FastQCFastQC Report
Fri 10 Feb 2017
ERR1630779.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630779.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638450
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6970.10917064766230715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4550.023.5824171
TCGCCAT1450.021.68965513
CCTTATA450.003823973220.5555552
TCGTTTA656.895988E-519.92307730
GTCGCCA1650.019.06060612
AGGTCGC1850.019.010
TAGACTC500.007031540418.55
CGCATCG801.614837E-518.513
AATAGCC609.2306844E-418.526
ATGCCGT1156.391747E-817.69565234
TAGGACA2550.017.4117644
CGTTTAT752.0654536E-417.26666631
AATAACG752.0654536E-417.2666662
TTAGGAC2600.017.0769233
CCTAGAC650.001578893717.0769233
GTAGAGC650.001578893717.0769233
CTTGCAC650.001578893717.0769233
GCTTAGG2000.016.6500021
GTATTAG904.4409775E-516.4444451
GGTCGCC2050.016.24390411
Produced by FastQC (version 0.11.5)