FastQCFastQC Report
Fri 10 Feb 2017
ERR1630770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490290
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37610.2523669889753001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33730.22633178777284957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27810.18660797562890444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.12440531708593629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCG250.005496859629.63
TCTAGCG1500.020.96666728
GGTATCA18450.017.948511
CGCAATA1800.017.47222336
CTAGCGG1800.017.47222329
GCTATAC752.067785E-417.2666663
CAAGACG2950.016.3050844
AAGACGG3000.016.0333335
ACGGACC3000.014.88
TTATACC1152.2106035E-514.4782614
GACGGAC3100.014.322587
TCTTATA10750.014.2837237
GCCTAAG2101.364242E-1014.0952381
GCAATAC2101.364242E-1014.09523837
ATTGCCG2101.364242E-1014.09523811
GTTTATA2650.013.9622641
CGAATTA800.006299778413.87515
GGCGCAA3200.013.87534
CGGTGAT1753.56722E-813.74285714
GTCCTAT2700.013.7037041
Produced by FastQC (version 0.11.5)