272775	26.274046556028622	28.09849539092919	22.88080274876941
29	22.354238612451194	26.561411959064866	28.28505950545253	22.799289923031406
30	22.382789896332685	27.05964276023351	28.161700523185928	22.39586682024787
31	22.68797260820337	26.958569035805706	27.473527402149195	22.87993095384173
32	22.248478990287115	26.659107478147913	27.90811717359725	23.184296357967714
33	22.143318727135817	26.55705298442647	28.293450531631443	23.006177756806267
34	22.988578396703744	26.154665138119558	27.878421658873183	22.978334806303515
35	22.59812324346944	26.286578608114013	28.387113999173973	22.728184149242573
36	22.173722575238628	26.679213248667516	27.876405633102923	23.270658542990933
37	22.767196972256215	25.740562547471956	28.1511844968708	23.341055983401024
38	22.292940531598752	25.68558497984519	28.650777477613943	23.370697010942116
39	22.292395659768953	25.836078579235807	28.599831961527688	23.271693799467553
40	21.79214883283005	25.666623440168166	29.29743136521996	23.24379636178182
41	21.285799441397398	25.691360621241067	29.565399331115344	23.457440606246195
42	21.674728953508264	25.763283702774597	29.339114060199616	23.22287328351752
43	21.198075076799686	25.325097777249855	29.458549965291663	24.018277180658792
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	690.0
1	591.5
2	493.0
3	1111.0
4	1729.0
5	1729.0
6	2382.0
7	3035.0
8	3002.5
9	2970.0
10	4276.0
11	5582.0
12	5582.0
13	9553.5
14	13525.0
15	17632.0
16	21739.0
17	21736.5
18	21734.0
19	21734.0
20	25388.0
21	29042.0
22	28225.5
23	27409.0
24	30678.5
25	33948.0
26	33948.0
27	37924.5
28	41901.0
29	47035.0
30	52169.0
31	58487.5
32	64806.0
33	64806.0
34	70989.5
35	77173.0
36	83313.0
37	89453.0
38	94894.0
39	100335.0
40	100335.0
41	103721.5
42	107108.0
43	107393.5
44	107679.0
45	109193.5
46	110708.0
47	110708.0
48	112368.5
49	114029.0
50	115471.5
51	116914.0
52	119250.0
53	121586.0
54	121586.0
55	118016.0
56	114446.0
57	109330.0
58	104214.0
59	96162.0
60	88110.0
61	88110.0
62	82088.5
63	76067.0
64	67506.0
65	58945.0
66	50785.0
67	42625.0
68	42625.0
69	36632.0
70	30639.0
71	26402.0
72	22165.0
73	17661.0
74	13157.0
75	13157.0
76	10288.0
77	7419.0
78	6167.5
79	4916.0
80	3938.0
81	2960.0
82	2960.0
83	2309.5
84	1659.0
85	1367.5
86	1076.0
87	902.0
88	728.0
89	728.0
90	536.0
91	344.0
92	196.0
93	48.0
94	31.5
95	15.0
96	15.0
97	9.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1835294.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.71481474163922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.09603312833471	53.134007345052105
2	8.822823479698709	10.88997833095581
3	2.143806566394054	3.969138752607562
4	0.8579132299733296	2.1178382420882147
5	0.4437507272303388	1.369299696124402
6	0.2770596040288234	1.0259209281018455
7	0.19082456595215957	0.824369191512387
8	0.14689708521918737	0.7252581120311151
9	0.11619000111814277	0.6453579954453331
>10	0.7306320286968373	8.725975944548958
>50	0.09177368042870859	3.986990970467897
>100	0.07742945979060464	9.09257071264088
>500	0.004070116076073362	1.6263231144824535
>1k	5.308847055747864E-4	0.6145331340155635
>5k	2.654423527873932E-4	1.2524375299254458
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8631	0.47027887629992793	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7506	0.4089807954474869	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6799	0.3704583570806639	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4946	0.26949360701882097	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0897436595989526E-4	0.0
2	0.0	0.0	0.0	0.0011987180255588479	0.0
3	0.0	0.0	0.0	0.002179487319197905	0.0
4	0.0	0.0	0.0	0.0037051284426364386	0.0
5	0.0	0.0	0.0	0.0041955130894559675	0.0
6	0.0	0.0	0.0	0.00435897463839581	0.0
7	0.0	0.0	0.0	0.004903846468195286	0.0
8	0.0	0.0	0.0	0.005339743932034868	0.0
9	0.0	0.0	0.0	0.008118590264012196	0.0
10	0.0	0.0	0.0	0.011060898144929368	0.0
11	5.448718297994763E-5	0.0	0.0	0.012804488000287693	0.0
12	5.448718297994763E-5	0.0	0.0	0.013948718842866593	0.0
13	5.448718297994763E-5	0.0	0.0	0.014820513770545754	0.0
14	5.448718297994763E-5	0.0	0.0	0.0160192317961046	0.0
15	5.448718297994763E-5	0.0	0.0	0.018580129396162142	0.0
16	5.448718297994763E-5	0.0	0.0	0.02228525783879858	0.0
17	5.448718297994763E-5	0.0	0.0	0.02566346318355533	0.0
18	5.448718297994763E-5	0.0	0.0	0.027951924868713132	0.0
19	5.448718297994763E-5	5.448718297994763E-5	0.0	0.0320384635922092	0.0
20	5.448718297994763E-5	5.448718297994763E-5	0.0	0.03465384837524669	0.0
21	5.448718297994763E-5	5.448718297994763E-5	0.0	0.04081090005198077	0.0
22	5.448718297994763E-5	5.448718297994763E-5	0.0	0.048875003133013024	0.0
23	5.448718297994763E-5	5.448718297994763E-5	0.0	0.05900961916728328	0.0
24	5.448718297994763E-5	5.448718297994763E-5	0.0	0.0781346203932449	0.0
25	5.448718297994763E-5	5.448718297994763E-5	0.0	0.08418269770401908	0.0
26	5.448718297994763E-5	5.448718297994763E-5	0.0	0.0955160317638482	0.0
27	5.448718297994763E-5	5.448718297994763E-5	0.0	0.11578526383238871	0.0
28	5.448718297994763E-5	5.448718297994763E-5	0.0	0.1540897534672919	0.0
29	5.448718297994763E-5	1.0897436595989526E-4	0.0	0.2031827053322247	0.0
30	5.448718297994763E-5	1.0897436595989526E-4	0.0	0.2852948900830058	0.0
31	5.448718297994763E-5	1.0897436595989526E-4	0.0	0.5537532406252077	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATA	45	0.0038266657	20.555555	2
TTATGCG	55	5.144703E-4	20.181818	4
GCGTTAT	275	0.0	16.818182	1
GGTATCA	3905	0.0	16.72343	1
TATACTG	205	5.456968E-12	15.341463	5
ATCTCGC	85	5.366317E-4	15.235294	11
TTGCGCG	135	3.9756014E-7	15.074073	18
CGTTATT	285	0.0	14.929824	2
TTAACGG	115	2.2111548E-5	14.478261	35
GCGACTT	90	8.277333E-4	14.388888	22
AATACTG	390	0.0	14.23077	5
CCAATAC	170	2.4390829E-8	14.147059	3
ATTTGCG	170	2.4390829E-8	14.147059	16
TAGGGGT	105	1.6564799E-4	14.095239	4
TAATACT	385	0.0	13.935064	4
CGAATTA	160	1.7849561E-7	13.875	15
ATGCGGA	80	0.006300511	13.875	6
TACACAG	295	0.0	13.79661	5
TCTAGCG	190	7.1322575E-9	13.631579	28
GAGTACG	630	0.0	13.5079365	1
>>END_MODULE