FastQCFastQC Report
Fri 10 Feb 2017
ERR1630780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502606
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC55740.37095552659845626No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT46940.3123906067192597No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA39110.26028113823583826No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA38750.2578853006044166No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG34480.22946800425394281No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG33060.22001775581889063No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG31270.20810511870709955No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG30570.20344654553489072No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG29780.1981890129548265No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG27910.1857439674804972No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG27610.18374743612097913No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG25740.17130239064664987No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA23880.1589238962176379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.15592909917836079No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA23220.15453152722669816No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA21320.14188682861641708No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA20580.13696205126293917No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT20150.13410035631429662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19850.13210382495477857No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA19280.12831041537169424No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC19220.12791110909979064No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC19160.12751180282788702No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG18850.1254487204230517No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA18410.12252047442909185No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC17840.11872706484600754No Hit
GTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTC16280.1083451017765136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16100.10714718296080278No Hit
TGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGT16040.10674787668889915No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG15810.10521720264660198No Hit
GGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTC15730.10468479428406383No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG15720.10461824323874655No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG15620.10395273278557386No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC15550.10348687546835299No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA15470.10295446710581484No Hit
GTAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAG15150.10082483365566222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15400.019.340911
GCTTTAT10300.019.2184451
TAATACT2200.018.54
TTTCGGA1205.176844E-918.530
AGTCTAT609.2386146E-418.51
CGAATTA609.2386146E-418.515
CTTTATT11150.017.7533632
CGGGTAA2400.017.72916824
GCGGGTA2400.017.72916823
TACGCTA852.7240183E-517.4117649
CGCTATA650.001580239217.0769232
CCAATAC1554.0199666E-1016.7096793
CAGTCGG1452.9813236E-916.58620610
GTAATAC904.4474185E-516.4444453
TTTATTG12000.016.3416673
ACCTAGG17850.015.649861
GTATCTA17450.015.58452826
CCTACAC1902.7284841E-1115.5789483
ACGCTAT957.061985E-515.57894810
GTACTAT1101.4523794E-515.1363641
Produced by FastQC (version 0.11.5)