##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630772.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	943633
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09559330799156	31.0	31.0	34.0	28.0	34.0
2	31.282496479033693	31.0	31.0	34.0	28.0	34.0
3	31.367726647965892	31.0	31.0	34.0	28.0	34.0
4	35.14980188272347	37.0	35.0	37.0	32.0	37.0
5	34.809549899166306	35.0	35.0	37.0	32.0	37.0
6	34.872918814835856	35.0	35.0	37.0	32.0	37.0
7	34.85674621383525	35.0	35.0	37.0	32.0	37.0
8	34.83534276567267	35.0	35.0	37.0	32.0	37.0
9	36.41333760052902	38.0	35.0	39.0	32.0	39.0
10	36.13455654899733	37.0	35.0	39.0	32.0	39.0
11	36.35463045484844	38.0	35.0	39.0	32.0	39.0
12	36.23166315718081	37.0	35.0	39.0	32.0	39.0
13	36.3354195963897	38.0	35.0	39.0	32.0	39.0
14	37.34638996304707	39.0	36.0	40.0	32.0	41.0
15	37.29541781603653	39.0	36.0	40.0	32.0	41.0
16	37.25843733739706	39.0	36.0	40.0	32.0	41.0
17	37.207279736931625	39.0	36.0	40.0	32.0	41.0
18	37.16732670434374	38.0	36.0	40.0	32.0	41.0
19	37.18489815426124	39.0	36.0	40.0	32.0	41.0
20	37.146228459581216	39.0	36.0	40.0	32.0	41.0
21	37.069427415107356	39.0	36.0	40.0	31.0	41.0
22	37.007592994310286	39.0	36.0	40.0	31.0	41.0
23	36.962880696202866	39.0	36.0	40.0	31.0	41.0
24	36.88958313242542	39.0	36.0	40.0	31.0	41.0
25	36.80907619805581	38.0	36.0	40.0	31.0	41.0
26	36.54674009916991	38.0	35.0	40.0	30.0	41.0
27	36.40325211178499	38.0	35.0	40.0	30.0	41.0
28	36.27376533037738	38.0	35.0	40.0	30.0	41.0
29	36.13109863686412	38.0	35.0	40.0	30.0	41.0
30	36.032805126569336	38.0	35.0	40.0	29.0	41.0
31	35.96753398831961	38.0	35.0	40.0	29.0	41.0
32	35.856536386497716	38.0	34.0	40.0	29.0	41.0
33	35.80445893689602	38.0	34.0	40.0	29.0	41.0
34	35.733987683771126	38.0	34.0	40.0	29.0	41.0
35	35.651504345439385	38.0	34.0	40.0	28.0	41.0
36	35.6046821168823	38.0	34.0	40.0	28.0	41.0
37	35.382287393509976	38.0	34.0	40.0	27.0	41.0
38	35.22859416743586	38.0	34.0	40.0	27.0	41.0
39	35.1506634464882	38.0	34.0	40.0	26.0	41.0
40	35.033828829640335	38.0	33.0	40.0	26.0	41.0
41	34.865439212066555	38.0	33.0	40.0	26.0	41.0
42	34.772957283181064	38.0	33.0	40.0	25.0	41.0
43	33.80848168726613	37.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	1.0
13	5.0
14	5.0
15	16.0
16	34.0
17	77.0
18	109.0
19	238.0
20	428.0
21	815.0
22	1369.0
23	2144.0
24	3325.0
25	5247.0
26	7670.0
27	10766.0
28	14719.0
29	19403.0
30	25167.0
31	32465.0
32	41789.0
33	53241.0
34	70258.0
35	94376.0
36	132879.0
37	179851.0
38	183625.0
39	63605.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.5029646059432	20.168963993416934	12.634572974874766	24.6934984257651
2	19.412737791069198	22.079664445817386	33.97390722876372	24.533690534349688
3	21.222233643800077	24.068785216286418	29.339372404313963	25.36960873559954
4	15.811655590679852	16.121203900245114	33.590071563838904	34.47706894523612
5	14.329405605781062	37.45884257968935	34.09408106753367	14.11767074699592
6	34.94981629510626	34.54256050816366	15.528388685007839	14.979234511722247
7	28.471556208822708	30.837306452826468	21.729528322981498	18.961609015369323
8	26.939286777804504	33.64348215884777	20.25480244968118	19.162428613666542
9	26.651674962617882	13.975772360652924	20.06246072360759	39.310091953121606
10	17.01943446233864	27.149538008950515	32.97892294991803	22.852104578792815
11	35.22057833924841	21.89505877814786	21.714056206173375	21.17030667643035
12	21.33106832847092	26.00947614167796	28.9085905219508	23.75086500790032
13	29.44078895078913	20.207856232242833	26.01678830647084	24.334566510497197
14	22.944937279641557	21.52521160239203	25.377556740809194	30.152294377157222
15	25.65065019981285	27.994993816451945	22.790216111560323	23.564139872174884
16	25.22283557272796	26.733698376381497	24.307331345978785	23.73613470491176
17	23.587348047387067	26.917880150439842	25.733521400798825	23.761250401374262
18	23.14247170245212	25.270311657180283	27.46586861629468	24.12134802407292
19	24.06666574823051	25.79710544247605	27.276706092304952	22.85952271698849
20	24.47943215211846	24.822680003772653	27.26971184772046	23.428175996388426
21	24.407688158425998	25.83059303775938	26.290517605891274	23.471201197923346
22	24.36508685050226	25.409030841439417	26.505219719954688	23.72066258810364
23	23.531394090711114	25.74496652830073	26.893082374185727	23.830557006802433
24	24.21004776221264	25.949601169098578	26.539343155654795	23.301007913033985
25	24.244489118121134	25.32605366705064	26.776193710902437	23.653263503925785
26	23.92190608001204	26.304929988671443	26.562233410658592	23.210930520657925
27	24.374412509948254	25.5443588768091	26.062356869672847	24.0188717435698
28	23.503629059178728	25.745496395314703	27.089345116162743	23.661529429343823
29	23.270911466640104	26.209447952752818	27.015269707608784	23.504370872998294
30	23.18613274440381	26.699468967278595	26.95889185732165	23.15550643099595
31	23.793042422212874	26.24113400018863	26.4956821137031	23.47014146389539
32	23.130496707936242	25.999620615217996	26.712079802211242	24.157802874634523
33	23.13187436217258	25.861749218181224	27.082350871578253	23.924025548067945
34	23.954969781684195	25.753338427121562	26.6695844676903	23.622107323503947
35	23.880576452921844	25.715611895726408	27.126753727349513	23.277057924002236
36	23.351133332556195	26.17712606490023	26.668736680467937	23.80300392207564
37	24.07461375344016	25.14229578660348	26.85673349702692	23.926356962929443
38	23.41069038492719	25.25770082224763	27.433970622053277	23.897638170771902
39	23.58755999419266	25.37713284719801	27.195742412569295	23.83956474604004
40	22.94938816255896	25.182141786054537	28.05614046986487	23.81232958152163
41	22.461698562894686	25.517865526110256	28.217537962322215	23.80289794867284
42	23.387588183117803	25.04999295276871	27.870475068167394	23.691943795946095
43	22.747190910025402	24.751465877094166	28.033992028680643	24.46735118419979
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	146.0
1	140.5
2	135.0
3	291.0
4	447.0
5	447.0
6	653.5
7	860.0
8	908.0
9	956.0
10	1411.5
11	1867.0
12	1867.0
13	3166.0
14	4465.0
15	6333.0
16	8201.0
17	8100.0
18	7999.0
19	7999.0
20	9755.0
21	11511.0
22	11602.5
23	11694.0
24	12809.5
25	13925.0
26	13925.0
27	15582.0
28	17239.0
29	20749.0
30	24259.0
31	26845.5
32	29432.0
33	29432.0
34	32285.0
35	35138.0
36	38269.5
37	41401.0
38	46433.0
39	51465.0
40	51465.0
41	53957.0
42	56449.0
43	55440.0
44	54431.0
45	57217.0
46	60003.0
47	60003.0
48	62003.0
49	64003.0
50	66005.5
51	68008.0
52	71453.5
53	74899.0
54	74899.0
55	68380.5
56	61862.0
57	58650.0
58	55438.0
59	50515.5
60	45593.0
61	45593.0
62	42460.5
63	39328.0
64	35201.0
65	31074.0
66	27277.5
67	23481.0
68	23481.0
69	20246.5
70	17012.0
71	14861.5
72	12711.0
73	10196.5
74	7682.0
75	7682.0
76	5924.5
77	4167.0
78	3426.5
79	2686.0
80	2079.5
81	1473.0
82	1473.0
83	1151.5
84	830.0
85	715.5
86	601.0
87	527.5
88	454.0
89	454.0
90	354.0
91	254.0
92	145.5
93	37.0
94	24.5
95	12.0
96	12.0
97	8.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	943633.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.06589244678112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.77245802232862	51.54651999760925
2	6.361589883127255	7.387827878883961
3	1.670183722846855	2.909421252515699
4	0.7477514525488758	1.7367542168250947
5	0.4332753300426672	1.2579259357050552
6	0.28159591625120334	0.9810670911897095
7	0.21173752833617845	0.8606309983121065
8	0.1610190195764773	0.74797704580911
9	0.12351020763160869	0.6454557389174936
>10	0.9621926287463176	11.541826736271746
>50	0.15276945029074165	6.30432160955587
>100	0.11916269284317255	12.310682231206243
>500	0.002019706648736051	0.7212478245932711
>1k	7.34438781358564E-4	1.0483414426054154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3174	0.336359580472493	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2779	0.29450008636832326	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2251	0.2385461296923698	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1629	0.1726306731536519	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0597340279536642E-4	0.0
2	0.0	0.0	0.0	8.477872223629314E-4	0.0
3	0.0	0.0	0.0	0.001271680833544397	0.0
4	0.0	0.0	0.0	0.0022254414587026946	0.0
5	0.0	0.0	0.0	0.002437388264293428	0.0
6	0.0	0.0	0.0	0.002543361667088794	0.0
7	0.0	0.0	0.0	0.0028612818754748933	0.0
8	0.0	0.0	0.0	0.00296725527827026	0.0
9	0.0	0.0	0.0	0.005298670139768321	0.0
10	0.0	0.0	0.0	0.0072061913900849164	1.0597340279536642E-4
11	0.0	0.0	0.0	0.007842031806857115	1.0597340279536642E-4
12	0.0	0.0	0.0	0.008689819029220046	1.0597340279536642E-4
13	0.0	0.0	0.0	0.008795792432015413	1.0597340279536642E-4
14	0.0	0.0	0.0	0.010597340279536642	1.0597340279536642E-4
15	0.0	0.0	0.0	0.011657074307490306	1.0597340279536642E-4
16	0.0	0.0	0.0	0.0148362763913513	1.0597340279536642E-4
17	0.0	0.0	0.0	0.017485611461235458	1.0597340279536642E-4
18	0.0	0.0	0.0	0.019075212503165954	1.0597340279536642E-4
19	0.0	0.0	0.0	0.020770786947891817	1.0597340279536642E-4
20	0.0	0.0	0.0	0.022678308198208413	1.0597340279536642E-4
21	0.0	0.0	0.0	0.026175430490455506	1.0597340279536642E-4
22	1.0597340279536642E-4	0.0	0.0	0.029990472991088698	1.0597340279536642E-4
23	1.0597340279536642E-4	0.0	0.0	0.038892238825899476	1.0597340279536642E-4
24	1.0597340279536642E-4	0.0	0.0	0.05309267480047858	1.0597340279536642E-4
25	1.0597340279536642E-4	0.0	0.0	0.056377850287134934	1.0597340279536642E-4
26	1.0597340279536642E-4	0.0	0.0	0.06231236084367545	1.0597340279536642E-4
27	1.0597340279536642E-4	0.0	0.0	0.07407540855396112	1.0597340279536642E-4
28	1.0597340279536642E-4	0.0	0.0	0.09675371675216954	1.0597340279536642E-4
29	1.0597340279536642E-4	0.0	0.0	0.12769795036841652	1.0597340279536642E-4
30	1.0597340279536642E-4	0.0	0.0	0.17909505072416926	1.0597340279536642E-4
31	1.0597340279536642E-4	0.0	0.0	0.35363324512813776	1.0597340279536642E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCCG	35	8.868214E-4	26.42857	5
TATACAG	75	1.3731551E-8	24.666666	5
GTGCTAA	65	6.900457E-5	19.923077	1
AAGACGG	180	0.0	19.527777	5
CCGTGAG	50	0.0070339716	18.5	9
GGTATCA	1455	0.0	18.309278	1
TTAGAGT	120	1.0405893E-7	16.958332	4
GTGTAGT	120	1.0405893E-7	16.958332	1
ACGGACC	200	0.0	16.650002	8
GCGTTAT	185	1.8189894E-11	16.0	1
GTATTGA	70	0.0025920325	15.857142	1
CCTATGC	70	0.0025920325	15.857142	3
TAGGTTA	165	9.731593E-10	15.69697	6
CGGACCA	215	0.0	15.488374	9
CGTTATT	180	2.0190782E-10	15.416666	2
CTTATAC	420	0.0	15.416666	37
TCTTTCG	145	5.3410986E-8	15.310345	10
ACGTTAA	75	0.004103682	14.8	20
CTATCCT	150	8.094503E-8	14.8	4
TTTCGAG	150	8.094503E-8	14.8	12
>>END_MODULE