FastQCFastQC Report
Fri 10 Feb 2017
ERR1630772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943633
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31740.336359580472493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27790.29450008636832326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22510.2385461296923698No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16290.1726306731536519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG358.868214E-426.428575
TATACAG751.3731551E-824.6666665
GTGCTAA656.900457E-519.9230771
AAGACGG1800.019.5277775
CCGTGAG500.007033971618.59
GGTATCA14550.018.3092781
TTAGAGT1201.0405893E-716.9583324
GTGTAGT1201.0405893E-716.9583321
ACGGACC2000.016.6500028
GCGTTAT1851.8189894E-1116.01
GTATTGA700.002592032515.8571421
CCTATGC700.002592032515.8571423
TAGGTTA1659.731593E-1015.696976
CGGACCA2150.015.4883749
CGTTATT1802.0190782E-1015.4166662
CTTATAC4200.015.41666637
TCTTTCG1455.3410986E-815.31034510
ACGTTAA750.00410368214.820
CTATCCT1508.094503E-814.84
TTTCGAG1508.094503E-814.812
Produced by FastQC (version 0.11.5)