FastQCFastQC Report
Fri 10 Feb 2017
ERR1630768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697558
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62200.36640868824511447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50530.2976628780872288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47390.27916571922726646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33980.20016989110239533No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG27850.16405919562100382No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG22840.1345462128539938No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT22260.13112954019833195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTCG2050.017.1463419
CAAGACG2650.016.7547154
ATACCGT2550.016.6862746
TCGGTGA2050.016.24390213
CGGTGAT2200.015.97727314
AAGACGG2800.015.8571435
GGTATCA32950.015.7769361
GTCGGTG2003.6379788E-1215.72512
AGTCGGT2003.6379788E-1215.72511
TAGACTA957.062848E-515.5789485
TACCGTC2550.015.2352947
GTATAGG1353.975092E-715.0740751
CTTATAC10550.014.72985737
TCACGTA1651.6463673E-814.57575725
CAGTCGG2450.014.34693810
GTCCTAT4000.014.3375011
ACCGTCG2750.014.1272748
CGCCTTA2101.364242E-1014.09523825
ACGGACC2900.014.0344838
AGACCGA800.00630025213.8750016
Produced by FastQC (version 0.11.5)