FastQCFastQC Report
Fri 10 Feb 2017
ERR1630776.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630776.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068733
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76300.3688247830918731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72210.349054227877643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54320.2625761758525629No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37160.17962685373124515No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG33040.1597112822196001No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT26550.12833942321217867No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG25020.12094359204401922No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA24340.11765655596928169No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA21800.10537850945482088No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC21800.10537850945482088No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC21410.1034932975884273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA957.1540853E-921.42105315
GTACCGT555.1450066E-420.1818186
AATTCGC555.1450066E-420.18181829
CTAGCGG1600.019.6562529
TCTAGCG1700.019.58823628
ATTCGCG609.240221E-418.530
GGTATCA35300.017.8711051
TATACTG2800.017.8392875
TACGCTA1201.0423537E-716.9583329
CGCAATA1951.8189894E-1216.12820636
CGACGTC700.002593433315.85714334
CGGTCGA700.002593433315.85714320
CGCGCAA957.064042E-515.57894821
AGTACTA1201.9372237E-615.4166665
TCTTATA14150.015.035336537
TCGCCAT7000.014.813
CGCGCTT1001.09403525E-414.812
ACGCTAT1508.11051E-814.810
GTATAGA1803.3342076E-914.3888881
GTCCTAT3400.014.1470591
Produced by FastQC (version 0.11.5)