##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630769.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1224096
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.069188200925417	31.0	31.0	33.0	28.0	34.0
2	31.242075784905758	31.0	31.0	34.0	28.0	34.0
3	31.31721613337516	31.0	31.0	34.0	28.0	34.0
4	35.1080903785324	37.0	35.0	37.0	32.0	37.0
5	34.74616369957912	35.0	35.0	37.0	32.0	37.0
6	34.82752251457402	35.0	35.0	37.0	32.0	37.0
7	34.81718263927012	35.0	35.0	37.0	32.0	37.0
8	34.80528079497033	35.0	35.0	37.0	32.0	37.0
9	36.36906255718506	38.0	35.0	39.0	32.0	39.0
10	36.090589300185606	37.0	35.0	39.0	32.0	39.0
11	36.31952314197579	38.0	35.0	39.0	32.0	39.0
12	36.202504542127414	37.0	35.0	39.0	32.0	39.0
13	36.31310126003189	38.0	35.0	39.0	32.0	39.0
14	37.297380270828434	39.0	36.0	40.0	32.0	41.0
15	37.24568171123833	39.0	36.0	40.0	32.0	41.0
16	37.19812988523776	38.0	36.0	40.0	32.0	41.0
17	37.16824415732099	38.0	36.0	40.0	32.0	41.0
18	37.132991203304314	38.0	36.0	40.0	32.0	41.0
19	37.18425352259953	39.0	36.0	40.0	32.0	41.0
20	37.14842626722087	39.0	36.0	40.0	32.0	41.0
21	37.07679299662772	39.0	36.0	40.0	31.0	41.0
22	37.006930828954594	39.0	36.0	40.0	31.0	41.0
23	36.95544793872376	39.0	36.0	40.0	31.0	41.0
24	36.87927090685698	38.0	36.0	40.0	31.0	41.0
25	36.80120921888479	38.0	36.0	40.0	31.0	41.0
26	36.51849691527462	38.0	35.0	40.0	30.0	41.0
27	36.36881747836771	38.0	35.0	40.0	30.0	41.0
28	36.24493912242177	38.0	35.0	40.0	30.0	41.0
29	36.09343139753745	38.0	35.0	40.0	30.0	41.0
30	35.96846080699553	38.0	35.0	40.0	29.0	41.0
31	35.8896459101247	38.0	35.0	40.0	29.0	41.0
32	35.7664472394322	38.0	34.0	40.0	29.0	41.0
33	35.70885535121428	38.0	34.0	40.0	29.0	41.0
34	35.61648351109717	38.0	34.0	40.0	28.0	41.0
35	35.52806806002144	38.0	34.0	40.0	28.0	41.0
36	35.44604508143152	38.0	34.0	40.0	27.0	41.0
37	35.19518975635898	38.0	34.0	40.0	27.0	41.0
38	35.02143295950644	38.0	33.0	40.0	26.0	41.0
39	34.926343195304945	38.0	33.0	40.0	26.0	41.0
40	34.782945945416046	38.0	33.0	40.0	25.0	41.0
41	34.58927894544219	38.0	33.0	40.0	25.0	41.0
42	34.46679672182574	37.0	33.0	40.0	24.0	41.0
43	33.543329934907064	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	3.0
12	1.0
13	1.0
14	11.0
15	14.0
16	36.0
17	69.0
18	166.0
19	313.0
20	564.0
21	981.0
22	1670.0
23	2862.0
24	4480.0
25	6882.0
26	9910.0
27	14457.0
28	19541.0
29	25870.0
30	34019.0
31	43435.0
32	55342.0
33	70914.0
34	93414.0
35	126576.0
36	177723.0
37	233772.0
38	227605.0
39	73457.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.39920725171882	20.91069654667608	13.16906517135911	23.521031030245993
2	18.624846417274462	22.94060269782762	34.69580817190809	23.73874271298983
3	21.20789545917967	25.116167359422793	29.931230883852244	23.74470629754529
4	15.56503738268894	16.683740490941886	33.899628787284655	33.851593339084516
5	13.634469845502315	38.68136159255483	33.87896047368834	13.805208088254517
6	33.57824876480276	34.95779742765273	16.162866311138995	15.301087496405511
7	27.246147360991298	31.912856507986305	22.22693318171124	18.614062949311165
8	26.442697304786556	34.27508953546127	20.7220675502575	18.56014560949468
9	26.22661948082503	14.467329359788774	20.88365618382872	38.422394975557474
10	16.882744490628184	28.130391733981646	33.053943481557	21.932920293833163
11	33.80837777429221	22.936354664993594	22.081029592450268	21.174237968263927
12	20.579758450317623	27.14615520351345	29.569984707081797	22.704101639087128
13	28.821840770658508	20.78970930384545	26.952297858991454	23.436152066504587
14	22.341384989412592	22.211493216218336	26.523573314511278	28.923548479857793
15	25.160771704180064	28.342384910987374	24.203412150680993	22.29343123415157
16	24.095250699291558	27.376856063576717	25.670372258384965	22.857520978746766
17	22.540634067916244	27.75109141766659	26.79748973936685	22.91078477505032
18	21.70140250437874	25.913572138132956	29.01251209055499	23.37251326693331
19	23.021478707552347	26.608452278252688	29.033425482968656	21.33664353122631
20	23.35454082032782	25.377094606958934	29.19485073066165	22.073513842051604
21	23.42553198441952	26.434282932057616	27.841117036572292	22.299068046950566
22	23.271622487125192	26.083820223250463	27.910229263064334	22.734328026560007
23	22.35935743601809	26.650605834836483	28.28977465819674	22.700262070948686
24	23.346289833477112	26.870931691632027	27.922728282749066	21.86005019214179
25	22.96470211486681	26.095502313544035	28.325637858468617	22.614157713120537
26	22.841264083862704	26.862353933024863	28.06912202964473	22.2272599534677
27	23.14377303740883	26.329470891171937	27.60232857553656	22.924427495882675
28	22.173015841894752	26.667026115598773	28.684760018822054	22.475198023684417
29	22.231426293362613	27.083169947455097	28.461656602096568	22.223747157085718
30	21.72648223668732	27.8077046244739	28.509120199722897	21.95669293911589
31	22.559668522730245	27.07769652053434	28.076719472982507	22.28591548375291
32	21.69429521867566	27.1253235040389	28.129901576346956	23.05047970093849
33	21.87663385878232	26.892253548741273	28.47856704049356	22.75254555198285
34	22.418829895694454	26.78891198075968	28.134884845633024	22.657373277912843
35	22.55239785114893	26.9016482367396	28.299822889707997	22.24613102240347
36	22.041571902857292	27.186103050741117	28.13357775860717	22.638747287794423
37	22.76741366690194	26.13561354664994	28.028030481269443	23.06894230517868
38	22.126205787781352	26.421212061799075	28.790143910281547	22.662438240138027
39	22.210186129192483	26.307658876427993	28.644567092777034	22.83758790160249
40	21.834643688076753	26.027860559956085	29.334954121245392	22.802541630721773
41	21.176607063498288	26.64317177737694	29.376617520194493	22.80360363893028
42	22.035363239484486	25.879914647217213	29.08186939586438	23.002852717433928
43	21.289425012417325	25.953193213604166	29.059322144668393	23.69805962931012
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	373.0
1	320.0
2	267.0
3	597.5
4	928.0
5	928.0
6	1444.5
7	1961.0
8	2058.5
9	2156.0
10	3268.5
11	4381.0
12	4381.0
13	7366.0
14	10351.0
15	14664.0
16	18977.0
17	18132.0
18	17287.0
19	17287.0
20	19043.0
21	20799.0
22	19647.5
23	18496.0
24	19753.5
25	21011.0
26	21011.0
27	23166.5
28	25322.0
29	31800.0
30	38278.0
31	40253.5
32	42229.0
33	42229.0
34	45394.0
35	48559.0
36	51824.0
37	55089.0
38	62140.5
39	69192.0
40	69192.0
41	73229.5
42	77267.0
43	74522.0
44	71777.0
45	75442.5
46	79108.0
47	79108.0
48	82071.0
49	85034.0
50	86438.5
51	87843.0
52	89819.5
53	91796.0
54	91796.0
55	82212.0
56	72628.0
57	67902.0
58	63176.0
59	57026.0
60	50876.0
61	50876.0
62	46862.0
63	42848.0
64	37928.0
65	33008.0
66	28640.5
67	24273.0
68	24273.0
69	20752.0
70	17231.0
71	15127.5
72	13024.0
73	10340.5
74	7657.0
75	7657.0
76	5955.0
77	4253.0
78	3513.0
79	2773.0
80	2192.0
81	1611.0
82	1611.0
83	1246.5
84	882.0
85	739.5
86	597.0
87	526.5
88	456.0
89	456.0
90	355.0
91	254.0
92	152.5
93	51.0
94	30.5
95	10.0
96	10.0
97	7.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1224096.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.192723622558084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.13871003778301	49.527541736019266
2	7.052965573472009	7.926506903790588
3	1.7509537974416556	2.9517258844652248
4	0.7859530443894635	1.7665936881474096
5	0.4453373083552091	1.2512358143609097
6	0.29381076776125387	0.9906016362083843
7	0.19470578351499165	0.7658733796540086
8	0.13561155785317786	0.6096306232374535
9	0.11438075764137329	0.578462967169244
>10	0.81722393658702	9.227844196115614
>50	0.13329998288012584	5.3514242824263665
>100	0.12858232917125317	13.924664525273847
>500	0.006567767960786102	2.4868134806697886
>1k	0.0014595039912858006	1.1677902892704017
>5k	4.3785119738574014E-4	1.4732905931914808
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6597	0.5389283193475022	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6360	0.5195670927770372	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5007	0.4090365461532429	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3710	0.3030808041199383	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1355	0.11069393250202597	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1322	0.10799806551120172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0010620082085065223	0.0
3	0.0	0.0	0.0	0.001225394086738295	0.0
4	0.0	0.0	0.0	0.0018789375996653857	0.0
5	0.0	0.0	0.0	0.0021240164170130446	0.0
6	0.0	0.0	0.0	0.002205709356128931	0.0
7	0.0	0.0	0.0	0.0029409458081719082	0.0
8	0.0	0.0	0.0	0.0029409458081719082	0.0
9	0.0	0.0	0.0	0.005391733981648498	0.0
10	0.0	0.0	0.0	0.00890453036363161	0.0
11	0.0	8.169293911588634E-5	0.0	0.010211617389485792	0.0
12	0.0	8.169293911588634E-5	0.0	0.011191932658876427	0.0
13	0.0	8.169293911588634E-5	0.0	0.012090554989151178	0.0
14	0.0	8.169293911588634E-5	0.0	0.013479334954121245	0.0
15	0.0	8.169293911588634E-5	0.0	0.016420280762293155	0.0
16	0.0	8.169293911588634E-5	0.0	0.019361226570465063	0.0
17	0.0	8.169293911588634E-5	0.0	0.023037408830679947	0.0
18	0.0	8.169293911588634E-5	0.0	0.025569889943272424	0.0
19	0.0	8.169293911588634E-5	0.0	0.028674221629676103	0.0
20	0.0	8.169293911588634E-5	0.0	0.03218701801165921	0.0
21	0.0	8.169293911588634E-5	0.0	0.0370885943586124	0.0
22	0.0	8.169293911588634E-5	0.0	0.046074817661359894	0.0
23	0.0	8.169293911588634E-5	0.0	0.058737223224322274	0.0
24	0.0	8.169293911588634E-5	0.0	0.08005908033356861	0.0
25	0.0	8.169293911588634E-5	0.0	0.08528742843698534	0.0
26	0.0	8.169293911588634E-5	0.0	0.09517227407000758	0.0
27	0.0	8.169293911588634E-5	0.0	0.11330810655373434	0.0
28	0.0	8.169293911588634E-5	0.0	0.14957977152118787	0.0
29	0.0	8.169293911588634E-5	0.0	0.19344887982641884	0.0
30	0.0	8.169293911588634E-5	0.0	0.2692599273259614	0.0
31	0.0	8.169293911588634E-5	0.0	0.5051074425535252	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	100	1.2900273E-8	20.349998	1
TAGGTTG	70	1.21927966E-4	18.5	5
TCTATAC	80	1.6168106E-5	18.5	3
GACGGAC	235	0.0	18.106384	7
ACGGACC	240	0.0	17.729166	8
TTTCGGA	215	0.0	17.209303	30
CGGACCA	255	0.0	16.686274	9
AAGACGG	280	0.0	16.517857	5
CAAGACG	280	0.0	16.517857	4
TACCAAC	80	3.3827906E-4	16.1875	7
TGCACCG	80	3.3827906E-4	16.1875	5
ACCGTCG	150	4.671165E-9	16.033333	8
GTGCTAG	220	0.0	15.977273	1
CCTAGTC	70	0.002592623	15.857142	2
TAGACTA	70	0.002592623	15.857142	5
AGACGGA	280	0.0	15.857142	6
TATAGGG	105	9.3440085E-6	15.857142	2
TCTTATA	1245	0.0	15.751003	37
TACGTTA	85	5.3645036E-4	15.235294	19
TTCGGAA	245	0.0	15.10204	31
>>END_MODULE