##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630777.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1098037
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.494063496949558	31.0	31.0	34.0	30.0	34.0
2	31.685814776733388	31.0	31.0	34.0	30.0	34.0
3	31.765578937686072	31.0	31.0	34.0	30.0	34.0
4	35.48264220604588	37.0	35.0	37.0	33.0	37.0
5	35.35219122852873	37.0	35.0	37.0	33.0	37.0
6	35.43164483528333	37.0	35.0	37.0	33.0	37.0
7	35.3934594189449	37.0	35.0	37.0	33.0	37.0
8	35.395870084523565	37.0	35.0	37.0	33.0	37.0
9	36.99167787606429	39.0	37.0	39.0	33.0	39.0
10	36.820308423122356	38.0	37.0	39.0	33.0	39.0
11	36.96065797418484	39.0	37.0	39.0	33.0	39.0
12	36.886085805851714	39.0	37.0	39.0	33.0	39.0
13	36.95404526441276	39.0	37.0	39.0	33.0	39.0
14	38.079712250133646	40.0	37.0	41.0	33.0	41.0
15	38.06513897072685	40.0	37.0	41.0	33.0	41.0
16	38.0161451754358	40.0	37.0	41.0	33.0	41.0
17	38.002056397006655	40.0	37.0	41.0	33.0	41.0
18	37.99189553721778	40.0	37.0	41.0	33.0	41.0
19	38.06140685605312	40.0	37.0	41.0	33.0	41.0
20	38.03303804880892	40.0	37.0	41.0	33.0	41.0
21	37.98242864311494	40.0	37.0	41.0	33.0	41.0
22	37.896700202270054	40.0	37.0	41.0	33.0	41.0
23	37.81802161493647	40.0	37.0	41.0	33.0	41.0
24	37.741168102714205	39.0	37.0	41.0	33.0	41.0
25	37.69149582391122	39.0	37.0	41.0	32.0	41.0
26	37.504715232728955	39.0	37.0	41.0	32.0	41.0
27	37.33988198940473	39.0	36.0	40.0	32.0	41.0
28	37.23440284799146	39.0	36.0	40.0	32.0	41.0
29	37.131594836968155	39.0	36.0	40.0	31.0	41.0
30	37.004759402460934	39.0	36.0	40.0	31.0	41.0
31	36.9276071753502	39.0	36.0	40.0	31.0	41.0
32	36.79219188424434	39.0	35.0	40.0	31.0	41.0
33	36.68111912440109	39.0	35.0	40.0	30.0	41.0
34	36.59473861081184	39.0	35.0	40.0	30.0	41.0
35	36.519110922491684	39.0	35.0	40.0	30.0	41.0
36	36.47336383018059	39.0	35.0	40.0	30.0	41.0
37	36.29982960501331	38.0	35.0	40.0	30.0	41.0
38	36.11882386476958	38.0	35.0	40.0	30.0	41.0
39	36.024594799628794	38.0	35.0	40.0	29.0	41.0
40	35.79818530705249	38.0	35.0	40.0	28.0	41.0
41	35.69659401277006	38.0	35.0	40.0	28.0	41.0
42	35.524865737675505	38.0	35.0	40.0	27.0	41.0
43	34.70191623779527	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	6.0
12	1.0
13	1.0
14	3.0
15	8.0
16	12.0
17	31.0
18	88.0
19	160.0
20	301.0
21	592.0
22	1028.0
23	1707.0
24	2852.0
25	4288.0
26	6262.0
27	9203.0
28	12981.0
29	17549.0
30	22571.0
31	29434.0
32	36754.0
33	47113.0
34	61074.0
35	81979.0
36	117631.0
37	184527.0
38	288682.0
39	171191.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.962850978610014	20.595845130901782	12.909218906102435	23.532084984385772
2	18.723230637947538	22.48257572376887	34.54264291640446	24.251550721879138
3	21.04792461456217	24.744066001418897	29.74571895118288	24.46229043283605
4	14.729376150348303	16.297902529696177	34.5663215356131	34.406399784342426
5	13.653182907315509	38.398432839694834	33.95705244905226	13.99133180393739
6	33.62190891563763	35.101094043279055	16.18005586332701	15.096941177756303
7	27.574571712975064	31.749294422683388	22.099619593875254	18.576514270466298
8	27.379223104503765	33.153618684980565	20.684184594872487	18.78297361564319
9	26.68798956683609	13.769754571111903	21.156755191309582	38.385500670742424
10	17.07219337781878	27.82765972367051	32.96291472873865	22.137232169772055
11	33.92781846149082	22.567272323245938	21.998439032564477	21.506470182698763
12	21.097558643287975	27.073313558650575	29.67195094518673	22.15717685287472
13	29.620586555826446	20.70431142119983	26.322974544573636	23.35212747840009
14	22.59778131338015	22.092607079725	26.912663234481172	28.39694837241368
15	25.299420693473902	27.984120753672237	24.179057718455756	22.537400834398113
16	23.89628036213716	27.065663543213937	25.716801892832393	23.32125420181651
17	22.12876250982435	27.367201651674762	27.237788890538294	23.26624694796259
18	21.960097883768945	25.396776247066356	29.164408849610712	23.478717019553986
19	22.558165162011846	26.294742344747945	29.272328710234717	21.87476378300549
20	23.512959945794176	25.249149163461702	29.08790869524433	22.14998219549979
21	23.925332206473918	25.86069504033106	27.96317428283382	22.2507984703612
22	23.59164581885674	25.85850932163488	28.335383962471212	22.214460897037167
23	22.94458201317442	25.903771912968327	28.37527332867654	22.776372745180716
24	22.769178087805784	26.44865336960412	28.430735940592168	22.351432601997928
25	22.86134255949481	26.153308130782477	28.285385647296042	22.699963662426676
26	22.57738127221578	26.58216435329593	28.302962468477837	22.537491906010455
27	22.89194262124136	26.189372489269484	28.17209256154392	22.746592327945233
28	22.270469938626842	26.302756646633945	28.80458490925169	22.62218850548752
29	21.854728028290484	26.74481825293683	29.041006814888753	22.35944690388393
30	22.000260464811294	27.36064449558621	28.602041643405457	22.03705339619703
31	22.443232787237587	27.158192301352322	28.057342329994345	22.341232581415746
32	21.745533165093708	26.975775861833434	28.395855513065587	22.882835460007268
33	21.69690092410365	26.669866315980244	28.97452453787987	22.658708222036232
34	22.392141612714326	26.26286728042862	28.737191916119404	22.607799190737655
35	22.07402847080745	26.553750010245558	28.962776299887892	22.4094452190591
36	21.849081588325348	26.95710618130354	28.42326806838021	22.770544161990898
37	22.36418262772566	26.3122280943174	28.461973503625103	22.86161577433183
38	21.83168691036823	26.263504781715007	29.08599619138517	22.818812116531593
39	21.99880331901384	26.031363241857967	29.154573115477895	22.815260323650296
40	21.342996638546786	25.878545076349884	29.886697807086648	22.89176047801668
41	21.02379063729182	26.169427806166823	29.867026338821006	22.93975521772035
42	21.275239358965134	26.00804890909869	29.90063176377481	22.816079968161365
43	20.876254625299513	25.496772877416703	29.794988693459327	23.83198380382446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	253.0
1	252.0
2	251.0
3	602.0
4	953.0
5	953.0
6	1435.5
7	1918.0
8	2008.0
9	2098.0
10	3080.5
11	4063.0
12	4063.0
13	6929.0
14	9795.0
15	13693.5
16	17592.0
17	17039.5
18	16487.0
19	16487.0
20	18184.5
21	19882.0
22	18279.0
23	16676.0
24	18337.0
25	19998.0
26	19998.0
27	22568.5
28	25139.0
29	29319.0
30	33499.0
31	36716.5
32	39934.0
33	39934.0
34	43086.0
35	46238.0
36	49478.5
37	52719.0
38	56020.5
39	59322.0
40	59322.0
41	61474.0
42	63626.0
43	63651.0
44	63676.0
45	64673.0
46	65670.0
47	65670.0
48	67225.5
49	68781.0
50	69007.5
51	69234.0
52	70297.5
53	71361.0
54	71361.0
55	69330.0
56	67299.0
57	64594.5
58	61890.0
59	57180.5
60	52471.0
61	52471.0
62	48076.5
63	43682.0
64	38699.5
65	33717.0
66	28938.0
67	24159.0
68	24159.0
69	20475.5
70	16792.0
71	14514.5
72	12237.0
73	9556.0
74	6875.0
75	6875.0
76	5384.5
77	3894.0
78	3155.5
79	2417.0
80	1975.0
81	1533.0
82	1533.0
83	1147.0
84	761.0
85	637.0
86	513.0
87	460.0
88	407.0
89	407.0
90	301.5
91	196.0
92	106.5
93	17.0
94	13.0
95	9.0
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1098037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.84937028240915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1450090337298	60.48432726758243
2	7.20258322569523	9.773814725401293
3	1.4473450465716675	2.946043499737554
4	0.5930022874893568	1.6093932712872405
5	0.30941154062114307	1.049668909462731
6	0.2077559080522929	0.8457664520278904
7	0.14517273777841985	0.6894915188307369
8	0.11066231362225165	0.6006694634611394
9	0.07842951386936872	0.4789253814832929
>10	0.6217023495194722	8.694147487982656
>50	0.08501594850175305	4.013633763290142
>100	0.05189350876912295	6.06623474711456
>500	0.0013443905200952657	0.5936340547152948
>1k	2.688781040190531E-4	0.4209620716827114
>5k	4.0331715602857964E-4	1.733287385940386
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6808	0.6200155368170653	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6697	0.609906587847222	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5497	0.5006206530381034	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3555	0.3237595818720134	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0010017877357502525	0.0
3	0.0	0.0	0.0	0.0016392890221367767	0.0
4	0.0	0.0	0.0	0.0030964348195916896	0.0
5	0.0	0.0	0.0	0.003733936105978214	0.0
6	0.0	0.0	0.0	0.003733936105978214	0.0
7	0.0	0.0	0.0	0.004098222555341942	0.0
8	0.0	0.0	0.0	0.004553580617046602	0.0
9	0.0	0.0	0.0	0.00755894382429736	0.0
10	0.0	0.0	0.0	0.011930381216662098	0.0
11	0.0	0.0	0.0	0.014116099912844467	0.0
12	0.0	0.0	0.0	0.016392890221367768	0.0
13	0.0	0.0	0.0	0.017303606344777088	0.0
14	0.0	0.0	0.0	0.019033966979254798	0.0
15	0.0	0.0	0.0	0.021492900512459962	0.0
16	0.0	0.0	0.0	0.02495362178141538	0.0
17	0.0	0.0	0.0	0.02941613078612105	0.0
18	0.0	0.0	0.0	0.03178399270698529	0.0
19	0.0	0.0	0.0	0.03469828430189511	0.0
20	0.0	0.0	0.0	0.03697507461041841	0.0
21	0.0	0.0	0.0	0.043532230698965516	0.0
22	0.0	0.0	0.0	0.05282153515774059	0.0
23	0.0	0.0	0.0	0.06766620796931251	0.0
24	0.0	0.0	0.0	0.08861267880772689	0.0
25	0.0	0.0	0.0	0.09644483746904703	0.0
26	0.0	0.0	0.0	0.10837521868570914	0.0
27	0.0	0.0	0.0	0.12485918051941783	0.0
28	0.0	0.0	0.0	0.1611056822311088	0.0
29	0.0	0.0	0.0	0.211741498692667	0.0
30	0.0	0.0	0.0	0.29261309045141465	0.0
31	0.0	0.0	0.0	0.5674672164963476	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACT	45	0.0038257008	20.555555	4
CGAACGA	110	1.7553248E-9	20.181818	16
GCATAAG	75	9.265152E-6	19.733334	1
CCTATAC	115	3.0504452E-9	19.304348	3
GCCGTCT	135	5.638867E-11	19.185186	36
GTCGGTT	60	9.236456E-4	18.5	12
CTCGCAT	50	0.007034688	18.499998	13
ACGAACG	125	8.576535E-9	17.76	15
GTATAGG	75	2.06716E-4	17.266666	1
AAGACGG	120	1.041044E-7	16.958334	5
GGTATCA	3080	0.0	16.93831	1
CCGATAA	165	5.4569682E-11	16.818182	9
TAACGAA	135	2.2188033E-8	16.444445	13
TTATACC	135	2.2188033E-8	16.444445	4
GGTCGGT	70	0.002592394	15.857143	11
TGCCGTC	175	1.3096724E-10	15.857143	35
TCCGATA	165	9.731593E-10	15.69697	8
CAAGACG	165	9.731593E-10	15.69697	4
GACGGAC	130	2.5884583E-7	15.653845	7
CTAGACA	120	1.93521E-6	15.416667	4
>>END_MODULE