FastQCFastQC Report
Fri 10 Feb 2017
ERR1630775.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630775.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419535
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT94512.2527321915930734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81661.9464407022060137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60821.449700263386845No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38860.9262636013681814No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16550.39448436959967587No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT14290.34061520492926695No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC13340.3179710870368384No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12930.30819836247273763No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT12150.2896063498873753TruSeq Adapter, Index 1 (95% over 21bp)
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11870.28293229408750165No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA11540.2750664426090791No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.26648551372352725No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9710.23144672077419046No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.22334251016005816No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8930.2128547081888281No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.20498885671040556No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC8240.19640792782485372No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC8030.19140238597494846No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC7990.19044894943210935No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7850.18711192153217251No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG7430.17710083783236202No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC7390.17614740128952291No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG6880.16399108536832446No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT6850.16327600796119515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6850.16327600796119515No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6740.16065405746838762No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC6490.15469507907564325No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG6320.1506429737685771No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC6240.14873610068289891No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC6160.14682922759722072No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC6130.1461141501900914No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.1425387631544448No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT5940.1415853266116057No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5790.13800993957595908No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT5590.13324275686176362No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT5500.13109752464037566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5240.12490018711192154No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT5210.12418510970479221No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG5190.12370839143337267No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG5090.12132480007627491No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC5070.12084808180485536No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC5000.11917956785488695No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4980.1187028495834674No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.11488910341211102No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG4740.11298223032643283No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG4700.11202879378359373No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC4690.11179043464788396No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4680.11155207551217419No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC4590.10940684329078622No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT4570.10893012501936668No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA4540.10821504761223735No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTC4490.10702325193368849No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC4480.10678489279797872No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC4430.10559309711942984No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA4420.10535473798372007No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC4300.10249442835520278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTTA200.001840604137.01
TACCCTT250.005492415329.5999985
GAAGCAA250.005492415329.5999981
AACTTAT250.005492415329.5999982
GAATAAC250.005492415329.5999981
CATCGTT250.005492415329.59999828
TATAGTC250.005492415329.5999985
GCATCGT250.005492415329.59999827
AATAACG250.005492415329.5999982
AGATGCA250.005492415329.59999834
AACGCCG454.0002833E-628.7777795
ACAGAGG405.9312773E-527.753
GCAGCGT405.9312773E-527.751
CGTTTAT358.8599714E-426.4285731
TAAAGTT509.073132E-625.89999827
ATCTTAC509.073132E-625.8999981
CAGCGTC451.321256E-424.6666682
TAGCGCC451.321256E-424.66666829
AACAGAG451.321256E-424.6666682
TATTAGA751.3684257E-824.6666662
Produced by FastQC (version 0.11.5)