##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630780.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1502606
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.946399788101473	33.0	31.0	34.0	30.0	34.0
2	32.12554788148057	33.0	31.0	34.0	30.0	34.0
3	32.2437006108055	34.0	31.0	34.0	30.0	34.0
4	35.83770595884749	37.0	35.0	37.0	35.0	37.0
5	35.79283192001097	37.0	35.0	37.0	35.0	37.0
6	35.86635085977296	37.0	35.0	37.0	35.0	37.0
7	35.81511720304591	37.0	35.0	37.0	35.0	37.0
8	35.80559308295055	37.0	35.0	37.0	35.0	37.0
9	37.51349122790672	39.0	37.0	39.0	35.0	39.0
10	37.41228971533456	39.0	37.0	39.0	34.0	39.0
11	37.504822954254145	39.0	37.0	39.0	35.0	39.0
12	37.44020987537651	39.0	37.0	39.0	35.0	39.0
13	37.49010918364495	39.0	37.0	39.0	35.0	39.0
14	38.76646439585627	40.0	38.0	41.0	35.0	41.0
15	38.78702999988021	40.0	38.0	41.0	35.0	41.0
16	38.744294246129726	40.0	38.0	41.0	35.0	41.0
17	38.73165487160307	40.0	38.0	41.0	35.0	41.0
18	38.70398560900196	40.0	38.0	41.0	35.0	41.0
19	38.737539980540475	40.0	38.0	41.0	35.0	41.0
20	38.71300726870517	40.0	38.0	41.0	34.0	41.0
21	38.65567088112253	40.0	38.0	41.0	34.0	41.0
22	38.59931878350013	40.0	38.0	41.0	34.0	41.0
23	38.56839450927256	40.0	38.0	41.0	34.0	41.0
24	38.50528681504	40.0	38.0	41.0	34.0	41.0
25	38.455537246623535	40.0	38.0	41.0	34.0	41.0
26	38.33204179938054	40.0	38.0	41.0	34.0	41.0
27	38.19972301454939	40.0	38.0	41.0	33.0	41.0
28	38.102593094929745	40.0	38.0	41.0	33.0	41.0
29	38.062416228871705	40.0	38.0	41.0	33.0	41.0
30	37.98726479196809	40.0	37.0	41.0	33.0	41.0
31	37.955954521677675	40.0	37.0	41.0	33.0	41.0
32	37.89727313746917	40.0	37.0	41.0	33.0	41.0
33	37.82481502136954	40.0	37.0	41.0	33.0	41.0
34	37.79302558355284	40.0	37.0	41.0	33.0	41.0
35	37.7399997071754	40.0	37.0	41.0	33.0	41.0
36	37.73108652567606	40.0	37.0	41.0	33.0	41.0
37	37.61256111049736	40.0	37.0	41.0	32.0	41.0
38	37.49375418439698	40.0	37.0	41.0	32.0	41.0
39	37.45181238461713	40.0	37.0	41.0	32.0	41.0
40	37.28722100138027	40.0	36.0	41.0	31.0	41.0
41	37.271340590946664	39.0	36.0	41.0	31.0	41.0
42	37.15630245054259	39.0	36.0	41.0	31.0	41.0
43	36.318601150268265	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	3.0
12	1.0
13	4.0
14	3.0
15	4.0
16	5.0
17	7.0
18	30.0
19	70.0
20	127.0
21	311.0
22	584.0
23	1057.0
24	1904.0
25	3142.0
26	4792.0
27	7464.0
28	10803.0
29	15310.0
30	21002.0
31	27828.0
32	35835.0
33	46938.0
34	62918.0
35	84536.0
36	120061.0
37	187182.0
38	365632.0
39	505043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.311345755307784	17.671099409958433	11.229690284745303	25.787864549988488
2	20.29573953518088	19.36875002495664	31.957146450899305	28.378363988963173
3	21.76012873634206	21.073388499713165	27.91363803951269	29.252844724432087
4	15.218094430609222	13.635843328191156	32.88533387993925	38.26072836126037
5	15.536541182452353	35.59928550797748	34.10934070541446	14.754832604155713
6	38.88524337051762	32.50559361535892	13.791639325278881	14.817523688844581
7	31.699261150294888	28.31853459922295	19.817836478757574	20.16436777172459
8	30.21550559494638	30.21703626898868	18.706367470913865	20.861090665151078
9	27.39866605084766	12.819661308420171	18.068009844230623	41.713662796501545
10	17.92865195533626	25.817479765154673	31.653607133207238	24.600261146301825
11	38.1386737441485	20.34864761620811	20.09036300933179	21.422315630311605
12	25.111506276429086	23.755195972863145	26.264236932369496	24.869060818338273
13	33.1922673009425	17.968582582526622	23.00117262941849	25.83797748711239
14	24.240153440090083	18.941758518201045	23.29765753630692	33.52043050540195
15	27.42282408029783	26.78000753357833	20.318899298951287	25.47826908717255
16	27.246330708116435	24.856815425999894	21.825614964934253	26.071238900949417
17	26.48092713592252	24.37285622445272	22.758327865055776	26.387888774568978
18	25.32214033485824	23.540502300669637	25.070311179377697	26.067046185094426
19	27.62607097269677	23.151511440790202	24.695029834833615	24.527387751679417
20	28.210056395355803	22.684056898481707	22.970825352753817	26.135061353408677
21	27.36439226250927	22.87911801230662	23.830997613479514	25.9254921117046
22	27.491371656974618	23.315227012270682	23.8297996946638	25.3636016360909
23	26.71997849070215	23.164488894627066	23.006563264089188	27.108969350581592
24	26.036898561565707	23.661425550011113	24.550813719631094	25.750862168792082
25	26.60937065338485	23.78527704534655	23.18977829184763	26.41557400942097
26	26.782270269119117	23.883439837189524	23.507892288464173	25.826397605227186
27	26.657353957058604	22.44414038011295	23.890693901129108	27.00781176169934
28	26.039094746061174	23.642125746869105	23.464833762143904	26.853945744925817
29	26.73395421021878	24.13739862612022	23.979206791401072	25.149440372259928
30	26.98159064984434	23.399946492959565	22.765914684221944	26.852548172974156
31	25.277418032405034	24.700819775776218	24.318550571473825	25.703211620344923
32	25.476006351631764	23.397750308464097	23.556341449455147	27.569901890448996
33	24.72976948048923	24.673800051377405	24.10791651304467	26.488513955088695
34	27.60524049551246	22.206686250420933	23.779952961721172	26.408120292345433
35	25.75731762018786	22.682060367122187	24.126284601552236	27.434337411137715
36	24.903534259812616	23.998173839316493	25.01540656699095	26.08288533387994
37	25.922830069891905	22.325612968402893	25.441000501794882	26.310556459910316
38	26.240744413372504	23.18245767686273	24.199224547219963	26.377573362544805
39	25.704409539160633	22.368272188451265	25.28620277038691	26.64111550200119
40	24.447260293117424	22.151648535943554	25.91956906867136	27.481522102267657
41	23.29253310581749	22.74774624885033	26.651630567161316	27.30809007817086
42	24.32014779656144	22.113647889067394	27.4161689757661	26.150035338605065
43	24.877180045866982	22.189116774457176	26.552469509638588	26.381233670037258
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45.0
1	46.5
2	48.0
3	179.5
4	311.0
5	311.0
6	436.5
7	562.0
8	575.5
9	589.0
10	797.0
11	1005.0
12	1005.0
13	1694.0
14	2383.0
15	3686.0
16	4989.0
17	4806.5
18	4624.0
19	4624.0
20	5520.5
21	6417.0
22	6675.5
23	6934.0
24	8699.5
25	10465.0
26	10465.0
27	12790.0
28	15115.0
29	18369.5
30	21624.0
31	25469.5
32	29315.0
33	29315.0
34	34032.0
35	38749.0
36	43900.0
37	49051.0
38	53739.0
39	58427.0
40	58427.0
41	62323.0
42	66219.0
43	69011.0
44	71803.0
45	77096.5
46	82390.0
47	82390.0
48	91011.0
49	99632.0
50	102563.0
51	105494.0
52	108559.0
53	111624.0
54	111624.0
55	107863.5
56	104103.0
57	106640.0
58	109177.0
59	114667.0
60	120157.0
61	120157.0
62	111496.0
63	102835.0
64	96688.0
65	90541.0
66	79332.5
67	68124.0
68	68124.0
69	60728.0
70	53332.0
71	44560.0
72	35788.0
73	25771.0
74	15754.0
75	15754.0
76	11157.0
77	6560.0
78	5158.5
79	3757.0
80	2954.0
81	2151.0
82	2151.0
83	1673.0
84	1195.0
85	938.0
86	681.0
87	541.5
88	402.0
89	402.0
90	295.0
91	188.0
92	111.0
93	34.0
94	19.5
95	5.0
96	5.0
97	4.0
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1502606.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.522168382120654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.09781966822739	46.94239821284553
2	8.709915876359041	9.497670000099072
3	2.1502188818715613	3.517037878074494
4	0.8852888136844483	1.9307146306604535
5	0.4782660023480067	1.3038049755730867
6	0.284832006599115	0.9317795174648551
7	0.1941057700901698	0.7408147236558206
8	0.14612141588301097	0.6373485152805922
9	0.11865235385016594	0.5822265253998338
>10	0.7448213781180854	7.938868969387361
>50	0.08561649458638113	3.2752009121194527
>100	0.08364458015360299	9.610853383767344
>500	0.01273646660256188	4.961762846772925
>1k	0.007837825601576543	7.757336617041917
>5k	1.224660250246335E-4	0.3721822918572505
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	5574	0.37095552659845626	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	4694	0.3123906067192597	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	3911	0.26028113823583826	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	3875	0.2578853006044166	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	3448	0.22946800425394281	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	3306	0.22001775581889063	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	3127	0.20810511870709955	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	3057	0.20344654553489072	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	2978	0.1981890129548265	No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG	2791	0.1857439674804972	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	2761	0.18374743612097913	No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG	2574	0.17130239064664987	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	2388	0.1589238962176379	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2343	0.15592909917836079	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	2322	0.15453152722669816	No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA	2132	0.14188682861641708	No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA	2058	0.13696205126293917	No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT	2015	0.13410035631429662	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1985	0.13210382495477857	No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA	1928	0.12831041537169424	No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC	1922	0.12791110909979064	No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC	1916	0.12751180282788702	No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG	1885	0.1254487204230517	No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA	1841	0.12252047442909185	No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC	1784	0.11872706484600754	No Hit
GTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTC	1628	0.1083451017765136	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1610	0.10714718296080278	No Hit
TGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGT	1604	0.10674787668889915	No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG	1581	0.10521720264660198	No Hit
GGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTC	1573	0.10468479428406383	No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG	1572	0.10461824323874655	No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG	1562	0.10395273278557386	No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC	1555	0.10348687546835299	No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA	1547	0.10295446710581484	No Hit
GTAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAG	1515	0.10082483365566222	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.65510453172688E-5	0.0
2	0.0	0.0	0.0	6.65510453172688E-4	0.0
3	0.0	6.65510453172688E-5	0.0	7.986125438072256E-4	0.0
4	0.0	6.65510453172688E-5	0.0	0.0017968782235662575	0.0
5	0.0	6.65510453172688E-5	0.0	0.001996531359518064	0.0
6	0.0	6.65510453172688E-5	0.0	0.0020630824048353327	0.0
7	0.0	6.65510453172688E-5	0.0	0.0023958376314216767	0.0
8	0.0	6.65510453172688E-5	0.0	0.0024623886767389455	0.0
9	0.0	6.65510453172688E-5	0.0	0.004725124217526085	0.0
10	0.0	6.65510453172688E-5	0.0	0.007254063939582299	0.0
11	0.0	6.65510453172688E-5	0.0	0.008119227528706793	0.0
12	0.0	6.65510453172688E-5	0.0	0.00885128902719675	0.0
13	0.0	6.65510453172688E-5	0.0	0.0093836973897349	0.0
14	0.0	6.65510453172688E-5	0.0	0.009849554706955782	0.0
15	0.0	6.65510453172688E-5	0.0	0.010115758888224857	0.0
16	0.0	6.65510453172688E-5	0.0	0.01217884129306019	0.0
17	6.65510453172688E-5	6.65510453172688E-5	0.0	0.013709515335357372	0.0
18	6.65510453172688E-5	1.331020906345376E-4	0.0	0.014175372652578254	0.0
19	6.65510453172688E-5	1.331020906345376E-4	0.0	0.01550639355892363	0.0
20	6.65510453172688E-5	1.331020906345376E-4	0.0	0.0177025780543935	0.0
21	6.65510453172688E-5	1.331020906345376E-4	0.0	0.01996531359518064	0.0
22	6.65510453172688E-5	1.331020906345376E-4	0.0	0.024690437812706724	0.0
23	6.65510453172688E-5	1.331020906345376E-4	0.0	0.03141209338975087	0.0
24	6.65510453172688E-5	1.996531359518064E-4	0.0	0.04026338241694762	0.0
25	6.65510453172688E-5	1.996531359518064E-4	0.0	0.04305852632027291	0.0
26	6.65510453172688E-5	1.996531359518064E-4	0.0	0.04718469112994358	0.0
27	6.65510453172688E-5	1.996531359518064E-4	0.0	0.05550357179460218	0.0
28	6.65510453172688E-5	2.662041812690752E-4	0.0	0.08465292964356591	0.0
29	6.65510453172688E-5	3.32755226586344E-4	0.0	0.13536482617532475	0.0
30	6.65510453172688E-5	3.32755226586344E-4	0.0	0.2003186464049791	0.0
31	6.65510453172688E-5	3.32755226586344E-4	0.0	0.4725789727979257	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1540	0.0	19.34091	1
GCTTTAT	1030	0.0	19.218445	1
TAATACT	220	0.0	18.5	4
TTTCGGA	120	5.176844E-9	18.5	30
AGTCTAT	60	9.2386146E-4	18.5	1
CGAATTA	60	9.2386146E-4	18.5	15
CTTTATT	1115	0.0	17.753363	2
CGGGTAA	240	0.0	17.729168	24
GCGGGTA	240	0.0	17.729168	23
TACGCTA	85	2.7240183E-5	17.411764	9
CGCTATA	65	0.0015802392	17.076923	2
CCAATAC	155	4.0199666E-10	16.709679	3
CAGTCGG	145	2.9813236E-9	16.586206	10
GTAATAC	90	4.4474185E-5	16.444445	3
TTTATTG	1200	0.0	16.341667	3
ACCTAGG	1785	0.0	15.64986	1
GTATCTA	1745	0.0	15.584528	26
CCTACAC	190	2.7284841E-11	15.578948	3
ACGCTAT	95	7.061985E-5	15.578948	10
GTACTAT	110	1.4523794E-5	15.136364	1
>>END_MODULE