##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630775.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	419535
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51343749627564	31.0	31.0	34.0	30.0	34.0
2	31.631780423564184	31.0	31.0	34.0	30.0	34.0
3	31.644008247226097	31.0	31.0	34.0	28.0	34.0
4	35.38570798622284	37.0	35.0	37.0	33.0	37.0
5	35.357681719046084	37.0	35.0	37.0	33.0	37.0
6	35.473173871071545	37.0	35.0	37.0	33.0	37.0
7	35.40572061925703	37.0	35.0	37.0	33.0	37.0
8	35.46047171272957	37.0	35.0	37.0	33.0	37.0
9	36.98332439486575	39.0	37.0	39.0	33.0	39.0
10	36.844172715029735	39.0	37.0	39.0	33.0	39.0
11	37.01369134875517	39.0	37.0	39.0	33.0	39.0
12	36.95499302799528	39.0	37.0	39.0	33.0	39.0
13	37.024694006459534	39.0	37.0	39.0	33.0	39.0
14	38.01824877542994	40.0	37.0	41.0	33.0	41.0
15	37.999849833744506	40.0	37.0	41.0	33.0	41.0
16	37.873636287794824	40.0	37.0	41.0	33.0	41.0
17	37.98450188899615	40.0	37.0	41.0	33.0	41.0
18	38.02470592441632	40.0	37.0	41.0	33.0	41.0
19	38.13610306649028	40.0	37.0	41.0	33.0	41.0
20	38.119024634416675	40.0	38.0	41.0	33.0	41.0
21	38.062783796345954	40.0	37.0	41.0	33.0	41.0
22	37.93059697045538	40.0	37.0	41.0	33.0	41.0
23	37.781212532923355	40.0	37.0	41.0	33.0	41.0
24	37.6768207658479	39.0	37.0	41.0	33.0	41.0
25	37.587536200793735	39.0	36.0	41.0	32.0	41.0
26	37.32617779207933	39.0	36.0	41.0	32.0	41.0
27	37.07735469031189	39.0	35.0	40.0	31.0	41.0
28	36.84832254758244	39.0	35.0	40.0	31.0	41.0
29	36.695751248405976	39.0	35.0	40.0	31.0	41.0
30	36.43138951458162	38.0	35.0	40.0	30.0	41.0
31	36.250529753179116	38.0	35.0	40.0	30.0	41.0
32	35.96991192629935	38.0	35.0	40.0	30.0	41.0
33	35.70482319711109	38.0	35.0	40.0	29.0	41.0
34	35.51679359290643	38.0	35.0	40.0	28.0	41.0
35	35.2581143408774	38.0	34.0	40.0	27.0	41.0
36	35.09060269107464	38.0	34.0	40.0	26.0	41.0
37	34.73019414351604	37.0	34.0	40.0	24.0	41.0
38	34.37941053785739	37.0	33.0	40.0	23.0	41.0
39	34.09359886541051	37.0	33.0	40.0	21.0	41.0
40	33.637620222389074	37.0	33.0	40.0	18.0	41.0
41	33.344335991037696	36.0	33.0	40.0	17.0	41.0
42	32.89328661494273	36.0	32.0	40.0	15.0	41.0
43	32.00156601952161	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	1.0
11	7.0
12	1.0
13	3.0
14	1.0
15	1.0
16	8.0
17	10.0
18	45.0
19	79.0
20	187.0
21	346.0
22	643.0
23	1055.0
24	1716.0
25	2476.0
26	3614.0
27	5352.0
28	7047.0
29	9370.0
30	11587.0
31	13856.0
32	16901.0
33	21118.0
34	26421.0
35	33287.0
36	47304.0
37	73130.0
38	91863.0
39	52101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.34488183345847	19.924201794844294	14.044120276019878	19.686796095677355
2	19.964007770507823	22.811207646561073	32.16513520921973	25.059649373711373
3	23.109394925334	26.47645607637027	29.607780042189567	20.806368956106166
4	13.207002991407151	16.96068266056467	31.487718545532555	38.34459580249562
5	11.872430190568128	42.482033680145875	31.213605539466315	14.43193058981968
6	31.48199792627552	32.78415388465801	17.567783379217467	18.166064809849
7	26.104615824663018	33.86916467040891	20.88455075261897	19.141668752309105
8	32.9471915334835	28.432907862276092	18.847533578843244	19.772367025397166
9	28.144970026338683	11.746338207777658	22.14213355262374	37.96655821325992
10	21.27927348135436	28.989238085022706	28.059160737483168	21.672327696139774
11	32.47142669860679	24.10907314050079	19.111635501209673	24.307864659682743
12	22.230564791972064	30.756671076310678	25.90344071412397	21.109323417593288
13	30.848916061830362	19.28349243805642	28.98995316242983	20.877638337683386
14	22.364284267105248	22.732072413505428	28.966117248858858	25.93752607053047
15	27.495679740665263	26.445231029592286	26.54772545794749	19.51136377179496
16	23.164932603954377	24.803890021094784	29.084581739306614	22.946595635644226
17	22.36118559834102	28.037946774404997	27.655380361590808	21.945487265663175
18	20.595897839274436	22.074677917217873	32.792258095272146	24.537166148235546
19	20.430953317363272	26.287198922616707	33.26730785274172	20.014539907278298
20	22.091363056717555	22.649600152549848	34.53704696866769	20.721989822064906
21	23.77489363223569	24.41464955248072	32.68022930148855	19.130227513795035
22	24.74525367371018	24.484250420107976	31.136138820360635	19.634357085821208
23	21.766956273016554	23.947942364760983	32.58488564720464	21.70021571501782
24	19.515177517966322	27.045896051580915	32.344381279273485	21.094545151179283
25	20.10440130144088	25.916788825723717	33.112136055394664	20.866673817440738
26	20.9608256760461	27.38079063725315	31.94655988177387	19.711823804926883
27	21.44278784845126	26.645452703588496	32.486443324156504	19.425316123803736
28	19.669157519634833	26.680491496537833	33.17863825425769	20.471712729569642
29	19.298509063606133	26.95841824877543	34.02910365047017	19.71396903714827
30	16.85842659134518	28.986139416258478	34.32991287973589	19.825521112660446
31	20.48577591857652	28.60285792603716	30.867508074415724	20.043858080970598
32	19.093520206895732	27.667536677512004	32.2278236619114	21.01111945368086
33	17.845471772319353	27.90518073581465	32.27144338374629	21.977904108119702
34	19.769983434040068	28.282264888507513	31.954902451523708	19.99284922592871
35	18.190377441691396	28.778051890783846	33.23679788337087	19.794772784153885
36	19.32663544161989	29.180163752726234	30.885623368729664	20.607577436924213
37	19.570715196586697	27.6429856865339	32.21137688154742	20.574922235331975
38	18.508586887863945	26.81015886636395	31.842635298604407	22.838618947167696
39	19.844351484381516	28.035324823912187	31.53491365440309	20.585410037303202
40	18.088121372471903	27.78147234438128	31.854076537118477	22.276329746028342
41	18.504296423421167	28.195025444837736	31.39952566531994	21.901152466421156
42	18.550299736613155	28.43386129881893	32.08194787085702	20.933891093710894
43	17.118238049268832	26.830181033763573	31.34446470497098	24.707116211996617
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	119.0
2	157.0
3	433.0
4	709.0
5	709.0
6	1195.0
7	1681.0
8	1943.5
9	2206.0
10	3108.0
11	4010.0
12	4010.0
13	7150.5
14	10291.0
15	15500.0
16	20709.0
17	18259.5
18	15810.0
19	15810.0
20	16584.5
21	17359.0
22	12704.0
23	8049.0
24	7222.0
25	6395.0
26	6395.0
27	6659.0
28	6923.0
29	7445.0
30	7967.0
31	8850.0
32	9733.0
33	9733.0
34	10588.0
35	11443.0
36	12024.0
37	12605.0
38	14427.5
39	16250.0
40	16250.0
41	17795.5
42	19341.0
43	20812.0
44	22283.0
45	23530.0
46	24777.0
47	24777.0
48	27478.5
49	30180.0
50	29392.0
51	28604.0
52	27790.5
53	26977.0
54	26977.0
55	24522.5
56	22068.0
57	19325.0
58	16582.0
59	15408.0
60	14234.0
61	14234.0
62	14410.5
63	14587.0
64	14219.0
65	13851.0
66	13685.0
67	13519.0
68	13519.0
69	10917.5
70	8316.0
71	7145.5
72	5975.0
73	4222.0
74	2469.0
75	2469.0
76	1892.5
77	1316.0
78	1000.5
79	685.0
80	562.0
81	439.0
82	439.0
83	412.0
84	385.0
85	336.0
86	287.0
87	217.5
88	148.0
89	148.0
90	129.5
91	111.0
92	65.0
93	19.0
94	10.5
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	419535.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.0866193700214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.78987865881774	24.639276079956147
2	9.997874493200305	6.415959867451276
3	3.6819206946851994	3.544211636429063
4	2.0423631229807215	2.6213011256980248
5	1.2891081423153938	2.0681561144634713
6	0.8962933933272179	1.7255414973333196
7	0.6944792719227193	1.5598444440987722
8	0.5818332593676103	1.493524986411796
9	0.4074782479941703	1.1767139500456916
>10	3.031782751305068	18.936057209820724
>50	0.32626995973191575	7.349980461211373
>100	0.2301767524136118	14.690161267162441
>500	0.021602348932021818	4.703834524946825
>1k	0.0067041772547653915	3.410997078532324
>5k	0.0022347257515884637	5.66443975643876
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9451	2.2527321915930734	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8166	1.9464407022060137	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6082	1.449700263386845	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3886	0.9262636013681814	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1655	0.39448436959967587	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	1429	0.34061520492926695	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1334	0.3179710870368384	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1293	0.30819836247273763	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	1215	0.2896063498873753	TruSeq Adapter, Index 1 (95% over 21bp)
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1187	0.28293229408750165	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1154	0.2750664426090791	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1118	0.26648551372352725	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	971	0.23144672077419046	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	937	0.22334251016005816	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	893	0.2128547081888281	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	860	0.20498885671040556	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	824	0.19640792782485372	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	803	0.19140238597494846	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	799	0.19044894943210935	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	785	0.18711192153217251	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	743	0.17710083783236202	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	739	0.17614740128952291	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	688	0.16399108536832446	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	685	0.16327600796119515	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	685	0.16327600796119515	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	674	0.16065405746838762	No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	649	0.15469507907564325	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	632	0.1506429737685771	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	624	0.14873610068289891	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	616	0.14682922759722072	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	613	0.1461141501900914	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	598	0.1425387631544448	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	594	0.1415853266116057	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	579	0.13800993957595908	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	559	0.13324275686176362	No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT	550	0.13109752464037566	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	524	0.12490018711192154	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	521	0.12418510970479221	No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG	519	0.12370839143337267	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	509	0.12132480007627491	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC	507	0.12084808180485536	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	500	0.11917956785488695	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	498	0.1187028495834674	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	482	0.11488910341211102	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	474	0.11298223032643283	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	470	0.11202879378359373	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	469	0.11179043464788396	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	468	0.11155207551217419	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	459	0.10940684329078622	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT	457	0.10893012501936668	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	454	0.10821504761223735	No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTC	449	0.10702325193368849	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	448	0.10678489279797872	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	443	0.10559309711942984	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	442	0.10535473798372007	No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC	430	0.10249442835520278	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.383591357097739E-4	0.0	0.0	4.767182714195478E-4	0.0
3	2.383591357097739E-4	0.0	0.0	4.767182714195478E-4	0.0
4	2.383591357097739E-4	0.0	0.0	4.767182714195478E-4	0.0
5	2.383591357097739E-4	0.0	0.0	4.767182714195478E-4	0.0
6	2.383591357097739E-4	0.0	0.0	4.767182714195478E-4	0.0
7	4.767182714195478E-4	0.0	0.0	9.534365428390956E-4	0.0
8	4.767182714195478E-4	0.0	0.0	9.534365428390956E-4	0.0
9	4.767182714195478E-4	0.0	0.0	0.0035753870356466087	0.0
10	9.534365428390956E-4	2.383591357097739E-4	0.0	0.006197337528454121	0.0
11	9.534365428390956E-4	2.383591357097739E-4	0.0	0.007150774071293217	0.0
12	0.0011917956785488695	7.150774071293217E-4	0.0	0.009534365428390956	0.0
13	0.0011917956785488695	7.150774071293217E-4	0.0	0.010249442835520279	0.0
14	0.0019068730856781912	7.150774071293217E-4	0.0	0.01334811159974734	0.0
15	0.0019068730856781912	7.150774071293217E-4	0.0	0.018115294313942817	0.0
16	0.002145232221387965	7.150774071293217E-4	0.0	0.027411300606624	0.0
17	0.002145232221387965	9.534365428390956E-4	0.0	0.04147448961350066	0.0
18	0.002145232221387965	9.534365428390956E-4	0.0	0.04552659492056682	0.0
19	0.002383591357097739	9.534365428390956E-4	0.0	0.058159629113184834	0.0
20	0.002383591357097739	9.534365428390956E-4	0.0	0.06316517096309009	0.0
21	0.002383591357097739	9.534365428390956E-4	0.0	0.08437913404125996	0.0
22	0.002383591357097739	0.0011917956785488695	0.0	0.11798777217633809	0.0
23	0.002383591357097739	0.0011917956785488695	0.0	0.1744788873395545	0.0
24	0.002383591357097739	0.0011917956785488695	0.0	0.261479971873622	0.0
25	0.002383591357097739	0.0011917956785488695	0.0	0.2769733156947573	0.0
26	0.003337027899936835	0.0011917956785488695	0.0	0.3191628827153873	0.0
27	0.003337027899936835	0.0011917956785488695	0.0	0.35157972517191655	0.0
28	0.003337027899936835	0.0011917956785488695	0.0	0.41259966391361863	0.0
29	0.003337027899936835	0.0011917956785488695	0.0	0.5067515225189794	0.0
30	0.003337027899936835	0.0011917956785488695	0.0	0.6879044656584076	0.0
31	0.003337027899936835	0.0011917956785488695	0.0	1.1822613131204787	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACTTA	20	0.0018406041	37.0	1
TACCCTT	25	0.0054924153	29.599998	5
GAAGCAA	25	0.0054924153	29.599998	1
AACTTAT	25	0.0054924153	29.599998	2
GAATAAC	25	0.0054924153	29.599998	1
CATCGTT	25	0.0054924153	29.599998	28
TATAGTC	25	0.0054924153	29.599998	5
GCATCGT	25	0.0054924153	29.599998	27
AATAACG	25	0.0054924153	29.599998	2
AGATGCA	25	0.0054924153	29.599998	34
AACGCCG	45	4.0002833E-6	28.777779	5
ACAGAGG	40	5.9312773E-5	27.75	3
GCAGCGT	40	5.9312773E-5	27.75	1
CGTTTAT	35	8.8599714E-4	26.42857	31
TAAAGTT	50	9.073132E-6	25.899998	27
ATCTTAC	50	9.073132E-6	25.899998	1
CAGCGTC	45	1.321256E-4	24.666668	2
TAGCGCC	45	1.321256E-4	24.666668	29
AACAGAG	45	1.321256E-4	24.666668	2
TATTAGA	75	1.3684257E-8	24.666666	2
>>END_MODULE