##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630767.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865051
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.869255107502333	31.0	31.0	33.0	28.0	34.0
2	31.045123351108778	31.0	31.0	34.0	28.0	34.0
3	31.111614228525255	31.0	31.0	34.0	27.0	34.0
4	34.92632226308044	35.0	35.0	37.0	32.0	37.0
5	34.43673609995249	35.0	35.0	37.0	30.0	37.0
6	34.5356643712336	35.0	35.0	37.0	30.0	37.0
7	34.521778484736735	35.0	35.0	37.0	30.0	37.0
8	34.51188080240356	35.0	35.0	37.0	30.0	37.0
9	36.01907633191569	37.0	35.0	39.0	32.0	39.0
10	35.70878826797495	37.0	35.0	39.0	30.0	39.0
11	35.96727013783002	37.0	35.0	39.0	32.0	39.0
12	35.83051519505786	37.0	35.0	39.0	30.0	39.0
13	35.96572225221403	37.0	35.0	39.0	31.0	39.0
14	36.89038449756141	38.0	36.0	40.0	32.0	41.0
15	36.83024006677063	38.0	36.0	40.0	31.0	41.0
16	36.77864657690703	38.0	36.0	40.0	31.0	41.0
17	36.74792122082975	38.0	36.0	40.0	31.0	41.0
18	36.71883391846261	38.0	36.0	40.0	31.0	41.0
19	36.73391279820496	38.0	36.0	40.0	31.0	41.0
20	36.719159910802944	38.0	36.0	40.0	31.0	41.0
21	36.636489640495185	38.0	35.0	40.0	31.0	41.0
22	36.55420547459052	38.0	35.0	40.0	31.0	41.0
23	36.49527600106814	38.0	35.0	40.0	30.0	41.0
24	36.41770254008145	38.0	35.0	40.0	30.0	41.0
25	36.32966033216539	38.0	35.0	40.0	30.0	41.0
26	36.03882430053257	38.0	35.0	40.0	29.0	41.0
27	35.8705798848854	38.0	34.0	40.0	29.0	41.0
28	35.71836458197262	38.0	34.0	40.0	29.0	41.0
29	35.55188191216472	38.0	34.0	40.0	27.0	41.0
30	35.427577102390494	38.0	34.0	40.0	27.0	41.0
31	35.336877247699846	38.0	34.0	40.0	27.0	41.0
32	35.20622252329631	38.0	34.0	40.0	27.0	41.0
33	35.119775597045724	38.0	34.0	40.0	27.0	41.0
34	35.03678049039883	38.0	33.0	40.0	27.0	41.0
35	34.92697655976353	37.0	33.0	40.0	26.0	40.0
36	34.84513398631988	37.0	33.0	40.0	26.0	40.0
37	34.602735561255926	37.0	33.0	40.0	25.0	40.0
38	34.422689529287865	37.0	33.0	40.0	24.0	40.0
39	34.29511901610425	37.0	33.0	39.0	24.0	40.0
40	34.13252860235986	37.0	33.0	39.0	24.0	40.0
41	33.93604076522656	37.0	32.0	39.0	23.0	40.0
42	33.79950777468612	37.0	32.0	39.0	23.0	40.0
43	32.86918921543354	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	1.0
12	6.0
13	1.0
14	6.0
15	19.0
16	57.0
17	92.0
18	176.0
19	312.0
20	511.0
21	965.0
22	1655.0
23	2699.0
24	4151.0
25	6057.0
26	8849.0
27	12179.0
28	16212.0
29	21844.0
30	28168.0
31	35745.0
32	45436.0
33	58405.0
34	76260.0
35	100674.0
36	131445.0
37	152407.0
38	122674.0
39	38039.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.31542417730284	21.5654337143128	12.815776179670332	23.30336592871403
2	19.5149187735752	22.939456748792846	33.39490966428569	24.150714813346266
3	21.837093997926132	25.111005015889237	29.032392309817574	24.019508676367057
4	15.38406406096288	16.764329501960002	34.14353604585163	33.70807039122548
5	13.866928077072913	38.36502125308219	33.653391534140766	14.114659135704139
6	33.749224034189886	34.8893880245211	15.928540629396418	15.432847311892594
7	27.566467179391736	31.738244334727085	22.166785542124106	18.528502943757072
8	27.377807782431322	32.905574353419624	20.668145577543985	19.04847228660507
9	26.666751440088504	13.850512859935426	21.43862038191968	38.04411531805639
10	17.575033148334608	27.443468650981274	32.79344223635369	22.188055964330427
11	34.09533079552535	22.46237505071955	21.71744787301558	21.72484628073952
12	21.223488557322053	27.086842278663337	29.478608775667563	22.211060388347047
13	29.395954689376698	20.763978077593112	26.63322740508941	23.20683982794078
14	22.60386959843986	22.251636030708017	26.81969039975678	28.324803971095346
15	25.54080626460174	27.85038107579784	24.09696075722703	22.511851902373387
16	24.257991725343363	26.807089986601945	25.975578318503768	22.95933996955093
17	22.395673781083428	27.209378406591057	27.103604296162885	23.29134351616263
18	22.400413386031577	25.17077027828417	29.07215875133374	23.35665758435052
19	22.846283051519507	26.013379557968257	29.08314076279896	22.057196627713278
20	23.56589380279313	24.96141845972087	29.08140676098866	22.39128097649734
21	24.21417927960317	25.549245073411857	28.17151821106501	22.065057435919964
22	23.724034767892295	25.611553538461894	28.5302253855553	22.134186308090506
23	22.99321080491208	25.61444354147906	28.709983573222853	22.682362080386014
24	22.79981180300352	26.270705426616463	28.500053754056122	22.429429016323894
25	22.97089998161958	26.0541864005706	28.46468011712604	22.510233500683775
26	22.594505988664253	26.692299066760228	28.3406411876294	22.37255375694612
27	23.186263006458578	25.98482632815869	28.253941097114506	22.57496956826823
28	22.622596817991077	26.066671213604746	28.769055234893663	22.54167673351051
29	22.068872239902618	26.36931232956207	29.059558338178903	22.502257092356402
30	22.092223464281297	26.842579223652706	28.923843796492925	22.14135351557307
31	22.561097553785846	26.98707937451087	28.225850267787678	22.22597280391561
32	22.076964248350674	26.604211774797093	28.334861181595073	22.98396279525716
33	21.88148444426976	26.496703662558623	28.872864143270167	22.74894774990145
34	22.71831371791952	26.187588939842854	28.648599909138305	22.44549743309932
35	22.4816802708742	26.20111415396318	29.02245069943853	22.29475487572409
36	21.941596507026752	26.8555264371696	28.36422361224945	22.838653443554193
37	22.729758129867488	26.11718846634476	28.420405270903103	22.732648132884652
38	22.11372508672899	26.048637594777652	28.81552648340965	23.022110835083712
39	22.371975756342692	26.13718728722353	28.56224661898547	22.928590337448313
40	21.80553516497871	26.118806868034373	29.087302367143668	22.988355599843246
41	21.47919602428065	26.149556500136985	29.38959668273894	22.98165079284343
42	22.040434610213733	26.170364521860563	29.108341589108623	22.680859278817085
43	21.674329027999505	25.523350646378073	29.066494345420097	23.735825980202325
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	434.0
1	340.0
2	246.0
3	489.0
4	732.0
5	732.0
6	1113.5
7	1495.0
8	1524.0
9	1553.0
10	2284.0
11	3015.0
12	3015.0
13	5589.5
14	8164.0
15	10382.5
16	12601.0
17	12351.0
18	12101.0
19	12101.0
20	13973.5
21	15846.0
22	14564.0
23	13282.0
24	14469.0
25	15656.0
26	15656.0
27	17482.5
28	19309.0
29	21764.0
30	24219.0
31	27436.0
32	30653.0
33	30653.0
34	33314.0
35	35975.0
36	38898.0
37	41821.0
38	44783.0
39	47745.0
40	47745.0
41	48948.0
42	50151.0
43	50691.5
44	51232.0
45	51835.5
46	52439.0
47	52439.0
48	53329.0
49	54219.0
50	54027.5
51	53836.0
52	54829.5
53	55823.0
54	55823.0
55	54005.0
56	52187.0
57	50411.5
58	48636.0
59	45110.5
60	41585.0
61	41585.0
62	38254.5
63	34924.0
64	31026.0
65	27128.0
66	23418.5
67	19709.0
68	19709.0
69	16769.0
70	13829.0
71	12096.0
72	10363.0
73	8107.5
74	5852.0
75	5852.0
76	4473.5
77	3095.0
78	2563.5
79	2032.0
80	1628.5
81	1225.0
82	1225.0
83	971.5
84	718.0
85	610.0
86	502.0
87	456.5
88	411.0
89	411.0
90	332.0
91	253.0
92	148.5
93	44.0
94	26.5
95	9.0
96	9.0
97	5.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	865051.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.94450031679138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.83061164088534	62.62271133049374
2	5.839235907988773	8.05166403816303
3	1.2309266239103671	2.545968630364057
4	0.5178251108309926	1.4280477407091956
5	0.29602773989649067	1.0204742303536318
6	0.1935321766630531	0.8005787529153114
7	0.14554179499155964	0.7024014431631378
8	0.10695226609142833	0.5899016434737628
9	0.08188269873807538	0.5080825574178274
>10	0.616013759198847	8.61561230008056
>50	0.08584686911550224	4.1548227092670045
>100	0.05375556419150761	6.454492722935062
>500	0.0011759029532950975	0.5727160648505293
>1k	5.039584085550418E-4	1.3147568793090414
>5k	1.6798613618501394E-4	0.6177689565040897
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5334	0.6166110437419297	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4268	0.4933813150900929	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3926	0.453846073815301	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3158	0.3650651811280491	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	903	0.10438690897993297	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.248009654922079E-4	0.0
3	0.0	0.0	0.0	0.0016184016896113639	0.0
4	0.0	0.0	0.0	0.002427602534417046	0.0
5	0.0	0.0	0.0	0.002543202655103572	0.0
6	0.0	0.0	0.0	0.002543202655103572	0.0
7	1.1560012068652599E-4	0.0	0.0	0.003005603137849676	0.0
8	1.1560012068652599E-4	0.0	0.0	0.0032368033792227278	0.0
9	1.1560012068652599E-4	0.0	0.0	0.004970805189520618	0.0
10	2.3120024137305198E-4	0.0	0.0	0.007167207482564612	0.0
11	2.3120024137305198E-4	0.0	0.0	0.00867000905148945	0.0
12	2.3120024137305198E-4	0.0	0.0	0.009710410137668183	0.0
13	2.3120024137305198E-4	0.0	0.0	0.010057210499727761	0.0
14	2.3120024137305198E-4	0.0	0.0	0.01098201146521997	0.0
15	2.3120024137305198E-4	0.0	0.0	0.012022412551398704	0.0
16	2.3120024137305198E-4	0.0	0.0	0.014565615206502276	0.0
17	2.3120024137305198E-4	0.0	0.0	0.01849601930984416	0.0
18	2.3120024137305198E-4	1.1560012068652599E-4	0.0	0.019883220758082472	0.0
19	2.3120024137305198E-4	1.1560012068652599E-4	0.0	0.021848422809753413	0.0
20	2.3120024137305198E-4	1.1560012068652599E-4	0.0	0.02369802474073783	0.0
21	2.3120024137305198E-4	1.1560012068652599E-4	0.0	0.02774402896476624	0.0
22	2.3120024137305198E-4	1.1560012068652599E-4	0.0	0.035026836568017375	0.0
23	2.3120024137305198E-4	1.1560012068652599E-4	0.0	0.043696845619506826	0.0
24	3.46800362059578E-4	1.1560012068652599E-4	0.0	0.05907166167081478	0.0
25	3.46800362059578E-4	1.1560012068652599E-4	0.0	0.06346446625690277	0.0
26	3.46800362059578E-4	1.1560012068652599E-4	0.0	0.0746776779634958	0.0
27	3.46800362059578E-4	1.1560012068652599E-4	0.0	0.08912769304931155	0.0
28	3.46800362059578E-4	1.1560012068652599E-4	0.0	0.1187213239450622	0.0
29	3.46800362059578E-4	1.1560012068652599E-4	0.0	0.15895016594397324	0.0
30	3.46800362059578E-4	1.1560012068652599E-4	0.0	0.22299263280430864	0.0
31	3.46800362059578E-4	1.1560012068652599E-4	0.0	0.42217164074719293	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	105	9.822543E-10	21.142857	5
AAGACGG	210	0.0	19.38095	5
GGTATCA	2260	0.0	19.318584	1
TACGCTA	60	9.234295E-4	18.5	9
CGGACCA	225	0.0	18.088888	9
GCGCAAG	230	0.0	17.69565	1
ACGGACC	230	0.0	17.69565	8
GACGGAC	230	0.0	17.69565	7
CGCAAGA	235	0.0	17.319149	2
AGCGTCA	65	0.0015795068	17.076923	3
GGTTTAG	120	1.0402982E-7	16.958334	1
AACCGCG	100	5.874408E-6	16.650002	7
GCGAAAG	245	0.0	16.612244	18
ATTGCCG	125	1.656208E-7	16.28	11
GCAGTCG	115	1.2412493E-6	16.086956	9
GAGCGAA	255	0.0	15.960784	16
ATAAGAC	95	7.0564485E-5	15.578948	3
TTATACT	180	2.0190782E-10	15.416666	4
CGAAAGC	265	0.0	15.358491	19
CCGCTAC	145	5.3394615E-8	15.310346	1
>>END_MODULE