FastQCFastQC Report
Fri 10 Feb 2017
ERR1630769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224096
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65970.5389283193475022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63600.5195670927770372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50070.4090365461532429No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37100.3030808041199383No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13550.11069393250202597No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT13220.10799806551120172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1001.2900273E-820.3499981
TAGGTTG701.21927966E-418.55
TCTATAC801.6168106E-518.53
GACGGAC2350.018.1063847
ACGGACC2400.017.7291668
TTTCGGA2150.017.20930330
CGGACCA2550.016.6862749
AAGACGG2800.016.5178575
CAAGACG2800.016.5178574
TACCAAC803.3827906E-416.18757
TGCACCG803.3827906E-416.18755
ACCGTCG1504.671165E-916.0333338
GTGCTAG2200.015.9772731
CCTAGTC700.00259262315.8571422
TAGACTA700.00259262315.8571425
AGACGGA2800.015.8571426
TATAGGG1059.3440085E-615.8571422
TCTTATA12450.015.75100337
TACGTTA855.3645036E-415.23529419
TTCGGAA2450.015.1020431
Produced by FastQC (version 0.11.5)