##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630770.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1490290
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.961717518066955	33.0	31.0	34.0	30.0	34.0
2	32.1325346073583	33.0	31.0	34.0	30.0	34.0
3	32.23861127699978	34.0	31.0	34.0	30.0	34.0
4	35.83032362828711	37.0	35.0	37.0	35.0	37.0
5	35.799808091042685	37.0	35.0	37.0	35.0	37.0
6	35.82969422058794	37.0	35.0	37.0	35.0	37.0
7	35.80251293372431	37.0	35.0	37.0	35.0	37.0
8	35.79133255943474	37.0	35.0	37.0	35.0	37.0
9	37.517979722067516	39.0	37.0	39.0	35.0	39.0
10	37.40401935193821	39.0	37.0	39.0	34.0	39.0
11	37.51948614028142	39.0	37.0	39.0	35.0	39.0
12	37.44192338403935	39.0	37.0	39.0	35.0	39.0
13	37.49589207469687	39.0	37.0	39.0	35.0	39.0
14	38.76427071241168	40.0	38.0	41.0	35.0	41.0
15	38.73955404652786	40.0	38.0	41.0	35.0	41.0
16	38.72022156761436	40.0	38.0	41.0	35.0	41.0
17	38.698298988787414	40.0	38.0	41.0	35.0	41.0
18	38.679777761375306	40.0	38.0	41.0	34.0	41.0
19	38.70104006602742	40.0	38.0	41.0	34.0	41.0
20	38.68108488951815	40.0	38.0	41.0	34.0	41.0
21	38.61001550033886	40.0	38.0	41.0	34.0	41.0
22	38.552971569291884	40.0	38.0	41.0	34.0	41.0
23	38.53318817142972	40.0	38.0	41.0	34.0	41.0
24	38.48046152091204	40.0	38.0	41.0	34.0	41.0
25	38.43519180830577	40.0	38.0	41.0	34.0	41.0
26	38.24542941306726	40.0	38.0	41.0	34.0	41.0
27	38.13644726865241	40.0	38.0	41.0	33.0	41.0
28	38.03637144448396	40.0	38.0	41.0	33.0	41.0
29	37.93594669493857	40.0	37.0	41.0	33.0	41.0
30	37.8571459246187	40.0	37.0	41.0	33.0	41.0
31	37.820798636506986	40.0	37.0	41.0	33.0	41.0
32	37.729656644008884	40.0	37.0	41.0	33.0	41.0
33	37.69832918425273	40.0	37.0	41.0	33.0	41.0
34	37.627750303632176	40.0	37.0	41.0	32.0	41.0
35	37.571279415415795	40.0	37.0	41.0	32.0	41.0
36	37.52437377959994	40.0	37.0	41.0	32.0	41.0
37	37.352459588402255	40.0	36.0	41.0	31.0	41.0
38	37.24433969227466	40.0	36.0	41.0	31.0	41.0
39	37.185635681645856	39.0	36.0	41.0	31.0	41.0
40	37.09757228458891	39.0	36.0	41.0	31.0	41.0
41	36.97012460662019	39.0	36.0	41.0	31.0	41.0
42	36.89135336075529	39.0	35.0	41.0	31.0	41.0
43	36.015162820659064	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	3.0
13	1.0
14	1.0
15	8.0
16	6.0
17	19.0
18	47.0
19	107.0
20	221.0
21	442.0
22	783.0
23	1415.0
24	2268.0
25	3622.0
26	5533.0
27	8246.0
28	11597.0
29	16141.0
30	21987.0
31	28937.0
32	37543.0
33	48485.0
34	64474.0
35	86814.0
36	121953.0
37	186179.0
38	345804.0
39	497647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46375537647035	18.952351555737472	11.914929309060653	25.668963758731522
2	19.10386569057029	20.803534882472537	34.198713002167366	25.89388642478981
3	20.625381637131028	23.062222788853177	28.946044058538945	27.366351515476854
4	14.736326486791162	15.364660569419375	33.872266471626325	36.02674647216313
5	14.820135678290802	36.48987780901704	34.00076495178791	14.689221560904253
6	35.58676499204852	34.08652007327433	15.47920203450335	14.847512900173793
7	29.5022445295882	29.98188272081273	21.268947654483355	19.246925095115717
8	27.470089714082498	32.99894651376577	19.836407679042335	19.694556093109394
9	26.84960645243543	13.613323581316388	19.63624529453999	39.90082467170819
10	17.14867576109348	26.336216441095356	32.901113206154506	23.613994591656656
11	36.163162874339896	21.316522287608453	21.294848653617752	21.225466184433902
12	21.929825738614632	25.132155486516044	28.74319763267552	24.194821142193803
13	30.177884841205405	19.693415375530936	25.037475927504044	25.091223855759615
14	23.150729052734704	20.763072958954297	24.874823021022756	31.211374967288247
15	26.04949372269827	27.338974293593864	22.084292318944634	24.527239664763233
16	25.71855142287743	26.030571231102673	23.792818847338438	24.458058498681464
17	24.007743459326708	26.2431473069	25.260452663575546	24.488656570197747
18	24.074911594387668	24.80490374356669	26.598044675868454	24.522139986177187
19	24.583269028175724	25.183018070308467	26.35936629783465	23.87434660368116
20	24.922263452079797	24.56958041723423	26.21120721470318	24.296948915982792
21	25.213280636654613	25.03056452099927	25.60669399915453	24.149460843191594
22	25.136986760965986	24.911929892839648	25.680840641754287	24.270242704440076
23	24.50757906179334	25.006676552885683	25.959779640204257	24.52596474511672
24	24.552603855625417	25.479470438639463	25.651383287816465	24.31654241791866
25	24.978359916526312	24.89750317052386	25.699159224043644	24.424977688906186
26	24.464634400016106	25.751699333686734	25.663193069805207	24.120473196491957
27	25.09437760435888	25.002851793946146	25.293533473350827	24.609237128344148
28	24.21320682551718	25.148125532614458	26.22617074529118	24.412496896577178
29	23.96439619134531	25.420220225593678	26.34285944346402	24.27252413959699
30	23.940575324265748	25.91113139053473	26.219997450160708	23.928295835038817
31	24.453294325265553	25.7411644713445	25.441424152346187	24.364117051043756
32	23.878909473994995	25.417334881130515	25.85349160230559	24.850264042568895
33	23.8148950875333	25.219118426614955	26.268444396728153	24.697542089123594
34	24.539317850888082	25.01828503177234	26.002522998879414	24.439874118460164
35	24.421085828932622	25.094981513665125	26.520945587771504	23.962987069630742
36	23.760274845835376	25.53442618550752	26.013661770527886	24.691637198129225
37	24.390823262586476	24.773030752403894	26.313066584356065	24.523079400653565
38	23.775439679525462	24.69089908675493	26.969247596105454	24.564413637614155
39	23.968355152352895	24.665333592790663	26.708627179944845	24.657684074911597
40	23.54803427520818	24.597695750491518	27.3903736856585	24.46389628864181
41	22.88004348147005	24.65889189352408	27.7867394936556	24.674325131350276
42	23.46227915372176	24.587831898489558	27.51927477202424	24.430614175764447
43	22.916210938810565	24.18871494809735	27.683605204356198	25.211468908735885
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	72.0
1	96.0
2	120.0
3	326.5
4	533.0
5	533.0
6	790.0
7	1047.0
8	1058.0
9	1069.0
10	1578.0
11	2087.0
12	2087.0
13	3653.0
14	5219.0
15	7202.5
16	9186.0
17	9319.5
18	9453.0
19	9453.0
20	11678.0
21	13903.0
22	14297.5
23	14692.0
24	16881.5
25	19071.0
26	19071.0
27	21857.5
28	24644.0
29	29039.0
30	33434.0
31	37998.0
32	42562.0
33	42562.0
34	48040.5
35	53519.0
36	59096.0
37	64673.0
38	71077.5
39	77482.0
40	77482.0
41	81387.5
42	85293.0
43	86751.5
44	88210.0
45	90872.0
46	93534.0
47	93534.0
48	95994.0
49	98454.0
50	100688.0
51	102922.0
52	106343.5
53	109765.0
54	109765.0
55	105605.0
56	101445.0
57	97690.0
58	93935.0
59	87727.5
60	81520.0
61	81520.0
62	76111.0
63	70702.0
64	63765.5
65	56829.0
66	50113.0
67	43397.0
68	43397.0
69	37718.0
70	32039.0
71	27798.0
72	23557.0
73	18974.5
74	14392.0
75	14392.0
76	11431.0
77	8470.0
78	7083.0
79	5696.0
80	4445.0
81	3194.0
82	3194.0
83	2446.5
84	1699.0
85	1406.0
86	1113.0
87	969.5
88	826.0
89	826.0
90	639.5
91	453.0
92	254.5
93	56.0
94	36.0
95	16.0
96	16.0
97	11.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1490290.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.833784128079216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.35057909493754	54.25733646185935
2	8.333556041970978	10.472577227209094
3	2.2003646437913216	4.1477171109312545
4	0.9137252057734672	2.296512493278192
5	0.5089754385910114	1.5990426417461023
6	0.3209766804131378	1.2100907668335947
7	0.2269102967134825	0.9980342820093355
8	0.15180318168114942	0.7630694678167146
9	0.11430151799643856	0.6463797216569983
>10	0.7081088181651652	8.74274165270903
>50	0.09192578474681627	4.0503816680371845
>100	0.07535770608920603	8.846761208865853
>500	0.0028819033287547724	1.112842679604536
>1k	5.336858016212542E-4	0.8565126174428694
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3761	0.2523669889753001	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3373	0.22633178777284957	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2781	0.18660797562890444	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1854	0.12440531708593629	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.0130310208080307E-4	0.0
3	0.0	0.0	0.0	5.368082722154748E-4	0.0
4	0.0	0.0	0.0	0.001878828952754162	0.0
5	0.0	0.0	0.0	0.0024156372249696367	0.0
6	0.0	0.0	0.0	0.0024156372249696367	0.0
7	0.0	0.0	0.0	0.0033550517013467176	0.0
8	0.0	0.0	0.0	0.0038247589395352583	0.0
9	0.0	0.0	0.0	0.006643002368666501	0.0
10	0.0	0.0	0.0	0.009058639593636139	0.0
11	0.0	0.0	0.0	0.00986385200195935	0.0
12	0.0	0.0	0.0	0.011138771648471104	0.0
13	0.0	0.0	0.0	0.011675579920686577	0.0
14	0.0	0.0	0.0	0.012279489226928987	0.0
15	0.0	0.0	0.0	0.013286004737333003	0.0
16	0.0	0.0	0.0	0.015433237826194901	0.0
17	0.0	0.0	0.0	0.017983077119218406	0.0
18	0.0	0.0	0.0	0.01892249159559549	0.0
19	0.0	0.0	0.0	0.020264512276134176	0.0
20	0.0	0.0	0.0	0.02221044226291527	0.0
21	0.0	0.0	0.0	0.0240221701816425	0.0
22	0.0	0.0	0.0	0.02818243429131243	0.0
23	0.0	0.0	0.0	0.03314791080930557	0.0
24	0.0	0.0	0.0	0.042273651436968644	0.0
25	0.0	0.0	0.0	0.045226096934153755	0.0
26	0.0	0.0	0.0	0.0499902703500661	0.0
27	0.0	0.0	0.0	0.05958571821591771	0.0
28	0.0	0.0	0.0	0.08340658529547941	0.0
29	0.0	0.0	0.0	0.12044635607834717	0.0
30	0.0	0.0	0.0	0.17338907192559838	0.0
31	0.0	0.0	0.0	0.3681833737057888	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATCG	25	0.0054968596	29.6	3
TCTAGCG	150	0.0	20.966667	28
GGTATCA	1845	0.0	17.94851	1
CGCAATA	180	0.0	17.472223	36
CTAGCGG	180	0.0	17.472223	29
GCTATAC	75	2.067785E-4	17.266666	3
CAAGACG	295	0.0	16.305084	4
AAGACGG	300	0.0	16.033333	5
ACGGACC	300	0.0	14.8	8
TTATACC	115	2.2106035E-5	14.478261	4
GACGGAC	310	0.0	14.32258	7
TCTTATA	1075	0.0	14.28372	37
GCCTAAG	210	1.364242E-10	14.095238	1
GCAATAC	210	1.364242E-10	14.095238	37
ATTGCCG	210	1.364242E-10	14.095238	11
GTTTATA	265	0.0	13.962264	1
CGAATTA	80	0.0062997784	13.875	15
GGCGCAA	320	0.0	13.875	34
CGGTGAT	175	3.56722E-8	13.742857	14
GTCCTAT	270	0.0	13.703704	1
>>END_MODULE