FastQCFastQC Report
Fri 10 Feb 2017
ERR1630777.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630777.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098037
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68080.6200155368170653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66970.609906587847222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54970.5006206530381034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35550.3237595818720134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACT450.003825700820.5555554
CGAACGA1101.7553248E-920.18181816
GCATAAG759.265152E-619.7333341
CCTATAC1153.0504452E-919.3043483
GCCGTCT1355.638867E-1119.18518636
GTCGGTT609.236456E-418.512
CTCGCAT500.00703468818.49999813
ACGAACG1258.576535E-917.7615
GTATAGG752.06716E-417.2666661
AAGACGG1201.041044E-716.9583345
GGTATCA30800.016.938311
CCGATAA1655.4569682E-1116.8181829
TAACGAA1352.2188033E-816.44444513
TTATACC1352.2188033E-816.4444454
GGTCGGT700.00259239415.85714311
TGCCGTC1751.3096724E-1015.85714335
TCCGATA1659.731593E-1015.696978
CAAGACG1659.731593E-1015.696974
GACGGAC1302.5884583E-715.6538457
CTAGACA1201.93521E-615.4166674
Produced by FastQC (version 0.11.5)