##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630773.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1173885
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.840256924656163	33.0	31.0	34.0	30.0	34.0
2	32.03460986382823	33.0	31.0	34.0	30.0	34.0
3	32.12859010891186	34.0	31.0	34.0	30.0	34.0
4	35.77000217227412	37.0	35.0	37.0	35.0	37.0
5	35.708149435421696	37.0	35.0	37.0	33.0	37.0
6	35.77940598951346	37.0	35.0	37.0	35.0	37.0
7	35.73982459951358	37.0	35.0	37.0	33.0	37.0
8	35.733584635632965	37.0	35.0	37.0	33.0	37.0
9	37.41197732316198	39.0	37.0	39.0	34.0	39.0
10	37.302394186824095	39.0	37.0	39.0	34.0	39.0
11	37.39605668357633	39.0	37.0	39.0	34.0	39.0
12	37.3437466191322	39.0	37.0	39.0	34.0	39.0
13	37.37797995544708	39.0	37.0	39.0	34.0	39.0
14	38.63089655289913	40.0	38.0	41.0	34.0	41.0
15	38.639279827240316	40.0	38.0	41.0	34.0	41.0
16	38.59709596766293	40.0	38.0	41.0	34.0	41.0
17	38.5833808252086	40.0	38.0	41.0	34.0	41.0
18	38.561193813704065	40.0	38.0	41.0	34.0	41.0
19	38.64022199789588	40.0	38.0	41.0	34.0	41.0
20	38.59778342853005	40.0	38.0	41.0	34.0	41.0
21	38.56022097564923	40.0	38.0	41.0	34.0	41.0
22	38.50113682345375	40.0	38.0	41.0	34.0	41.0
23	38.44221367510446	40.0	38.0	41.0	34.0	41.0
24	38.38960204790077	40.0	38.0	41.0	34.0	41.0
25	38.34103510991281	40.0	38.0	41.0	34.0	41.0
26	38.19813780736614	40.0	38.0	41.0	34.0	41.0
27	38.070012820676645	40.0	38.0	41.0	33.0	41.0
28	37.98640497152617	40.0	37.0	41.0	33.0	41.0
29	37.911366956729154	40.0	37.0	41.0	33.0	41.0
30	37.81708344514156	40.0	37.0	41.0	33.0	41.0
31	37.780449532961065	40.0	37.0	41.0	33.0	41.0
32	37.682757680692745	40.0	37.0	41.0	33.0	41.0
33	37.617657607005796	40.0	37.0	41.0	33.0	41.0
34	37.55339151620474	40.0	37.0	41.0	32.0	41.0
35	37.50767749822172	40.0	37.0	41.0	32.0	41.0
36	37.48420075220316	40.0	37.0	41.0	32.0	41.0
37	37.362267172678756	40.0	36.0	41.0	32.0	41.0
38	37.21630142646	40.0	36.0	41.0	31.0	41.0
39	37.1610089574362	39.0	36.0	41.0	31.0	41.0
40	36.99822384645856	39.0	36.0	41.0	31.0	41.0
41	36.94783390195803	39.0	36.0	41.0	31.0	41.0
42	36.82798996494546	39.0	36.0	41.0	30.0	41.0
43	36.03281326535393	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	8.0
12	0.0
13	1.0
14	3.0
15	3.0
16	11.0
17	18.0
18	56.0
19	87.0
20	181.0
21	324.0
22	609.0
23	1041.0
24	1840.0
25	2870.0
26	4285.0
27	6437.0
28	9362.0
29	13219.0
30	18018.0
31	23416.0
32	30026.0
33	38778.0
34	51581.0
35	69302.0
36	97990.0
37	155536.0
38	298912.0
39	349964.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.83494550147587	19.786435638925447	12.756189916388744	24.62242894320994
2	18.196586548086056	21.608079155965022	35.824037277927566	24.371297018021355
3	20.005963105414924	23.90421548959225	30.56508942528442	25.524731979708402
4	14.661487283677701	15.923876700017464	34.767545372843166	34.64709064346167
5	13.757736064435614	37.964877309106086	34.58839664873476	13.688989977723542
6	34.08332162009056	35.28820966278639	16.0503797220341	14.578088995088956
7	28.107097373252067	31.405887288788936	21.79029462000111	18.696720717957895
8	26.431975874979237	34.7284444387653	20.34747867124974	18.49210101500573
9	26.13135017484677	14.307789945352397	20.333678341575197	39.227181538225636
10	16.32101952065151	27.8406317484251	33.79743330905497	22.040915421868412
11	34.46470480498516	22.304740242868764	22.367949160266974	20.8626057918791
12	20.740532505313553	26.522529890065893	29.7268471783863	23.010090426234257
13	29.263173138765726	20.600825464163865	26.174199346614017	23.96180205045639
14	22.37033440243295	21.728533885346522	25.962338729943735	29.938792982276794
15	25.11455551438173	28.507051372153146	23.397181154883146	22.981211958581973
16	24.44881738841539	27.379854074291778	24.66391511945378	23.507413417839057
17	23.038883706666326	27.46708578779011	26.158439710874575	23.335590794668985
18	22.05752692980999	26.02682545564514	28.109652989858464	23.805994624686406
19	23.45519365184835	26.400371416280127	28.069103873036966	22.075331058834553
20	23.864603432193103	25.525072728589258	28.059562904373088	22.550760934844554
21	23.583059669388398	26.60260587706632	26.717438249913748	23.09689620363153
22	23.757608283605293	26.07018575073368	26.84206715308569	23.33013881257534
23	22.958466970785043	26.4761880422699	27.230009753936713	23.335335233008344
24	24.05508205658987	26.468606379670923	26.85143774730915	22.624873816430057
25	23.694484553427294	25.876384824748595	27.22983937949629	23.199291242327828
26	23.563125859858506	26.41280875043126	27.00204875264613	23.02201663706411
27	23.651379819999406	26.095401167916787	26.603968872589732	23.649250139494075
28	22.823445226747083	26.40352334342802	27.528165024683	23.244866405141902
29	22.93461454912534	26.914987413588214	27.327463933860642	22.822934103425805
30	22.536449481848734	27.58268484561946	27.166204525997014	22.714661146534798
31	23.29427499286557	26.675440950348627	27.118840431558453	22.911443625227342
32	22.406453783803354	26.7557724990097	27.24193596476657	23.595837752420383
33	22.682204815633558	26.36817064703953	27.53770599334688	23.411918543980033
34	23.07943282348782	26.166276935134192	27.358983205339538	23.395307036038453
35	23.156953193881854	26.30896552899134	27.49494200879984	23.039139268326966
36	22.832645446530112	26.593746406164147	27.305826379926483	23.267781767379258
37	23.54293648866797	25.530609897903116	27.141074296034105	23.785379317394803
38	22.657585708991938	25.970857451965056	28.16263944083109	23.20891739821192
39	22.698816323575137	25.761808013561804	28.212814713536677	23.32656094932638
40	22.369567717451027	25.29574873177526	28.924468751197946	23.41021479957577
41	21.760734654587118	25.939423367706375	29.020304373937822	23.27953760376868
42	22.351508026765824	25.38613237242149	28.674955383193414	23.587404217619273
43	21.646243030620546	25.36534669068946	28.788850696618496	24.199559582071497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	153.0
1	145.0
2	137.0
3	403.5
4	670.0
5	670.0
6	906.0
7	1142.0
8	1211.0
9	1280.0
10	1900.5
11	2521.0
12	2521.0
13	4084.5
14	5648.0
15	8531.0
16	11414.0
17	11294.5
18	11175.0
19	11175.0
20	12863.5
21	14552.0
22	15044.0
23	15536.0
24	17488.0
25	19440.0
26	19440.0
27	21775.0
28	24110.0
29	30604.5
30	37099.0
31	39380.0
32	41661.0
33	41661.0
34	45054.5
35	48448.0
36	51177.5
37	53907.0
38	60734.5
39	67562.0
40	67562.0
41	72033.5
42	76505.0
43	72980.5
44	69456.0
45	72711.0
46	75966.0
47	75966.0
48	78019.0
49	80072.0
50	82291.0
51	84510.0
52	87752.0
53	90994.0
54	90994.0
55	81378.5
56	71763.0
57	67273.0
58	62783.0
59	56865.0
60	50947.0
61	50947.0
62	47616.0
63	44285.0
64	39206.0
65	34127.0
66	29656.5
67	25186.0
68	25186.0
69	21664.0
70	18142.0
71	15772.0
72	13402.0
73	10752.0
74	8102.0
75	8102.0
76	6423.5
77	4745.0
78	3847.0
79	2949.0
80	2268.5
81	1588.0
82	1588.0
83	1211.0
84	834.0
85	683.5
86	533.0
87	434.5
88	336.0
89	336.0
90	255.0
91	174.0
92	99.0
93	24.0
94	15.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1173885.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.43622289067979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.11682910163366	51.49214665803004
2	7.181815340494564	8.393563239936874
3	1.753744478298067	3.0744660968137434
4	0.785675480159536	1.8364762991337806
5	0.42808632211177755	1.250787386768759
6	0.2705531851543647	0.948606373887829
7	0.19268225249612184	0.7881736137759132
8	0.14781380264210622	0.6910144254010457
9	0.11646821215056245	0.6125366164418323
>10	0.7710019890273521	8.978552111947822
>50	0.11878123590961492	4.940997329777576
>100	0.10851580342380342	12.410335678009503
>500	0.00642623719890266	2.621847477318658
>1k	0.001606559299725665	1.960496692756735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4264	0.36323830698918547	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4025	0.34287856135822503	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2992	0.25488016287796506	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1967	0.16756326215941084	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1665	0.14183672165501732	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1463	0.12462890317194615	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1402	0.11943248273893951	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1379	0.11747317667403535	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1333	0.11355456454422708	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1313	0.1118508201399626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.7037444042644723E-4	0.0
3	0.0	0.0	0.0	2.5556166063967086E-4	0.0
4	0.0	0.0	0.0	3.4074888085289446E-4	0.0
5	8.518722021322362E-5	0.0	0.0	3.4074888085289446E-4	0.0
6	8.518722021322362E-5	0.0	0.0	3.4074888085289446E-4	0.0
7	8.518722021322362E-5	0.0	0.0	5.111233212793417E-4	0.0
8	8.518722021322362E-5	0.0	0.0	5.111233212793417E-4	0.0
9	8.518722021322362E-5	0.0	0.0	0.0016185571840512487	0.0
10	8.518722021322362E-5	0.0	0.0	0.002385242165970261	0.0
11	8.518722021322362E-5	0.0	0.0	0.0029815527074628263	0.0
12	8.518722021322362E-5	0.0	0.0	0.003322301588315721	0.0
13	8.518722021322362E-5	0.0	0.0	0.0034074888085289445	0.0
14	8.518722021322362E-5	0.0	0.0	0.0035778632489553916	0.0
15	8.518722021322362E-5	0.0	0.0	0.0038334249095950627	0.0
16	8.518722021322362E-5	0.0	0.0	0.005026045992580193	0.0
17	8.518722021322362E-5	0.0	0.0	0.00732610093833723	0.0
18	8.518722021322362E-5	0.0	0.0	0.00826316036068269	0.0
19	8.518722021322362E-5	0.0	0.0	0.010478028086226504	0.0
20	8.518722021322362E-5	0.0	0.0	0.011670649169211634	0.0
21	8.518722021322362E-5	0.0	0.0	0.014396640216034791	0.0
22	1.7037444042644723E-4	0.0	0.0	0.018741188446909196	0.0
23	1.7037444042644723E-4	0.0	0.0	0.025982102165033202	0.0
24	1.7037444042644723E-4	0.0	0.0	0.037567564114031615	0.0
25	1.7037444042644723E-4	0.0	0.0	0.041656550684266345	0.0
26	1.7037444042644723E-4	0.0	0.0	0.04651222223642009	0.0
27	1.7037444042644723E-4	0.0	0.0	0.055797629239661466	0.0
28	1.7037444042644723E-4	0.0	0.0	0.07837224259616572	0.0
29	1.7037444042644723E-4	0.0	0.0	0.11585461948998411	0.0
30	1.7037444042644723E-4	0.0	0.0	0.17301524425305714	0.0
31	1.7037444042644723E-4	0.0	0.0	0.41145427362987	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCT	45	0.003825855	20.555557	4
CTTATAC	635	0.0	19.228346	37
TATAGAG	80	1.6167176E-5	18.5	2
GGTATCA	2035	0.0	17.272728	1
CGAATTA	75	2.0673129E-4	17.266666	15
AACCGCG	65	0.0015799613	17.076923	7
CTAAACG	65	0.0015799613	17.076923	26
CGCCGTA	65	0.0015799613	17.076923	25
TAGGTAC	80	3.382641E-4	16.1875	22
CGCAATA	80	3.382641E-4	16.1875	36
ATAACGC	115	1.2421733E-6	16.086956	3
CTATACT	175	1.3096724E-10	15.857143	4
GTGCGCA	70	0.002592537	15.857143	9
CTAAGGT	95	7.059882E-5	15.578948	4
TATACCC	85	5.36427E-4	15.235294	5
TCTTATA	1035	0.0	15.014493	37
TCGTTTA	75	0.004104474	14.8	30
GTAAACG	75	0.004104474	14.8	27
TCTAGCG	90	8.274197E-4	14.388889	28
TAGGTTA	145	8.9096466E-7	14.034484	6
>>END_MODULE