##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630782.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595053
Sequences flagged as poor quality	0
Sequence length	43
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.960521163661053	33.0	31.0	34.0	30.0	34.0
2	31.968880082950594	33.0	31.0	34.0	30.0	34.0
3	31.954326757448495	33.0	31.0	34.0	30.0	34.0
4	35.6429192021551	37.0	35.0	37.0	33.0	37.0
5	35.66707335312989	37.0	35.0	37.0	35.0	37.0
6	35.84277198837751	37.0	35.0	37.0	35.0	37.0
7	35.72775198175625	37.0	35.0	37.0	35.0	37.0
8	35.85289041480339	37.0	35.0	37.0	35.0	37.0
9	37.347631219403986	39.0	37.0	39.0	34.0	39.0
10	37.34498775739304	39.0	37.0	39.0	34.0	39.0
11	37.499616000591544	39.0	37.0	39.0	35.0	39.0
12	37.524785187201815	39.0	37.0	39.0	35.0	39.0
13	37.55046189162982	39.0	37.0	39.0	35.0	39.0
14	38.64930182689609	40.0	38.0	41.0	34.0	41.0
15	38.53356759818033	40.0	38.0	41.0	34.0	41.0
16	38.40114578029184	40.0	38.0	41.0	34.0	41.0
17	38.638198614241084	40.0	38.0	41.0	35.0	41.0
18	38.74710656025598	40.0	38.0	41.0	35.0	41.0
19	38.92839965515677	40.0	38.0	41.0	35.0	41.0
20	38.95211350921683	40.0	39.0	41.0	35.0	41.0
21	38.90551261820376	40.0	38.0	41.0	35.0	41.0
22	38.74860726691572	40.0	38.0	41.0	35.0	41.0
23	38.59728797266798	40.0	38.0	41.0	35.0	41.0
24	38.462547033625576	40.0	38.0	41.0	35.0	41.0
25	38.33510124308255	40.0	37.0	41.0	34.0	41.0
26	37.93575026090113	40.0	36.0	41.0	34.0	41.0
27	37.604801589102145	40.0	35.0	41.0	33.0	41.0
28	37.36243662329238	39.0	35.0	41.0	33.0	41.0
29	37.07557982230154	39.0	35.0	41.0	33.0	41.0
30	36.70173413124545	38.0	35.0	41.0	33.0	41.0
31	36.250599526428736	38.0	35.0	40.0	32.0	41.0
32	35.80620717818413	37.0	35.0	40.0	31.0	41.0
33	35.38725962225214	36.0	35.0	40.0	30.0	41.0
34	35.03440029711639	35.0	35.0	40.0	28.0	41.0
35	34.62392593600906	35.0	35.0	40.0	25.0	41.0
36	34.1579489558073	35.0	34.0	40.0	21.0	41.0
37	33.5494972716716	35.0	33.0	40.0	18.0	41.0
38	32.92506213732222	35.0	33.0	40.0	15.0	41.0
39	32.316810435372986	35.0	33.0	40.0	10.0	41.0
40	31.578819029565434	35.0	31.0	40.0	8.0	41.0
41	30.894535444741898	35.0	30.0	39.0	7.0	41.0
42	30.14797505432289	35.0	25.0	39.0	7.0	41.0
43	29.267818160735263	35.0	22.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	7.0
11	9.0
12	8.0
13	10.0
14	5.0
15	6.0
16	8.0
17	14.0
18	44.0
19	105.0
20	217.0
21	485.0
22	795.0
23	1312.0
24	2267.0
25	3599.0
26	5446.0
27	7960.0
28	11295.0
29	14687.0
30	17878.0
31	21182.0
32	25109.0
33	30320.0
34	37161.0
35	43707.0
36	58551.0
37	109932.0
38	105729.0
39	97201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.311217656242384	20.099050000588186	13.248231670120141	13.341500673049293
2	16.023614703228116	26.67644730805491	33.24997941359845	24.04995857511852
3	26.33177212786088	32.202341640156426	27.809623680579715	13.656262551402984
4	13.598452574812663	17.74547813388051	29.005651597420734	39.65041769388609
5	9.671407420851589	48.0582401903696	27.36041999620202	14.909932392576797
6	29.20445741807873	28.529223447323183	20.039895605937623	22.226423528660472
7	22.37464561980193	39.39077695600224	22.778139090131468	15.456438334064359
8	35.33248298891023	27.513179498296793	20.110141449585164	17.044196063207814
9	30.449388541860976	10.871132487358269	25.37051321478927	33.30896575599148
10	21.11240511349409	33.84807739814773	25.13473589747468	19.904781590883502
11	24.220699668769	27.691146839021062	19.58833918995451	28.499814302255427
12	20.7573106933332	34.97469973262886	29.87666644819873	14.391323125839211
13	29.062285208208344	21.515058322535978	33.518022764358804	15.904633704896876
14	23.698561304623286	24.650241239015685	35.44020448598697	16.210992970374065
15	27.755174749139993	23.03357852157707	34.27257740066851	14.938669328614425
16	17.837570771006952	25.795013217310053	37.97796162694752	18.389454384735476
17	15.60852562712901	30.89909638301126	35.780678359742744	17.711699630116982
18	13.778772647142357	23.816533989409347	41.88652103258029	20.518172330868005
19	15.143693082801027	28.09581667515331	44.658374968280135	12.10211527376553
20	17.040330861284623	23.09508564783305	45.903474144319915	13.961109346562406
21	19.840921733022103	24.22221213908677	42.57368671362047	13.363179414270663
22	18.493142627631485	25.23472699070503	41.87475737455319	14.397373007110293
23	16.081928836591025	27.150354674289517	41.90030131769775	14.867415171421705
24	13.945480486612116	29.247982952778994	42.763921869144426	14.042614691464458
25	13.796250081925477	28.855076774673854	42.37034348200916	14.978329661391507
26	14.778851631703393	28.527879029262937	42.26850381394598	14.424765525087683
27	13.411746516696832	30.434599943198336	42.06062317138137	14.093030368723458
28	13.275120031324942	30.19193248332501	43.32773719315758	13.20521029219246
29	12.731639030472916	31.349980589964254	43.246736005028126	12.671644374534704
30	12.025315392074319	32.734899244269	42.92155488670757	12.318230476949111
31	11.835080236550358	33.12881373591932	41.96970690005764	13.066399127472678
32	10.956503034183509	34.374585120989224	42.08566295775334	12.583248887073925
33	11.226731064291751	34.27240934841098	41.79627697028668	12.704582617010585
34	11.831887243657288	34.910671822509926	39.777465200578774	13.479975733254012
35	11.418982846906074	35.39802336934693	39.456653440954	13.726340342792994
36	11.417974533360894	35.456169450452315	38.41691412361588	14.708941892570914
37	11.613755413383346	35.86285591367492	38.208865428793736	14.314523244148
38	11.876421091902738	34.91369676314547	38.2713808685949	14.938501276356897
39	12.496870026703503	35.28996576775514	38.06770153246853	14.14546267307282
40	11.9070066027732	35.38138619585146	37.75999784893111	14.951609352444237
41	11.416125958528065	36.42045330415946	36.78428644171191	15.379134295600561
42	11.783320141231117	35.91226327738873	36.643458649901774	15.660957931478373
43	11.284204936367011	36.66597765241079	35.5776712326465	16.472146178575688
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	150.5
2	257.0
3	1434.0
4	2611.0
5	2611.0
6	4349.0
7	6087.0
8	6596.0
9	7105.0
10	10296.5
11	13488.0
12	13488.0
13	23751.5
14	34015.0
15	45600.0
16	57185.0
17	52279.5
18	47374.0
19	47374.0
20	48329.5
21	49285.0
22	35789.5
23	22294.0
24	19430.0
25	16566.0
26	16566.0
27	17467.5
28	18369.0
29	18789.0
30	19209.0
31	18827.5
32	18446.0
33	18446.0
34	17373.5
35	16301.0
36	15709.0
37	15117.0
38	16703.0
39	18289.0
40	18289.0
41	20016.0
42	21743.0
43	23004.5
44	24266.0
45	27903.5
46	31541.0
47	31541.0
48	33554.0
49	35567.0
50	31661.0
51	27755.0
52	21663.0
53	15571.0
54	15571.0
55	14528.0
56	13485.0
57	12787.0
58	12089.0
59	11478.0
60	10867.0
61	10867.0
62	10137.0
63	9407.0
64	8715.5
65	8024.0
66	7435.0
67	6846.0
68	6846.0
69	6149.5
70	5453.0
71	4738.0
72	4023.0
73	3345.5
74	2668.0
75	2668.0
76	2179.0
77	1690.0
78	1347.5
79	1005.0
80	751.0
81	497.0
82	497.0
83	402.0
84	307.0
85	215.0
86	123.0
87	94.5
88	66.0
89	66.0
90	40.0
91	14.0
92	8.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	595053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.80234531958687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.05775674646148	26.817906598430767
2	11.427750577917887	7.954250436776135
3	3.945272810413605	4.119142401839737
4	2.008184661951204	2.795581442828945
5	1.146558511563725	1.9951462624276148
6	0.7472349902835104	1.560331810003492
7	0.5461902686090723	1.3306091636831587
8	0.3996908212359175	1.112814238537733
9	0.32804753614486	1.0275141270737767
>10	2.0196844209102567	13.601813084226372
>50	0.19842972584002602	4.790449319012838
>100	0.15245210643798976	10.886940760999734
>500	0.012099373526824586	2.9546802838891226
>1k	0.008711548939313701	5.916097952988345
>5k	0.0	0.0
>10k+	0.0019358997642919336	13.136722117282199
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23696	3.982166294430916	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21279	3.575983987980903	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19353	3.2523153399781193	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13665	2.2964340991474708	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4250	0.7142220945025065	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3705	0.6226336141486557	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3103	0.5214661551155947	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	2583	0.434078981199994	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2262	0.38013420653286345	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	2239	0.3762690046096734	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1960	0.32938242475880297	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1792	0.3011496454937627	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1618	0.27190855268354247	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1533	0.25762411079349234	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1487	0.24989370694711224	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	1433	0.24081888504049218	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	1361	0.22871912249833207	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1322	0.22216508445466202	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	1222	0.20535985870166187	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1140	0.19157957358420175	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	1113	0.1870421626308917	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	1001	0.16822030978753152	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	995	0.1672119962423515	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	992	0.1667078394697615	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	969	0.1628426375465715	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	826	0.13881116471978128	No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA	823	0.13830700794719128	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	816	0.13713064214448126	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	800	0.13444180602400121	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	778	0.13074465635834118	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	768	0.12906413378304118	No Hit
GTACATGGGAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAA	713	0.11982125961889109	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	669	0.11242696028757103	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	659	0.11074643771227101	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	651	0.109402019652031	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	649	0.10906591513697099	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	647	0.10872981062191099	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	612	0.10284798160836095	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	606	0.10183966806318094	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6805225753000152E-4	0.0
2	0.0	0.0	0.0	0.0045374109533100416	0.0
3	0.0	0.0	0.0	0.006385985786140058	0.0
4	0.0	0.0	0.0	0.010419239966860094	0.0
5	0.0	0.0	0.0	0.01193171028463011	0.0
6	0.0	0.0	0.0	0.012267814799690111	0.0
7	0.0	0.0	0.0	0.013444180602400123	0.0
8	0.0	0.0	0.0	0.014956650920170136	0.0
9	0.0	0.0	0.0	0.025880047659620237	0.0
10	0.0	3.3610451506000305E-4	0.0	0.03747565342919034	0.0
11	0.0	3.3610451506000305E-4	0.0	0.043357482442740396	0.0
12	0.0	3.3610451506000305E-4	0.0	0.050079572743940454	0.0
13	0.0	3.3610451506000305E-4	0.0	0.0551211404698405	0.0
14	0.0	3.3610451506000305E-4	0.0	0.06486817140658059	0.0
15	0.0	3.3610451506000305E-4	0.0	0.07915261329663072	0.0
16	0.0	3.3610451506000305E-4	0.0	0.1206615209065411	0.0
17	0.0	6.722090301200061E-4	0.0	0.17225356396825156	0.0
18	0.0	6.722090301200061E-4	0.0	0.19477256647727179	0.0
19	0.0	6.722090301200061E-4	0.0	0.24014667601037218	0.0
20	0.0	6.722090301200061E-4	0.0	0.2636739920645724	0.0
21	0.0	8.402612876500077E-4	0.0	0.332071260879283	0.0
22	0.0	0.0010083135451800093	0.0	0.4456745869695641	0.0
23	0.0	0.0010083135451800093	0.0	0.6212891960884157	0.0
24	0.0	0.0010083135451800093	0.0	0.8671496488548079	0.0
25	0.0	0.0010083135451800093	0.0	0.9316817157463285	0.0
26	0.0	0.0010083135451800093	0.0	1.0434364670037795	0.0
27	0.0	0.0010083135451800093	0.0	1.1126739971061401	0.0
28	0.0	0.0010083135451800093	0.0	1.195019603295841	0.0
29	0.0	0.0010083135451800093	0.0	1.311311765506602	0.0
30	0.0	0.0010083135451800093	0.0	1.4881027404281635	0.0
31	0.0	0.0010083135451800093	0.0	1.9621781589202978	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTCTT	20	0.0018412224	37.0	26
ACACCTT	35	8.8643504E-4	26.42857	6
GTTATAC	50	9.08147E-6	25.900002	3
GCGTTAT	70	1.9196887E-7	23.785713	1
ATGGTCT	55	1.9006602E-5	23.545454	27
GCAGCGT	95	2.8012437E-10	23.368422	1
GCCTATG	40	0.0019300982	23.125002	1
CGTTATA	60	3.7224003E-5	21.583332	2
TTGAACT	45	0.0038236717	20.555557	4
TACACTG	85	1.2432447E-6	19.588236	5
AGCGTCA	105	2.2539098E-8	19.380953	3
CCGCTTA	155	1.8189894E-12	19.096775	25
TATTGAC	50	0.007030993	18.5	30
ATTCCCC	80	1.6145355E-5	18.5	37
ATTGACT	50	0.007030993	18.5	31
ATGCCCC	115	6.389564E-8	17.695652	37
CATGGTC	75	2.0651559E-4	17.266666	26
TAGGGCT	75	2.0651559E-4	17.266666	4
ATGGAAG	65	0.0015787233	17.076923	5
CTCCGCT	190	1.8189894E-12	16.552631	23
>>END_MODULE