FastQCFastQC Report
Fri 10 Feb 2017
ERR1630773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173885
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.36323830698918547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40250.34287856135822503No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29920.25488016287796506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.16756326215941084No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG16650.14183672165501732No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT14630.12462890317194615No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG14020.11943248273893951No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA13790.11747317667403535No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA13330.11355456454422708No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC13130.1118508201399626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCT450.00382585520.5555574
CTTATAC6350.019.22834637
TATAGAG801.6167176E-518.52
GGTATCA20350.017.2727281
CGAATTA752.0673129E-417.26666615
AACCGCG650.001579961317.0769237
CTAAACG650.001579961317.07692326
CGCCGTA650.001579961317.07692325
TAGGTAC803.382641E-416.187522
CGCAATA803.382641E-416.187536
ATAACGC1151.2421733E-616.0869563
CTATACT1751.3096724E-1015.8571434
GTGCGCA700.00259253715.8571439
CTAAGGT957.059882E-515.5789484
TATACCC855.36427E-415.2352945
TCTTATA10350.015.01449337
TCGTTTA750.00410447414.830
GTAAACG750.00410447414.827
TCTAGCG908.274197E-414.38888928
TAGGTTA1458.9096466E-714.0344846
Produced by FastQC (version 0.11.5)