FastQCFastQC Report
Fri 10 Feb 2017
ERR1630774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924311
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101970.5299039500371822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88390.4593332366753607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78690.4089255842740597No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC73960.38434535789693036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61160.31782804338799703No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT44800.23281060078126667No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG29220.15184655702742436No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT26610.13828326086583717No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG25970.1349573951403905No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT25160.13074809633162207No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG25050.13017646316006093No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT23370.12144606563076342No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG22470.11676906695435404No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG21320.11079290197894207No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC21220.11027323545934103No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG21150.1099094688956203No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT21050.10938980237601927No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT20260.10528443687117103No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG19640.10206250444964457No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT19350.10055547154280155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGC19350.017.4961227
TGCATCA19300.017.34974114
CAGGACA24800.016.9334684
CCAGGAC22450.016.8930973
ATCAAGC20050.016.88528830
TCCAGGA22600.016.6172562
CGAATTA904.448464E-516.44444515
GCATCAG20750.016.31566215
TCACGTA4650.016.31182725
GGACAGG22550.016.2439026
ACAGGCT21150.016.1820328
TACTCCG5600.015.8571425
GGCCATC21550.015.6241326
AGGCCAT21800.015.61467825
ATCACGT4900.015.47959224
CATCAAG22000.015.47272829
CCGGTCT5150.015.4466019
GCCTAAT1201.9370545E-615.4166661
ATCAGAA21850.015.40961217
GCTGCAT22000.015.30454612
Produced by FastQC (version 0.11.5)