##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630778.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38801 Sequences flagged as poor quality 0 Sequence length 43 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.834463029303368 33.0 31.0 34.0 30.0 34.0 2 31.994587768356485 33.0 31.0 34.0 30.0 34.0 3 31.986211695574855 33.0 31.0 34.0 30.0 34.0 4 35.701992216695444 37.0 35.0 37.0 33.0 37.0 5 35.69222442720548 37.0 35.0 37.0 35.0 37.0 6 35.775572794515604 37.0 35.0 37.0 35.0 37.0 7 35.71645060694312 37.0 35.0 37.0 33.0 37.0 8 35.756088760598956 37.0 35.0 37.0 35.0 37.0 9 37.37401097909848 39.0 37.0 39.0 34.0 39.0 10 37.31362593747584 39.0 37.0 39.0 34.0 39.0 11 37.39535063529291 39.0 37.0 39.0 34.0 39.0 12 37.34746527151362 39.0 37.0 39.0 34.0 39.0 13 37.40408752351743 39.0 37.0 39.0 34.0 39.0 14 38.47127651349192 40.0 38.0 41.0 34.0 41.0 15 38.43011778046957 40.0 38.0 41.0 34.0 41.0 16 38.30514677456767 40.0 38.0 41.0 34.0 41.0 17 38.3700677817582 40.0 38.0 41.0 34.0 41.0 18 38.5370222416948 40.0 38.0 41.0 34.0 41.0 19 38.59160846369939 40.0 38.0 41.0 34.0 41.0 20 38.61949434292931 40.0 38.0 41.0 34.0 41.0 21 38.59361872116698 40.0 38.0 41.0 34.0 41.0 22 38.40519574237777 40.0 38.0 41.0 34.0 41.0 23 38.403855570732716 40.0 38.0 41.0 34.0 41.0 24 38.25996752661014 40.0 38.0 41.0 34.0 41.0 25 38.178887142083966 40.0 37.0 41.0 33.0 41.0 26 38.015180021133474 40.0 37.0 41.0 33.0 41.0 27 37.72366691580114 40.0 37.0 41.0 33.0 41.0 28 37.663848869874485 40.0 37.0 41.0 32.0 41.0 29 37.50686837968094 40.0 36.0 41.0 32.0 41.0 30 37.36035153733151 39.0 36.0 41.0 32.0 41.0 31 37.176258343857114 39.0 35.0 41.0 32.0 41.0 32 36.853663565372024 39.0 35.0 41.0 31.0 41.0 33 36.69340996366073 39.0 35.0 41.0 31.0 41.0 34 36.55511455890312 39.0 35.0 41.0 31.0 41.0 35 36.42421587072498 39.0 35.0 41.0 30.0 41.0 36 36.31055900621118 39.0 35.0 41.0 30.0 41.0 37 36.04956057833561 38.0 35.0 41.0 30.0 41.0 38 35.796371227545684 38.0 35.0 40.0 29.0 41.0 39 35.59885054508904 38.0 35.0 40.0 27.0 41.0 40 35.20267518878379 38.0 34.0 40.0 26.0 41.0 41 35.05744697301616 38.0 34.0 40.0 26.0 41.0 42 34.6920182469524 38.0 34.0 40.0 23.0 41.0 43 33.70526532821319 36.0 33.0 40.0 21.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 2.0 13 0.0 14 1.0 15 1.0 16 1.0 17 0.0 18 1.0 19 5.0 20 10.0 21 21.0 22 31.0 23 60.0 24 111.0 25 154.0 26 239.0 27 309.0 28 476.0 29 624.0 30 810.0 31 1018.0 32 1196.0 33 1564.0 34 1988.0 35 2594.0 36 3572.0 37 6076.0 38 8142.0 39 9793.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.547872477513465 17.347491044045256 15.587227133321308 24.51740934511997 2 18.12582149944589 20.752042473132136 34.8625035437231 26.259632483698876 3 19.710832195046518 24.422050978067574 35.14342413855313 20.723692688332775 4 11.783201463879797 18.566531790417773 28.73895002706116 40.911316718641274 5 12.25226153965104 36.87533826447772 33.51202288600809 17.360377309863146 6 30.457977887167857 31.195072291951238 17.022757145434397 21.32419267544651 7 23.965877168114226 33.76717094920234 17.7572742970542 24.509677585629237 8 32.89348212674931 28.633282647354452 19.151568258550036 19.3216669673462 9 25.226153965103993 11.254864565346253 16.88616272776475 46.632818741785 10 20.72626994149635 26.80858740754104 23.344759155691865 29.12038349527074 11 31.39867529187392 21.267493105847787 18.06654467668359 29.2672869255947 12 21.290688384319992 30.934769722429834 21.937578928378134 25.83696296487204 13 34.8856988221953 15.734130563645266 25.787995154764054 23.592175459395374 14 24.96585139558259 21.55872271333213 23.36279992783691 30.11262596324837 15 30.476018659312903 21.87314759928868 21.342233447591557 26.30860029380686 16 27.56629983763305 21.968505966341073 24.334424370505914 26.130769825519963 17 18.90930646117368 33.0610035823819 22.795804231849694 25.233885724594728 18 24.589572433700162 18.906729208010102 26.883327749284813 29.62037060900492 19 22.138604675137238 26.811164660704623 29.13584701425221 21.91438364990593 20 23.976186180768536 21.87314759928868 29.05595216618128 25.094714053761503 21 31.69505940568542 18.612922347362183 26.228705445735933 23.463312801216464 22 25.18234066132316 26.409113167186415 24.022576737712946 24.38596943377748 23 27.70031700213912 20.721115435169196 24.806061699440736 26.772505863250945 24 23.46846730754362 24.03030849720368 28.26988995128992 24.231334243962785 25 21.955619700523183 25.58696940800495 28.311126001907166 24.1462848895647 26 27.045694698590246 20.615448055462487 26.81631916703178 25.522538078915492 27 20.76492873895003 28.01216463493209 27.57403159712379 23.648875028994098 28 23.584443699904643 20.9221411819283 29.612638849514187 25.880776268652873 29 19.963403005077186 23.02517976340816 31.792995025901394 25.218422205613255 30 21.386046751372387 24.661735522280352 32.91667740522152 21.035540321125744 31 23.5303213834695 22.200458751063117 26.870441483466923 27.398778382000465 32 20.476276384629262 24.452978016030517 31.43475683616402 23.635988763176208 33 22.499420118038195 22.259735573825417 31.968248241024718 23.27259606711167 34 20.77266049844076 26.566325610164686 26.553439344346796 26.107574547047758 35 19.651555372284218 23.579289193577484 35.834127986392105 20.935027447746194 36 20.71853818200562 24.525141104610707 25.932321331924435 28.82399938145924 37 19.643823612793483 26.599829901291205 29.692533697585116 24.063812788330196 38 20.04587510631169 22.264890080152576 31.215690317259863 26.47354449627587 39 22.321589649751296 23.73392438339218 28.086904976675857 25.85758099018067 40 18.70312620808742 21.759748460091235 36.04546274580552 23.491662586015824 41 20.409267802376228 24.39885569959537 27.780211850210044 27.411664647818355 42 19.81134506842607 22.57158320661839 34.22592201231927 23.39114971263627 43 18.063967423520015 23.780314940336588 32.566170974974874 25.589546661168526 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 2.5 2 4.0 3 28.5 4 53.0 5 53.0 6 82.0 7 111.0 8 118.0 9 125.0 10 154.0 11 183.0 12 183.0 13 350.5 14 518.0 15 899.0 16 1280.0 17 1140.0 18 1000.0 19 1000.0 20 1057.0 21 1114.0 22 773.0 23 432.0 24 389.0 25 346.0 26 346.0 27 386.0 28 426.0 29 455.5 30 485.0 31 506.5 32 528.0 33 528.0 34 548.0 35 568.0 36 603.0 37 638.0 38 747.0 39 856.0 40 856.0 41 1065.0 42 1274.0 43 1425.5 44 1577.0 45 2004.5 46 2432.0 47 2432.0 48 2832.5 49 3233.0 50 3009.5 51 2786.0 52 3311.5 53 3837.0 54 3837.0 55 2818.0 56 1799.0 57 2526.5 58 3254.0 59 2900.5 60 2547.0 61 2547.0 62 2048.5 63 1550.0 64 1481.5 65 1413.0 66 1366.5 67 1320.0 68 1320.0 69 1187.0 70 1054.0 71 941.5 72 829.0 73 693.5 74 558.0 75 558.0 76 446.0 77 334.0 78 267.5 79 201.0 80 143.0 81 85.0 82 85.0 83 57.5 84 30.0 85 22.5 86 15.0 87 9.5 88 4.0 89 4.0 90 2.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 38801.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.78441277286669 #Duplication Level Percentage of deduplicated Percentage of total 1 88.52289453793213 57.349037395943405 2 7.077216851652941 9.169866756011443 3 1.6748219755738554 3.25507074559934 4 0.8712256832557586 2.257673771294554 5 0.5291005291005291 1.7138733537795419 6 0.2824521621514103 1.097909847684338 7 0.23073556908143372 1.0463647844127728 8 0.1551497792099296 0.8041029870364166 9 0.10343318613995306 0.6030772402773124 >10 0.4853403349643951 5.574598592819773 >50 0.015912797867685086 0.6314270250766734 >100 0.023869196801527628 3.422592201231927 >500 0.023869196801527628 10.10540965439035 >1k 0.003978199466921272 2.9689956444421535 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT 1152 2.9689956444421535 No Hit TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT 916 2.3607638978376846 TruSeq Adapter, Index 7 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 677 1.744800391742481 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 625 1.6107832272364113 No Hit ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC 584 1.5051158475297028 No Hit CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC 570 1.4690343032396072 No Hit ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG 549 1.4149119868044637 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 490 1.2628540501533467 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 210 0.5412231643514342 No Hit CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC 193 0.4974098605706039 RNA PCR Primer, Index 7 (95% over 22bp) CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG 169 0.43555578464472566 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTC 152 0.3917424808638953 No Hit CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA 114 0.29380686064792144 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66 0.17009870879616504 No Hit TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT 66 0.17009870879616504 No Hit GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA 57 0.14690343032396072 No Hit CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA 56 0.14432617716038246 No Hit TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA 48 0.1237081518517564 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.12113089868817813 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42 0.10824463287028685 No Hit ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT 41 0.10566737970670859 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.10309012654313034 No Hit CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA 40 0.10309012654313034 No Hit CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT 40 0.10309012654313034 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.020618025308626067 0.0 3 0.0 0.0 0.0 0.036081544290095614 0.0 4 0.0 0.0 0.0 0.07216308858019123 0.0 5 0.0 0.0 0.0 0.08762660756166078 0.0 6 0.0 0.0 0.0 0.08762660756166078 0.0 7 0.0 0.0 0.0 0.10051287337955207 0.0 8 0.0 0.0 0.0 0.10824463287028685 0.0 9 0.0 0.0 0.0 0.17009870879616504 0.0 10 0.0 0.0 0.0 0.23968454421277802 0.0 11 0.0 0.0 0.0 0.2654570758485606 0.0 12 0.0 0.0 0.0 0.28607510115718665 0.0 13 0.0 0.0 0.0 0.2912296074843432 0.0 14 0.0 0.0 0.0 0.30153862013865623 0.0 15 0.0 0.0 0.0 0.3221566454472823 0.0 16 0.0 0.0 0.0 0.3350429112651736 0.0 17 0.0 0.0 0.0 0.35050643024664313 0.0 18 0.0 0.0 0.0 0.3633926960645344 0.0 19 0.0 0.0 0.0 0.3943197340274735 0.0 20 0.0025772531635782584 0.0 0.0 0.4200922656632561 0.0 21 0.0025772531635782584 0.0 0.0 0.4510193036261952 0.0 22 0.0025772531635782584 0.0 0.0 0.5412231643514342 0.0 23 0.0025772531635782584 0.0 0.0 0.639158784567408 0.0 24 0.0025772531635782584 0.0 0.0 0.7989484807092601 0.0 25 0.0025772531635782584 0.0 0.0 0.8350300249993556 0.0 26 0.0025772531635782584 0.0 0.0 0.8943068477616556 0.0 27 0.0025772531635782584 0.0 0.0 0.927811138888173 0.0 28 0.0025772531635782584 0.0 0.0 0.9948197211412076 0.0 29 0.0025772531635782584 0.0 0.0 1.0953325945207597 0.0 30 0.0025772531635782584 0.0 0.0 1.3298626324063814 0.0 31 0.0025772531635782584 0.0 0.0 1.925208113192959 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTCGG 55 4.6929927E-10 33.636364 10 CCGTCTT 110 0.0 33.636364 37 GCAGTCG 55 4.6929927E-10 33.636364 9 GCCGTCT 115 0.0 32.173912 36 TTCCTCC 30 3.5363293E-4 30.833332 20 AGTCGGT 60 1.1914381E-9 30.833332 11 CGGTGAT 60 1.1914381E-9 30.833332 14 GTGATTC 60 1.1914381E-9 30.833332 16 GATTCCT 60 1.1914381E-9 30.833332 18 GGCAGTC 60 1.1914381E-9 30.833332 8 ATTCCTC 55 1.7600541E-8 30.272728 19 CGCCTTA 25 0.0054319464 29.599998 25 TCGCCTT 25 0.0054319464 29.599998 24 CTCGCCT 25 0.0054319464 29.599998 23 GGTGATT 65 2.8030627E-9 28.461538 15 GGGCAGT 65 2.8030627E-9 28.461538 7 TGATTCC 65 2.8030627E-9 28.461538 17 TTATTGA 55 6.003902E-7 26.90909 29 TATTGAT 55 6.003902E-7 26.90909 30 ATTGATA 55 6.003902E-7 26.90909 31 >>END_MODULE