FastQCFastQC Report
Fri 10 Feb 2017
ERR1630767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865051
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53340.6166110437419297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42680.4933813150900929No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39260.453846073815301No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31580.3650651811280491No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.10438690897993297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG1059.822543E-1021.1428575
AAGACGG2100.019.380955
GGTATCA22600.019.3185841
TACGCTA609.234295E-418.59
CGGACCA2250.018.0888889
GCGCAAG2300.017.695651
ACGGACC2300.017.695658
GACGGAC2300.017.695657
CGCAAGA2350.017.3191492
AGCGTCA650.001579506817.0769233
GGTTTAG1201.0402982E-716.9583341
AACCGCG1005.874408E-616.6500027
GCGAAAG2450.016.61224418
ATTGCCG1251.656208E-716.2811
GCAGTCG1151.2412493E-616.0869569
GAGCGAA2550.015.96078416
ATAAGAC957.0564485E-515.5789483
TTATACT1802.0190782E-1015.4166664
CGAAAGC2650.015.35849119
CCGCTAC1455.3394615E-815.3103461
Produced by FastQC (version 0.11.5)