FastQCFastQC Report
Fri 10 Feb 2017
ERR1630782.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630782.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595053
Sequences flagged as poor quality0
Sequence length43
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236963.982166294430916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT212793.575983987980903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT193533.2523153399781193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136652.2964340991474708No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42500.7142220945025065No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37050.6226336141486557No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31030.5214661551155947No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT25830.434078981199994No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22620.38013420653286345No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22390.3762690046096734No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA19600.32938242475880297No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.3011496454937627No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA16180.27190855268354247No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA15330.25762411079349234No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.24989370694711224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14330.24081888504049218No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA13610.22871912249833207No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.22216508445466202No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA12220.20535985870166187No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA11400.19157957358420175No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA11130.1870421626308917No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT10010.16822030978753152No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9950.1672119962423515No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA9920.1667078394697615No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA9690.1628426375465715No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA8260.13881116471978128No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA8230.13830700794719128No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8160.13713064214448126No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT8000.13444180602400121No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC7780.13074465635834118No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA7680.12906413378304118No Hit
GTACATGGGAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAA7130.11982125961889109No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6690.11242696028757103No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA6590.11074643771227101No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG6510.109402019652031No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6490.10906591513697099No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA6470.10872981062191099No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA6120.10284798160836095No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA6060.10183966806318094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCTT200.001841222437.026
ACACCTT358.8643504E-426.428576
GTTATAC509.08147E-625.9000023
GCGTTAT701.9196887E-723.7857131
ATGGTCT551.9006602E-523.54545427
GCAGCGT952.8012437E-1023.3684221
GCCTATG400.001930098223.1250021
CGTTATA603.7224003E-521.5833322
TTGAACT450.003823671720.5555574
TACACTG851.2432447E-619.5882365
AGCGTCA1052.2539098E-819.3809533
CCGCTTA1551.8189894E-1219.09677525
TATTGAC500.00703099318.530
ATTCCCC801.6145355E-518.537
ATTGACT500.00703099318.531
ATGCCCC1156.389564E-817.69565237
CATGGTC752.0651559E-417.26666626
TAGGGCT752.0651559E-417.2666664
ATGGAAG650.001578723317.0769235
CTCCGCT1901.8189894E-1216.55263123
Produced by FastQC (version 0.11.5)