##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630768.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1697558
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.938375006921707	33.0	31.0	34.0	30.0	34.0
2	32.11205920504631	33.0	31.0	34.0	30.0	34.0
3	32.2082603363184	34.0	31.0	34.0	30.0	34.0
4	35.8007938462191	37.0	35.0	37.0	35.0	37.0
5	35.775976432027655	37.0	35.0	37.0	35.0	37.0
6	35.81562338370765	37.0	35.0	37.0	35.0	37.0
7	35.78958774899002	37.0	35.0	37.0	35.0	37.0
8	35.784459794599066	37.0	35.0	37.0	35.0	37.0
9	37.49881594620037	39.0	37.0	39.0	35.0	39.0
10	37.404936974171136	39.0	37.0	39.0	34.0	39.0
11	37.50493650290594	39.0	37.0	39.0	35.0	39.0
12	37.44108949443848	39.0	37.0	39.0	35.0	39.0
13	37.4866378645089	39.0	37.0	39.0	35.0	39.0
14	38.75585870998222	40.0	38.0	41.0	35.0	41.0
15	38.73106485905047	40.0	38.0	41.0	35.0	41.0
16	38.70198485118034	40.0	38.0	41.0	35.0	41.0
17	38.687899323616634	40.0	38.0	41.0	35.0	41.0
18	38.66375051691901	40.0	38.0	41.0	34.0	41.0
19	38.70158133035808	40.0	38.0	41.0	34.0	41.0
20	38.68151426932099	40.0	38.0	41.0	34.0	41.0
21	38.611865986316815	40.0	38.0	41.0	34.0	41.0
22	38.55932521893214	40.0	38.0	41.0	34.0	41.0
23	38.53507803562529	40.0	38.0	41.0	34.0	41.0
24	38.48808229232816	40.0	38.0	41.0	34.0	41.0
25	38.437932017639454	40.0	38.0	41.0	34.0	41.0
26	38.24952726210238	40.0	38.0	41.0	34.0	41.0
27	38.12917732413266	40.0	38.0	41.0	33.0	41.0
28	38.03802049767961	40.0	37.0	41.0	33.0	41.0
29	37.93551737260229	40.0	37.0	41.0	33.0	41.0
30	37.86223622403476	40.0	37.0	41.0	33.0	41.0
31	37.8078598787199	40.0	37.0	41.0	33.0	41.0
32	37.70793162884567	40.0	37.0	41.0	33.0	41.0
33	37.66846788150979	40.0	37.0	41.0	33.0	41.0
34	37.592758539030775	40.0	37.0	41.0	32.0	41.0
35	37.53094562895642	40.0	37.0	41.0	32.0	41.0
36	37.479021040812746	40.0	37.0	41.0	32.0	41.0
37	37.28715189701913	40.0	36.0	41.0	31.0	41.0
38	37.16357732696026	40.0	36.0	41.0	31.0	41.0
39	37.09125520306228	39.0	36.0	41.0	31.0	41.0
40	36.98449714236568	39.0	36.0	41.0	31.0	41.0
41	36.857455827724294	39.0	35.0	41.0	31.0	41.0
42	36.756827159955655	39.0	35.0	41.0	30.0	41.0
43	35.90087113371089	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	6.0
12	1.0
13	2.0
14	2.0
15	4.0
16	14.0
17	27.0
18	84.0
19	135.0
20	258.0
21	494.0
22	948.0
23	1630.0
24	2695.0
25	4271.0
26	6641.0
27	9664.0
28	13753.0
29	19127.0
30	25845.0
31	33669.0
32	43191.0
33	56156.0
34	74122.0
35	98739.0
36	138407.0
37	212361.0
38	388113.0
39	567194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.659715897777865	19.24918029310339	12.130130457987297	24.96097335113145
2	18.936260204364153	21.51973599723839	34.39811776681563	25.14588603158184
3	21.046350109981514	23.948990255413953	29.13820912157346	25.86645051303107
4	15.080309479852824	15.512459662644812	33.5368806249919	35.87035023251047
5	14.351026592316728	37.32644186531476	33.92078503355997	14.401746508808536
6	35.13376273446916	33.74983358447841	15.74579484176682	15.37060883928561
7	28.52373821689745	30.531681391740374	21.684619906948686	19.259960484413494
8	26.915015569423844	33.20870332560066	20.347935092644846	19.528346012330655
9	26.59054948343444	14.065911150016671	20.08131680920475	39.26222255734414
10	17.31481339665567	27.051741383799556	32.44024651882292	23.193198700721858
11	34.99951106236134	21.981988244289738	21.61045454706113	21.408046146287784
12	21.459649685018125	26.173420878697517	28.31720624567761	24.04972319060674
13	29.383973920184168	20.145880140766913	26.06355718037322	24.4065887586757
14	22.996445482275128	21.62447468657919	25.028128641259972	30.350951189885706
15	25.995577176155393	27.81183323338584	22.855596097452928	23.336993493005835
16	25.575503163956697	26.3999227125082	24.130662987656386	23.893911135878714
17	23.866224305738008	26.67891170728776	25.33851567958208	24.116348307392148
18	23.023307598326536	24.98159120336389	27.499620042437428	24.495481155872138
19	24.10415432050039	25.753346866498816	27.184343627728776	22.95815518527202
20	24.410947961719128	24.62708196126436	27.48642461700867	23.475545460007847
21	24.525583220131505	25.774200351328204	26.175423755771526	23.524792672768765
22	24.44982734021459	25.138640329225865	26.400511794000558	24.011020536558984
23	23.43554682667691	25.76495177189822	26.84933298302621	23.950168418398665
24	24.499899267064805	26.030156259756666	26.31780475247385	23.152139720704685
25	24.338078581114754	25.327323131227324	26.62854523969137	23.70605304796655
26	24.21342893733233	25.986858770068533	26.413000321638496	23.38671197096064
27	24.536952492933967	25.14011303295675	26.101081671436265	24.221852802673016
28	23.220826622713332	25.662157051482186	27.22781784186461	23.88919848393987
29	23.18224178496405	26.227027294501866	26.861526969917964	23.72920395061612
30	22.86826134953857	26.863353122544265	26.966619108154184	23.301766419762977
31	23.780630764898756	26.221607744772196	26.42165981957612	23.576101670752927
32	22.9753563648488	26.019258252148088	26.543246239598297	24.46213914340482
33	23.02825588286232	25.835464826533173	26.90776986706787	24.228509423536636
34	23.730087572854654	25.812903005375958	26.691046786030288	23.7659626357391
35	23.815268756649257	25.730254871998483	27.085201212565345	23.369275158786916
36	23.088106562485642	26.372530423113673	26.6860985014945	23.853264512906186
37	24.146273647203806	25.025831223439788	26.707482159667006	24.1204129696894
38	23.21122459438794	25.267590267902477	27.6574938823887	23.86369125532088
39	23.389480653974708	25.170509637962297	27.33326342899624	24.106746279066755
40	22.972234232939318	24.820889772249313	28.227371318093404	23.979504676717966
41	22.295674138969037	25.40708476529226	28.474078647091883	23.82316244864682
42	23.266185897624705	24.727166906815555	28.211701750396745	23.794945445162995
43	22.296911210102984	24.707550493120117	28.196621264192444	24.798917032584452
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	220.0
1	221.5
2	223.0
3	615.5
4	1008.0
5	1008.0
6	1432.0
7	1856.0
8	1897.0
9	1938.0
10	2906.0
11	3874.0
12	3874.0
13	6571.5
14	9269.0
15	12489.0
16	15709.0
17	15528.5
18	15348.0
19	15348.0
20	17707.5
21	20067.0
22	19783.5
23	19500.0
24	21164.0
25	22828.0
26	22828.0
27	25298.0
28	27768.0
29	35610.5
30	43453.0
31	46308.5
32	49164.0
33	49164.0
34	54392.0
35	59620.0
36	64732.0
37	69844.0
38	81194.5
39	92545.0
40	92545.0
41	97735.0
42	102925.0
43	99449.0
44	95973.0
45	102211.5
46	108450.0
47	108450.0
48	114511.0
49	120572.0
50	123950.5
51	127329.0
52	132465.0
53	137601.0
54	137601.0
55	123668.0
56	109735.0
57	103260.0
58	96785.0
59	88922.5
60	81060.0
61	81060.0
62	76448.0
63	71836.0
64	64401.5
65	56967.0
66	50442.5
67	43918.0
68	43918.0
69	37866.5
70	31815.0
71	27722.0
72	23629.0
73	18549.0
74	13469.0
75	13469.0
76	10589.0
77	7709.0
78	6560.0
79	5411.0
80	4362.5
81	3314.0
82	3314.0
83	2533.0
84	1752.0
85	1543.5
86	1335.0
87	1197.5
88	1060.0
89	1060.0
90	824.5
91	589.0
92	327.5
93	66.0
94	42.0
95	18.0
96	18.0
97	11.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1697558.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.344230073380956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.40899175146146	43.501941611444536
2	7.934442442790648	7.9890679168770244
3	2.0069313069898023	3.0311223438169725
4	0.9178620935077885	1.8483624164476484
5	0.534062559028652	1.3443484172658524
6	0.34314596620399873	1.036525168279603
7	0.25000496216033635	0.8810415134140812
8	0.18364809193904744	0.7396497434493459
9	0.144005040638564	0.6524840607870998
>10	0.9642246773529759	9.58495179839531
>50	0.13679062895553357	4.873805710731069
>100	0.15969785922176077	16.89595053847062
>500	0.012320471548548198	4.176566817616304
>1k	0.0036374725524285154	2.7782539956339622
>5k	2.3467564854377521E-4	0.6659279473704929
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6220	0.36640868824511447	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5053	0.2976628780872288	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4739	0.27916571922726646	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3398	0.20016989110239533	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2785	0.16405919562100382	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2284	0.1345462128539938	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2226	0.13112954019833195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1781629847109789E-4	0.0
2	0.0	0.0	0.0	9.425303877687831E-4	0.0
3	0.0	5.8908149235548946E-5	0.0	0.0017083363278309195	0.0
4	0.0	5.8908149235548946E-5	0.0	0.00335776450642629	0.0
5	0.0	1.1781629847109789E-4	0.0	0.0038879378495462304	0.0
6	0.0	1.1781629847109789E-4	0.0	0.004064662297252877	0.0
7	0.0	1.1781629847109789E-4	0.0	0.005125008983492758	0.0
8	5.8908149235548946E-5	1.1781629847109789E-4	0.0	0.005714090475848248	0.0
9	5.8908149235548946E-5	1.1781629847109789E-4	0.0	0.00954312017615893	0.0
10	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.013784506921118454	0.0
11	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.016258649189011508	0.0
12	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.017554628472193586	0.0
13	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.01849715885996237	0.0
14	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.019498597396966702	0.0
15	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.02167819891868201	0.0
16	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.02403452488810397	0.0
17	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.027038840499116968	0.0
18	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.029218442020832278	0.0
19	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.032222757631845275	0.0
20	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.03510925694438717	0.0
21	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.03893828664469785	0.0
22	1.1781629847109789E-4	1.1781629847109789E-4	0.0	0.044475652672839455	0.0
23	1.1781629847109789E-4	1.7672444770664683E-4	0.0	0.05101445723798539	0.0
24	1.1781629847109789E-4	1.7672444770664683E-4	0.0	0.06420988266674835	0.0
25	1.1781629847109789E-4	1.7672444770664683E-4	0.0	0.06898144275482782	0.0
26	1.1781629847109789E-4	1.7672444770664683E-4	0.0	0.07693404290162692	0.0
27	1.1781629847109789E-4	1.7672444770664683E-4	0.0	0.09089527427045202	0.0
28	1.1781629847109789E-4	1.7672444770664683E-4	0.0	0.122764583006884	0.0
29	1.1781629847109789E-4	1.7672444770664683E-4	0.0	0.16400028747176826	0.0
30	1.1781629847109789E-4	1.7672444770664683E-4	0.0	0.22508803822903253	0.0
31	1.1781629847109789E-4	1.7672444770664683E-4	0.0	0.4302651220164495	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGTCG	205	0.0	17.146341	9
CAAGACG	265	0.0	16.754715	4
ATACCGT	255	0.0	16.686274	6
TCGGTGA	205	0.0	16.243902	13
CGGTGAT	220	0.0	15.977273	14
AAGACGG	280	0.0	15.857143	5
GGTATCA	3295	0.0	15.776936	1
GTCGGTG	200	3.6379788E-12	15.725	12
AGTCGGT	200	3.6379788E-12	15.725	11
TAGACTA	95	7.062848E-5	15.578948	5
TACCGTC	255	0.0	15.235294	7
GTATAGG	135	3.975092E-7	15.074075	1
CTTATAC	1055	0.0	14.729857	37
TCACGTA	165	1.6463673E-8	14.575757	25
CAGTCGG	245	0.0	14.346938	10
GTCCTAT	400	0.0	14.337501	1
ACCGTCG	275	0.0	14.127274	8
CGCCTTA	210	1.364242E-10	14.095238	25
ACGGACC	290	0.0	14.034483	8
AGACCGA	80	0.006300252	13.875001	6
>>END_MODULE