##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630779.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	638450
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.881838828412562	33.0	31.0	34.0	30.0	34.0
2	32.075152322029915	33.0	31.0	34.0	30.0	34.0
3	32.16938522985355	34.0	31.0	34.0	30.0	34.0
4	35.79018090688386	37.0	35.0	37.0	35.0	37.0
5	35.734391103453675	37.0	35.0	37.0	35.0	37.0
6	35.80861148093038	37.0	35.0	37.0	35.0	37.0
7	35.75711802020518	37.0	35.0	37.0	33.0	37.0
8	35.75643355000392	37.0	35.0	37.0	33.0	37.0
9	37.46148797869841	39.0	37.0	39.0	35.0	39.0
10	37.3406547106273	39.0	37.0	39.0	34.0	39.0
11	37.44408332680711	39.0	37.0	39.0	35.0	39.0
12	37.374131098754795	39.0	37.0	39.0	34.0	39.0
13	37.41714777977915	39.0	37.0	39.0	34.0	39.0
14	38.683401989192575	40.0	38.0	41.0	35.0	41.0
15	38.704141279661684	40.0	38.0	41.0	35.0	41.0
16	38.662996319210585	40.0	38.0	41.0	34.0	41.0
17	38.62180123737176	40.0	38.0	41.0	34.0	41.0
18	38.600914715326184	40.0	38.0	41.0	34.0	41.0
19	38.65822382332211	40.0	38.0	41.0	34.0	41.0
20	38.61994517973216	40.0	38.0	41.0	34.0	41.0
21	38.56480382175582	40.0	38.0	41.0	34.0	41.0
22	38.4973952541311	40.0	38.0	41.0	34.0	41.0
23	38.437490798026474	40.0	38.0	41.0	34.0	41.0
24	38.38034145195395	40.0	38.0	41.0	34.0	41.0
25	38.33094525804683	40.0	38.0	41.0	34.0	41.0
26	38.199801080742425	40.0	38.0	41.0	34.0	41.0
27	38.069017150912366	40.0	38.0	41.0	33.0	41.0
28	37.97890359464328	40.0	37.0	41.0	33.0	41.0
29	37.9226047458689	40.0	37.0	41.0	33.0	41.0
30	37.8407768815099	40.0	37.0	41.0	33.0	41.0
31	37.826368548829194	40.0	37.0	41.0	33.0	41.0
32	37.73726525178166	40.0	37.0	41.0	33.0	41.0
33	37.68895136659096	40.0	37.0	41.0	32.0	41.0
34	37.64097893335422	40.0	37.0	41.0	32.0	41.0
35	37.603527292661916	40.0	37.0	41.0	32.0	41.0
36	37.60221003994048	40.0	37.0	41.0	32.0	41.0
37	37.48838123580547	40.0	37.0	41.0	32.0	41.0
38	37.35948938836244	40.0	36.0	41.0	31.0	41.0
39	37.326078784556344	39.0	36.0	41.0	31.0	41.0
40	37.17582112929752	39.0	36.0	41.0	31.0	41.0
41	37.14623698018639	39.0	36.0	41.0	31.0	41.0
42	37.04968125929987	39.0	36.0	41.0	31.0	41.0
43	36.189480773748926	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	7.0
18	20.0
19	49.0
20	79.0
21	185.0
22	289.0
23	569.0
24	933.0
25	1451.0
26	2275.0
27	3443.0
28	4881.0
29	6811.0
30	9124.0
31	12302.0
32	15944.0
33	20926.0
34	27825.0
35	37672.0
36	53484.0
37	81037.0
38	159096.0
39	200043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.968439188660035	18.507322421489544	12.026626987234708	26.49761140261571
2	20.145508653770854	20.60098676482105	32.77547184587673	26.478032735531364
3	21.16735844623698	21.95066175894745	28.8594251703344	28.022554624481167
4	15.798574673036258	15.1051765995771	32.75808598950584	36.3381627378808
5	15.615318349126792	35.80687602788002	33.51319602161485	15.064609601378336
6	36.94055916673193	33.18912992403477	14.669903672957945	15.200407236275353
7	30.408176051374426	29.232359620957006	20.193280601456653	20.16618372621192
8	27.942986921450384	32.45970710314042	19.377085128044484	20.22022084736471
9	27.07150129219203	13.513509280288197	18.65596366199389	40.75902576552588
10	17.63787297360796	26.089748609914636	32.26141436291017	24.010964053567232
11	37.17926227582426	20.692928185449137	20.099929516798497	22.027880021928105
12	22.65455399796382	24.635601848226173	27.537473568799438	25.172370585010572
13	31.555799201190382	18.788002192810712	23.67217479833973	25.984023807659174
14	24.048868353042526	20.073772417573814	23.158743832719868	32.7186153966638
15	26.986138303704283	26.47458689012452	21.053645547811108	25.485629258360092
16	26.88605215756911	25.176129689090764	22.02036181376772	25.917456339572404
17	25.268854256402225	25.259769754875087	23.61860756519696	25.852768423525724
18	24.860051687681104	23.837888636541624	25.243010415850886	26.059049259926386
19	25.818936486803977	24.324692614926775	24.62354138930222	25.23282950896703
20	26.43793562534263	23.582269559088417	24.62354138930222	25.35625342626674
21	26.595817996710785	24.250293680006266	23.490954655807034	25.66293366747592
22	26.80303860913149	23.998120447959902	23.594956535359074	25.603884407549533
23	25.884094290860677	24.162581251468403	23.942986921450387	26.010337536220536
24	26.204871172370588	24.42947764116219	23.787140731459004	25.578510455008225
25	26.35476544756833	24.08035084971415	23.896781267131335	25.66810243558619
26	26.15725585402146	24.44106821207612	23.578197196334873	25.823478737567545
27	26.41271830213799	23.67577727308325	23.76568251233456	26.1458219124442
28	25.68564492129376	23.987626282402694	24.322656433550005	26.004072362753544
29	25.253504581408098	24.413188190148013	24.366042759808913	25.967264468634976
30	25.15341843527293	25.198684313571935	24.343331505991074	25.30456574516407
31	26.096796930065004	24.546166496984885	23.6431983710549	25.713838201895218
32	24.993813141201347	24.46409272456731	24.39501918709374	26.1470749471376
33	25.214660505912757	24.009084501527138	24.560576395958964	26.215678596601144
34	25.87986529877046	23.53856997415616	24.633409037512727	25.948155689560654
35	25.53590727543269	23.89004620565432	24.98629493304096	25.58775158587203
36	25.252721434724723	24.53191322734748	24.43214033988566	25.78322499804213
37	26.157882371368157	23.45602631372856	24.447333385543114	25.938757929360168
38	25.051296107760983	23.777116453911816	25.403085597932495	25.76850184039471
39	25.308794737254285	23.429555955830526	25.321011825514915	25.940637481400262
40	24.564492129375832	23.174720025060694	26.291487195551728	25.969300650011746
41	24.117315373169397	23.305818779857468	26.65940950740074	25.917456339572404
42	24.689795598715637	22.790978150207533	26.5690343801394	25.950191870937427
43	23.88910642963427	22.62918004542251	26.718928655337145	26.762784869606076
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	10.0
2	12.0
3	26.0
4	40.0
5	40.0
6	76.0
7	112.0
8	114.5
9	117.0
10	183.0
11	249.0
12	249.0
13	427.0
14	605.0
15	931.5
16	1258.0
17	1267.0
18	1276.0
19	1276.0
20	1648.0
21	2020.0
22	2321.5
23	2623.0
24	3275.0
25	3927.0
26	3927.0
27	4955.5
28	5984.0
29	8168.0
30	10352.0
31	11879.0
32	13406.0
33	13406.0
34	15511.5
35	17617.0
36	19956.0
37	22295.0
38	25742.0
39	29189.0
40	29189.0
41	31990.0
42	34791.0
43	35327.5
44	35864.0
45	38472.0
46	41080.0
47	41080.0
48	43437.0
49	45794.0
50	47464.0
51	49134.0
52	52831.0
53	56528.0
54	56528.0
55	53443.5
56	50359.0
57	48532.5
58	46706.0
59	43914.0
60	41122.0
61	41122.0
62	38824.5
63	36527.0
64	32401.5
65	28276.0
66	24760.5
67	21245.0
68	21245.0
69	17954.5
70	14664.0
71	12768.5
72	10873.0
73	8506.0
74	6139.0
75	6139.0
76	4835.0
77	3531.0
78	2924.0
79	2317.0
80	1774.5
81	1232.0
82	1232.0
83	919.0
84	606.0
85	460.5
86	315.0
87	240.5
88	166.0
89	166.0
90	122.5
91	79.0
92	44.5
93	10.0
94	5.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	638450.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.85013509881357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.88881440775741	61.88857015846278
2	6.414594079399833	8.832913379414562
3	1.4310295003609323	2.955797232907136
4	0.5866547780549046	1.615650429017787
5	0.3268827606865604	1.1252961117371414
6	0.2061971300440002	0.8518020156310224
7	0.1449757619561353	0.6987120557713347
8	0.10702255249425904	0.5894813758279682
9	0.08761990526589794	0.5429378083412087
>10	0.6446302876192913	9.170971568151156
>50	0.10414892479662648	4.884367727252708
>100	0.05629042919188087	6.392078610593098
>500	0.001139482372291089	0.4514215268921086
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	697	0.10917064766230715	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.1325867334951836E-4	0.0	3.1325867334951836E-4	0.0
3	0.0	3.1325867334951836E-4	0.0	6.265173466990367E-4	0.0
4	0.0	3.1325867334951836E-4	0.0	6.265173466990367E-4	0.0
5	0.0	3.1325867334951836E-4	0.0	6.265173466990367E-4	0.0
6	0.0	3.1325867334951836E-4	0.0	7.831466833737959E-4	0.0
7	0.0	3.1325867334951836E-4	0.0	9.397760200485551E-4	0.0
8	0.0	3.1325867334951836E-4	0.0	0.0010964053567233142	0.0
9	0.0	4.6988801002427753E-4	0.0	0.002349440050121388	0.0
10	0.0	4.6988801002427753E-4	0.0	0.003445845406844702	0.0
11	1.5662933667475918E-4	4.6988801002427753E-4	0.0	0.004385621426893257	0.0
12	1.5662933667475918E-4	4.6988801002427753E-4	0.0	0.004855509436917534	0.0
13	1.5662933667475918E-4	4.6988801002427753E-4	0.0	0.0053253974469418125	0.0
14	1.5662933667475918E-4	4.6988801002427753E-4	0.0	0.00563865612029133	0.0
15	1.5662933667475918E-4	4.6988801002427753E-4	0.0	0.005951914793640849	0.0
16	1.5662933667475918E-4	4.6988801002427753E-4	0.0	0.0064218028036651265	0.0
17	1.5662933667475918E-4	4.6988801002427753E-4	0.0	0.007048320150364163	0.0
18	1.5662933667475918E-4	6.265173466990367E-4	0.0	0.007361578823713681	0.0
19	3.1325867334951836E-4	6.265173466990367E-4	0.0	0.008457984180436995	0.0
20	3.1325867334951836E-4	6.265173466990367E-4	0.0	0.009711018873835069	0.0
21	3.1325867334951836E-4	6.265173466990367E-4	0.0	0.011747200250606939	0.0
22	3.1325867334951836E-4	6.265173466990367E-4	0.0	0.014096640300728326	0.0
23	3.1325867334951836E-4	6.265173466990367E-4	0.0	0.018012373717597306	0.0
24	3.1325867334951836E-4	6.265173466990367E-4	0.0	0.02490406453128671	0.0
25	3.1325867334951836E-4	6.265173466990367E-4	0.0	0.026783616571383822	0.0
26	3.1325867334951836E-4	6.265173466990367E-4	0.0	0.029602944631529484	0.0
27	3.1325867334951836E-4	6.265173466990367E-4	0.0	0.038374187485316	0.0
28	3.1325867334951836E-4	6.265173466990367E-4	0.0	0.05857937191635994	0.0
29	3.1325867334951836E-4	7.831466833737959E-4	0.0	0.09256793797478267	0.0
30	3.1325867334951836E-4	7.831466833737959E-4	0.0	0.1357976348970162	0.0
31	3.1325867334951836E-4	7.831466833737959E-4	0.0	0.297908998355392	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	455	0.0	23.582417	1
TCGCCAT	145	0.0	21.689655	13
CCTTATA	45	0.0038239732	20.555555	2
TCGTTTA	65	6.895988E-5	19.923077	30
GTCGCCA	165	0.0	19.060606	12
AGGTCGC	185	0.0	19.0	10
TAGACTC	50	0.0070315404	18.5	5
CGCATCG	80	1.614837E-5	18.5	13
AATAGCC	60	9.2306844E-4	18.5	26
ATGCCGT	115	6.391747E-8	17.695652	34
TAGGACA	255	0.0	17.411764	4
CGTTTAT	75	2.0654536E-4	17.266666	31
AATAACG	75	2.0654536E-4	17.266666	2
TTAGGAC	260	0.0	17.076923	3
CCTAGAC	65	0.0015788937	17.076923	3
GTAGAGC	65	0.0015788937	17.076923	3
CTTGCAC	65	0.0015788937	17.076923	3
GCTTAGG	200	0.0	16.650002	1
GTATTAG	90	4.4409775E-5	16.444445	1
GGTCGCC	205	0.0	16.243904	11
>>END_MODULE