FastQCFastQC Report
Fri 10 Feb 2017
ERR1630781.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630781.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010710
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86990.4326332489518627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79330.39453725301013076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61370.30521557061933346No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45250.2250448846427381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.001931687723.1250025
ATACCGT1900.017.5263166
CAAGACG2900.017.2241384
GACGGAC2650.016.7547157
GGTATCA42350.016.5997641
AGTCGGT2600.016.36538511
CTTATAC10150.015.85714337
TAGCGCA957.063884E-515.5789484
TCTATAC1557.21775E-915.51612953
CAGTCGG2750.015.47272710
CGGACCA3050.015.1639359
CGCAAGA3200.015.0312512
GCGCAAG3100.014.9193551
CTACTAG1651.646913E-814.5757571
TCTTATA18000.014.49166737
AAGACGG3350.014.3582095
ACGGACC3100.014.3225818
TACGTTA1551.2114288E-714.32258119
CGAATTA1304.4476637E-614.23076915
TTACACT2600.014.2307694
Produced by FastQC (version 0.11.5)