FastQCFastQC Report
Fri 10 Feb 2017
ERR1630766.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630766.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746912
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.4720770318323979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33910.45400261342701687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24730.33109656827042544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17560.23510132385073473No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC11890.1591887665481342No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT10580.1416498864658755No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA8840.11835396941005098No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA8800.11781843108692859No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG7470.1000117818431087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG400.001930557823.1255
TGTACCG855.174661E-821.7647085
TAGTACC759.258647E-619.7333344
CGTTATT1052.2562745E-819.380952
GCGAACT609.2326856E-418.523
TGGACTA500.00703263318.4999985
GGCAATT500.00703263318.4999982
GCGTTAT1002.871584E-718.4999981
CCGTGCG953.602967E-617.52631613
GTGCGTA852.7210475E-517.41176615
TGCGTAC852.7210475E-517.41176616
GAGTACG2050.017.1463431
CGAACGA650.001579233917.07692316
CCTAGAC650.001579233917.0769233
GGTATAC650.001579233917.0769233
GGTATCA14500.016.9689661
AAGACGG1757.2759576E-1216.9142865
GCGAAAG1655.4569682E-1116.81818218
TGACCCG1107.7985896E-716.81818213
GAGCGGT1107.7985896E-716.8181827
Produced by FastQC (version 0.11.5)