FastQCFastQC Report
Fri 10 Feb 2017
ERR1630778.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630778.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38801
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT11522.9689956444421535No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT9162.3607638978376846TruSeq Adapter, Index 7 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6771.744800391742481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6251.6107832272364113No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC5841.5051158475297028No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC5701.4690343032396072No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG5491.4149119868044637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4901.2628540501533467No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.5412231643514342No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC1930.4974098605706039RNA PCR Primer, Index 7 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG1690.43555578464472566No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTC1520.3917424808638953No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1140.29380686064792144No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.17009870879616504No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT660.17009870879616504No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA570.14690343032396072No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA560.14432617716038246No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA480.1237081518517564No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA470.12113089868817813No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.10824463287028685No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT410.10566737970670859No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.10309012654313034No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA400.10309012654313034No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT400.10309012654313034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGG554.6929927E-1033.63636410
CCGTCTT1100.033.63636437
GCAGTCG554.6929927E-1033.6363649
GCCGTCT1150.032.17391236
TTCCTCC303.5363293E-430.83333220
AGTCGGT601.1914381E-930.83333211
CGGTGAT601.1914381E-930.83333214
GTGATTC601.1914381E-930.83333216
GATTCCT601.1914381E-930.83333218
GGCAGTC601.1914381E-930.8333328
ATTCCTC551.7600541E-830.27272819
CGCCTTA250.005431946429.59999825
TCGCCTT250.005431946429.59999824
CTCGCCT250.005431946429.59999823
GGTGATT652.8030627E-928.46153815
GGGCAGT652.8030627E-928.4615387
TGATTCC652.8030627E-928.46153817
TTATTGA556.003902E-726.9090929
TATTGAT556.003902E-726.9090930
ATTGATA556.003902E-726.9090931
Produced by FastQC (version 0.11.5)