FastQCFastQC Report
Fri 10 Feb 2017
ERR1630771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419428
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT96350.6787945566805784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95740.6744970509247387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69080.4866749141203358No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.3555657631102106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG551.9030671E-523.5454545
TATACGT450.00382624420.5555572
TAGGACA951.676799E-719.4736844
CGAGTAA500.00703567618.510
GGTTAGT500.00703567618.58
GGTATCA48700.016.4866521
TTCGGAA2400.016.187531
CAAGACG3250.015.9384614
TACCCCG1403.4746336E-815.8571435
TTTCGAC700.002592895615.85714331
GTCTATA700.002592895615.8571431
ACTACGC700.002592895615.8571433
CGCCTTA2250.015.62222225
TCTATAA957.061545E-515.5789482
TTTCGGA2400.015.41666730
CTTATAC13600.015.09926437
GTTTAGG2006.184564E-1114.81
GTTATAC1001.0936511E-414.83
TCTTATA21000.014.71190537
GCGCAAG3450.014.478261
Produced by FastQC (version 0.11.5)