##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630781.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2010710
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09840951703627	33.0	31.0	34.0	30.0	34.0
2	32.27520428107484	34.0	31.0	34.0	30.0	34.0
3	32.375863749620784	34.0	31.0	34.0	30.0	34.0
4	35.942924638560505	37.0	35.0	37.0	35.0	37.0
5	35.90234892152523	37.0	35.0	37.0	35.0	37.0
6	35.97611788870598	37.0	35.0	37.0	35.0	37.0
7	35.93003168035172	37.0	35.0	37.0	35.0	37.0
8	35.93047978077396	37.0	35.0	37.0	35.0	37.0
9	37.64775477318957	39.0	37.0	39.0	35.0	39.0
10	37.56760000198935	39.0	37.0	39.0	35.0	39.0
11	37.660245883294955	39.0	37.0	39.0	35.0	39.0
12	37.61031824579378	39.0	37.0	39.0	35.0	39.0
13	37.64881111647129	39.0	37.0	39.0	35.0	39.0
14	38.96228148266035	40.0	38.0	41.0	36.0	41.0
15	38.98057750744762	40.0	38.0	41.0	36.0	41.0
16	38.93814125358704	40.0	38.0	41.0	36.0	41.0
17	38.924965310760875	40.0	38.0	41.0	36.0	41.0
18	38.91143476682366	40.0	38.0	41.0	35.0	41.0
19	38.979536084268744	40.0	38.0	41.0	35.0	41.0
20	38.94883349662557	40.0	38.0	41.0	35.0	41.0
21	38.91325452203451	40.0	38.0	41.0	35.0	41.0
22	38.847231077579565	40.0	38.0	41.0	35.0	41.0
23	38.79537974148435	40.0	38.0	41.0	35.0	41.0
24	38.74957800975775	40.0	38.0	41.0	35.0	41.0
25	38.70203112333454	40.0	38.0	41.0	34.0	41.0
26	38.571290240760725	40.0	38.0	41.0	34.0	41.0
27	38.44585693610715	40.0	38.0	41.0	34.0	41.0
28	38.357806943815866	40.0	38.0	41.0	34.0	41.0
29	38.2897404399441	40.0	38.0	41.0	34.0	41.0
30	38.20701941105381	40.0	38.0	41.0	34.0	41.0
31	38.15627464925325	40.0	38.0	41.0	34.0	41.0
32	38.05837539973442	40.0	37.0	41.0	33.0	41.0
33	37.98931621168642	40.0	37.0	41.0	33.0	41.0
34	37.92741767833253	40.0	37.0	41.0	33.0	41.0
35	37.86616966146287	40.0	37.0	41.0	33.0	41.0
36	37.82772751913503	40.0	37.0	41.0	33.0	41.0
37	37.69970458196359	40.0	37.0	41.0	33.0	41.0
38	37.54688990456108	40.0	37.0	41.0	33.0	41.0
39	37.48842548154632	40.0	37.0	41.0	32.0	41.0
40	37.31327889153582	40.0	36.0	41.0	32.0	41.0
41	37.24545011463612	40.0	36.0	41.0	32.0	41.0
42	37.12254079404787	40.0	36.0	41.0	31.0	41.0
43	36.34439426869116	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	10.0
11	9.0
12	6.0
13	6.0
14	2.0
15	5.0
16	9.0
17	15.0
18	55.0
19	107.0
20	250.0
21	490.0
22	846.0
23	1458.0
24	2478.0
25	3994.0
26	6084.0
27	9084.0
28	13498.0
29	19128.0
30	26253.0
31	34386.0
32	44904.0
33	58752.0
34	77678.0
35	104652.0
36	149066.0
37	235271.0
38	437161.0
39	785049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.578885070447754	19.19232509909435	12.36657698027065	24.862212850187248
2	18.37495213133669	21.198034525117993	35.26784071298198	25.159172630563333
3	20.28174127546986	23.456291558703143	29.896056616816946	26.365910549010053
4	14.514226318066752	15.665759855971274	34.28649581491115	35.53351801105082
5	14.155497311894806	37.40892520552442	34.223980583972825	14.211596898607953
6	34.57519980504399	34.48005928254199	15.957049997264647	14.987690915149376
7	28.55359549611829	31.041323711524786	21.563427843895937	18.84165294846099
8	26.939787438268077	33.84764585643877	20.31143228013985	18.901134425153305
9	26.543360305563706	14.02683629165817	20.309791068826435	39.120012333951685
10	16.84892401191619	27.3265662377966	33.29764113173954	22.526868618547677
11	34.59235792332061	22.107265592750817	21.804586439615857	21.495790044312706
12	20.952648566924122	26.17130267417977	29.603423666267137	23.27262509262897
13	29.54652834073536	20.267666645115405	26.04955463492995	24.13625037921928
14	22.728290007012447	21.520010344604643	25.878769190982293	29.87293045740062
15	25.510640520015315	27.791277707874336	23.174997886318764	23.523083885791586
16	24.632095130575767	26.87488499087387	24.757324527157074	23.73569535139329
17	23.049320886651977	27.104903243133023	26.238990207439162	23.606785662775838
18	22.594208016073924	25.56390528718711	27.93267054920899	23.90921614752998
19	23.56863993315794	25.940936286187465	27.903526614976798	22.586897165677797
20	24.08134440073407	25.092728439208038	27.946595978534944	22.879331181522943
21	24.02539401504941	25.724893196930438	26.89502712972035	23.354685658299804
22	24.078658782221204	25.54684663626281	26.885478263896832	23.48901631761915
23	23.421279050683587	25.893937962212355	27.24306339551701	23.441719591587052
24	23.62414271575712	26.160062863366672	27.096896121270596	23.11889829960561
25	23.884349309447906	25.599862735053787	27.103709634905083	23.412078320593224
26	23.530394736187716	26.31209871140045	27.023887084661634	23.133619467750197
27	23.933386714145747	25.732800851440533	26.583445648552008	23.750366785861708
28	23.099850301634746	26.028119420503202	27.381870085691123	23.490160192170926
29	22.84108598455272	26.43364781594561	27.457515007136784	23.267751192364887
30	22.778421552585904	27.00593322756638	27.38977774020122	22.82586747964649
31	23.361648372962783	26.472489817029803	26.959382506676743	23.206479303330667
32	22.57506055075073	26.454138090525237	27.258132699394743	23.712668659329292
33	22.776631140243992	26.15454242531245	27.54554361394731	23.52328282049624
34	23.32643693023857	25.937703597236794	27.276235757518492	23.459623715006142
35	23.252582421134825	26.109483714707736	27.63382088913866	23.004112975018774
36	22.76688333971582	26.375210746452744	27.309209184815312	23.548696729016118
37	23.445748019356348	25.449119962600275	27.334225223925877	23.7709067941175
38	22.785384267248883	25.639351273928114	28.049644155547043	23.525620303275957
39	22.902059471529956	25.62283969344162	27.96897613280881	23.506124702219612
40	22.34966753037484	25.342739629285177	28.72552481461772	23.582068025722258
41	21.843726842757036	25.600310338139266	28.974789999552396	23.581172819551302
42	22.459429753669102	25.25013552426755	28.577119524943928	23.713315197119424
43	21.801652152722173	25.199854777665603	28.724828543151425	24.273664526460802
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	339.0
1	320.5
2	302.0
3	873.0
4	1444.0
5	1444.0
6	1989.0
7	2534.0
8	2541.0
9	2548.0
10	3775.0
11	5002.0
12	5002.0
13	8577.0
14	12152.0
15	16772.5
16	21393.0
17	21027.0
18	20661.0
19	20661.0
20	24213.0
21	27765.0
22	26737.0
23	25709.0
24	28434.5
25	31160.0
26	31160.0
27	35394.5
28	39629.0
29	47586.0
30	55543.0
31	61120.5
32	66698.0
33	66698.0
34	72683.5
35	78669.0
36	84679.5
37	90690.0
38	99581.0
39	108472.0
40	108472.0
41	113436.5
42	118401.0
43	117151.5
44	115902.0
45	120970.5
46	126039.0
47	126039.0
48	129080.5
49	132122.0
50	136430.0
51	140738.0
52	146056.0
53	151374.0
54	151374.0
55	140396.0
56	129418.0
57	123078.5
58	116739.0
59	106300.0
60	95861.0
61	95861.0
62	89751.0
63	83641.0
64	74427.5
65	65214.0
66	56671.5
67	48129.0
68	48129.0
69	41259.0
70	34389.0
71	29731.5
72	25074.0
73	20262.5
74	15451.0
75	15451.0
76	12176.0
77	8901.0
78	7414.5
79	5928.0
80	4507.0
81	3086.0
82	3086.0
83	2322.0
84	1558.0
85	1266.5
86	975.0
87	821.0
88	667.0
89	667.0
90	503.5
91	340.0
92	190.0
93	40.0
94	25.5
95	11.0
96	11.0
97	6.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2010710.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.801748540288344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.31850935141287	47.89438765470905
2	9.730153830891213	11.053795023212121
3	2.506348970566226	4.2709501194094
4	1.0139432455462096	2.303749970705586
5	0.5251512859515078	1.4914755645113302
6	0.3564368162394982	1.2147740643922165
7	0.2493646195933715	0.9915042491891143
8	0.16556143757487052	0.7523343316077151
9	0.12237874330699389	0.6256193943600326
>10	0.7964951599383131	8.739121228271152
>50	0.10287136224433166	4.102020115416364
>100	0.10384056625115282	11.63866862285862
>500	0.007541534329204908	2.8668071464259777
>1k	0.0011399993753449277	0.9206518484795383
>5k	2.6307677892575257E-4	1.1341406664519076
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8699	0.4326332489518627	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7933	0.39453725301013076	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6137	0.30521557061933346	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4525	0.2250448846427381	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.973367616414102E-5	0.0
2	0.0	0.0	0.0	1.9893470465656409E-4	0.0
3	0.0	0.0	0.0	3.9786940931312817E-4	0.0
4	0.0	0.0	0.0	5.968041139696923E-4	0.0
5	0.0	0.0	0.0	5.968041139696923E-4	0.0
6	0.0	0.0	0.0	5.968041139696923E-4	0.0
7	0.0	0.0	0.0	6.465377901338333E-4	0.0
8	0.0	0.0	0.0	7.460051424621153E-4	0.0
9	0.0	0.0	0.0	9.449398471186795E-4	0.0
10	0.0	0.0	0.0	0.0016412113134166538	0.0
11	0.0	0.0	0.0	0.001790412341909077	0.0
12	0.0	0.0	0.0	0.002188281751222205	0.0
13	0.0	0.0	0.0	0.002287749103550487	0.0
14	0.0	0.0	0.0	0.002685618512863615	0.0
15	0.0	0.0	0.0	0.003580824683818154	0.0
16	0.0	0.0	0.0	0.005222035997234808	0.0
17	0.0	0.0	0.0	0.007062182015308026	0.0
18	0.0	0.0	0.0	0.008454724947903974	0.0
19	0.0	0.0	0.0	0.010444071994469615	0.0
20	0.0	0.0	0.0	0.011936082279393846	0.0
21	0.0	0.0	0.0	0.015168771230063013	0.0
22	0.0	0.0	0.0	0.020689209284282667	0.0
23	0.0	0.0	0.0	0.029989406726977037	0.0
24	0.0	0.0	0.0	0.043069363558146126	0.0
25	0.0	0.0	0.0	0.04729672603209811	0.0
26	0.0	0.0	0.0	0.05485624480904755	0.0
27	0.0	0.0	0.0	0.06883140781117118	0.0
28	0.0	0.0	0.0	0.1019540361364891	0.0
29	0.0	0.0	0.0	0.14746034982667813	0.0
30	0.0	0.0	0.0	0.21624202396168518	0.0
31	0.0	0.0	0.0	0.4522283173605343	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	0.0019316877	23.125002	5
ATACCGT	190	0.0	17.526316	6
CAAGACG	290	0.0	17.224138	4
GACGGAC	265	0.0	16.754715	7
GGTATCA	4235	0.0	16.599764	1
AGTCGGT	260	0.0	16.365385	11
CTTATAC	1015	0.0	15.857143	37
TAGCGCA	95	7.063884E-5	15.578948	4
TCTATAC	155	7.21775E-9	15.5161295	3
CAGTCGG	275	0.0	15.472727	10
CGGACCA	305	0.0	15.163935	9
CGCAAGA	320	0.0	15.031251	2
GCGCAAG	310	0.0	14.919355	1
CTACTAG	165	1.646913E-8	14.575757	1
TCTTATA	1800	0.0	14.491667	37
AAGACGG	335	0.0	14.358209	5
ACGGACC	310	0.0	14.322581	8
TACGTTA	155	1.2114288E-7	14.322581	19
CGAATTA	130	4.4476637E-6	14.230769	15
TTACACT	260	0.0	14.230769	4
>>END_MODULE