##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630766.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	746912
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.112122981020523	31.0	31.0	34.0	28.0	34.0
2	31.285511546206248	31.0	31.0	34.0	28.0	34.0
3	31.38304244676749	31.0	31.0	34.0	28.0	34.0
4	35.14811651171758	37.0	35.0	37.0	32.0	37.0
5	34.85327722676835	35.0	35.0	37.0	32.0	37.0
6	34.89706016237522	35.0	35.0	37.0	32.0	37.0
7	34.8876065721263	35.0	35.0	37.0	32.0	37.0
8	34.86748238078917	35.0	35.0	37.0	32.0	37.0
9	36.43931547491538	38.0	35.0	39.0	32.0	39.0
10	36.17662455550319	37.0	35.0	39.0	32.0	39.0
11	36.388008225868646	38.0	35.0	39.0	32.0	39.0
12	36.27321960284478	38.0	35.0	39.0	32.0	39.0
13	36.38456471445097	38.0	35.0	39.0	32.0	39.0
14	37.371597992802364	39.0	36.0	40.0	32.0	41.0
15	37.32997193779187	39.0	36.0	40.0	32.0	41.0
16	37.2936503898719	39.0	36.0	40.0	32.0	41.0
17	37.259393342187565	39.0	36.0	40.0	32.0	41.0
18	37.228744483955275	39.0	36.0	40.0	32.0	41.0
19	37.23740815517758	39.0	36.0	40.0	32.0	41.0
20	37.21480843794182	39.0	36.0	40.0	32.0	41.0
21	37.12717964097511	39.0	36.0	40.0	32.0	41.0
22	37.05020939548434	39.0	36.0	40.0	31.0	41.0
23	37.023216925153164	39.0	36.0	40.0	31.0	41.0
24	36.931157887408425	39.0	36.0	40.0	31.0	41.0
25	36.86406564628765	38.0	36.0	40.0	31.0	41.0
26	36.58221048798252	38.0	35.0	40.0	30.0	41.0
27	36.42884570069834	38.0	35.0	40.0	30.0	41.0
28	36.292748007797435	38.0	35.0	40.0	30.0	41.0
29	36.14781527141082	38.0	35.0	40.0	30.0	41.0
30	36.03772465832655	38.0	35.0	40.0	29.0	41.0
31	35.95238662653699	38.0	35.0	40.0	29.0	41.0
32	35.86552900475558	38.0	35.0	40.0	29.0	41.0
33	35.79493300415578	38.0	34.0	40.0	29.0	41.0
34	35.71288317767019	38.0	34.0	40.0	29.0	41.0
35	35.61859228396384	38.0	34.0	40.0	28.0	41.0
36	35.55492882695685	38.0	34.0	40.0	28.0	41.0
37	35.32454693457864	38.0	34.0	40.0	27.0	41.0
38	35.174793014438116	38.0	34.0	40.0	26.0	41.0
39	35.08249432329377	38.0	34.0	40.0	26.0	41.0
40	34.94626274581209	38.0	33.0	40.0	26.0	41.0
41	34.77279251103209	38.0	33.0	40.0	25.0	41.0
42	34.661085911057796	38.0	33.0	40.0	25.0	41.0
43	33.72920906345058	37.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	2.0
13	2.0
14	4.0
15	4.0
16	21.0
17	44.0
18	78.0
19	163.0
20	305.0
21	610.0
22	1084.0
23	1779.0
24	2742.0
25	4166.0
26	5988.0
27	8576.0
28	12005.0
29	15584.0
30	20129.0
31	25772.0
32	32716.0
33	42428.0
34	54973.0
35	73304.0
36	103912.0
37	140785.0
38	146919.0
39	52815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.009886037444836	20.408963840452422	12.691053296773916	23.890096825328822
2	19.63644980934836	21.79627586650101	33.13295274409837	25.434321580052266
3	21.519402553446724	23.842300029990145	29.150957542521745	25.487339874041385
4	15.254541364980078	15.782716036159547	34.08540765177156	34.87733494708881
5	14.423921425817232	37.58381174756866	33.75685489053596	14.235411936078146
6	35.192365365665566	34.83435799665824	15.146898162032475	14.826378475643716
7	28.82508247290176	30.791846964568787	21.290593804892676	19.092476757636774
8	28.347382288676577	32.287069962726534	19.858698213444153	19.506849535152735
9	26.63888115333533	13.548985690416007	20.347510817874127	39.46462233837453
10	17.54035281264727	27.243370035559742	32.40314789426331	22.813129257529667
11	35.65480270768176	22.00834904245748	21.252972237693328	21.08387601216743
12	22.513897219485028	26.03399061736858	28.46506683518272	22.987045327963667
13	30.664790497407996	19.885742898761833	25.293742770232637	24.15572383359753
14	22.842851634462964	21.140776958999187	25.73823957842423	30.27813182811362
15	25.916306070862433	28.23732916327492	22.596637890407436	23.249726875455206
16	24.867588149607986	26.450505548177027	24.62378432800651	24.058121974208476
17	23.491924082087316	26.6536084572212	25.770371877811577	24.08409558287991
18	23.17903581680305	25.002543807034833	27.77971166616683	24.038708709995287
19	24.41786984276595	25.427091812690115	27.71303714493809	22.442001199605844
20	25.020216571697873	24.753920140525256	26.80114926524142	23.424714022535454
21	25.039763720491838	25.014057880981966	26.8896469731374	23.0565314253888
22	24.75485733259072	25.25424681890236	27.074273810033844	22.916622038473072
23	24.108462576581978	25.02557195492909	26.70595732830641	24.160008140182512
24	23.63344008397241	25.898901075360953	27.042007626065722	23.42565121460092
25	23.90120924553361	25.832226554132216	26.567922325521614	23.698641874812562
26	23.866667023692216	26.10454779143996	26.795392228267858	23.233392956599975
27	24.317322522599717	25.089702669123003	26.76620538965768	23.826769418619598
28	23.47692900903989	25.646528640589523	27.153935135598307	23.72260721477229
29	23.678291418533913	26.168678505633864	27.190753395312967	22.96227668051926
30	23.78794289019322	25.930631721005952	26.764732659269097	23.516692729531723
31	23.173144895248704	26.514770146951715	27.0373216657384	23.27476329206118
32	22.94487168501778	25.931568913071416	26.77624673321623	24.34731266869457
33	22.541343558545048	26.206567841994772	27.2967899832912	23.955298616168974
34	24.223871085214856	25.201496294074804	27.012954672036333	23.561677948674006
35	23.478937277751598	25.408080202219267	27.270280836296646	23.842701683732486
36	22.72141831969496	26.260255558887796	27.291702369221543	23.726623752195707
37	23.40838010368022	25.212608714279593	27.697774302729105	23.681236879311083
38	23.321087357011265	25.109785356240096	27.56589799066021	24.003229296088428
39	23.14757294031961	24.95836189537723	27.815726618396813	24.078338545906345
40	22.45726404181483	24.92636348057067	28.312706182254406	24.303666295360095
41	21.747407994516088	25.17967310740757	28.81397005269697	24.258948845379376
42	22.438118546763206	25.05971252302815	28.842889122145582	23.659279808063065
43	22.409199477314594	24.735444068377532	28.571772846064864	24.283583608243006
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	262.0
1	208.5
2	155.0
3	333.0
4	511.0
5	511.0
6	719.0
7	927.0
8	904.0
9	881.0
10	1326.0
11	1771.0
12	1771.0
13	3252.5
14	4734.0
15	6722.5
16	8711.0
17	8332.5
18	7954.0
19	7954.0
20	9247.5
21	10541.0
22	9985.0
23	9429.0
24	10623.0
25	11817.0
26	11817.0
27	13350.5
28	14884.0
29	16704.5
30	18525.0
31	20799.5
32	23074.0
33	23074.0
34	25621.0
35	28168.0
36	30674.5
37	33181.0
38	35565.0
39	37949.0
40	37949.0
41	39440.0
42	40931.0
43	41503.5
44	42076.0
45	43154.0
46	44232.0
47	44232.0
48	46335.0
49	48438.0
50	48423.5
51	48409.0
52	48374.0
53	48339.0
54	48339.0
55	46841.5
56	45344.0
57	44314.5
58	43285.0
59	43028.5
60	42772.0
61	42772.0
62	39534.5
63	36297.0
64	33179.0
65	30061.0
66	25989.5
67	21918.0
68	21918.0
69	19295.5
70	16673.0
71	14269.5
72	11866.0
73	8829.5
74	5793.0
75	5793.0
76	4207.0
77	2621.0
78	2141.5
79	1662.0
80	1353.0
81	1044.0
82	1044.0
83	837.0
84	630.0
85	533.0
86	436.0
87	395.0
88	354.0
89	354.0
90	282.0
91	210.0
92	120.0
93	30.0
94	21.5
95	13.0
96	13.0
97	8.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	746912.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.47437375809547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.61179219555268	59.32750349102495
2	5.77941252655298	7.568068317314973
3	1.3046159948478457	2.5625674577237225
4	0.5708026230121015	1.494917771247824
5	0.3223617670200257	1.0553217409594629
6	0.20765176641328476	0.8157521619403286
7	0.16378555986071885	0.7506629873749738
8	0.10835413594256615	0.567553535595125
9	0.08999488994457273	0.5303123154494661
>10	0.6580692108305487	8.698448382180294
>50	0.10460416762235951	4.92622609741836
>100	0.07527348459822883	9.017111836874827
>500	0.002051048626343198	0.886992876379457
>1k	0.0012306291758059189	1.7985610285162859
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3526	0.4720770318323979	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3391	0.45400261342701687	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2473	0.33109656827042544	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1756	0.23510132385073473	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1189	0.1591887665481342	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1058	0.1416498864658755	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	884	0.11835396941005098	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	880	0.11781843108692859	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	747	0.1000117818431087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3388458078060066E-4	0.0
2	0.0	0.0	0.0	5.355383231224026E-4	0.0
3	0.0	0.0	0.0	6.694229039030033E-4	0.0
4	0.0	0.0	0.0	0.0010710766462448053	0.0
5	1.3388458078060066E-4	0.0	0.0	0.0010710766462448053	0.0
6	1.3388458078060066E-4	0.0	0.0	0.0010710766462448053	0.0
7	2.677691615612013E-4	0.0	0.0	0.0013388458078060065	0.0
8	2.677691615612013E-4	0.0	0.0	0.0014727303885866073	0.0
9	2.677691615612013E-4	0.0	0.0	0.0021421532924896106	0.0
10	2.677691615612013E-4	0.0	0.0	0.0033471145195150165	0.0
11	2.677691615612013E-4	0.0	0.0	0.0037487682618568183	0.0
12	4.01653742341802E-4	0.0	0.0	0.004284306584979221	0.0
13	4.01653742341802E-4	0.0	0.0	0.004418191165759822	0.0
14	4.01653742341802E-4	0.0	0.0	0.004819844908101624	0.0
15	4.01653742341802E-4	0.0	0.0	0.005890921554346429	0.0
16	4.01653742341802E-4	0.0	0.0	0.007363651942933036	0.0
17	4.01653742341802E-4	0.0	0.0	0.009371920654642046	0.0
18	4.01653742341802E-4	0.0	0.0	0.011380189366351056	0.0
19	4.01653742341802E-4	0.0	0.0	0.013120688916498865	0.0
20	4.01653742341802E-4	0.0	0.0	0.014727303885866072	0.0
21	4.01653742341802E-4	0.0	0.0	0.017538880082258686	0.0
22	4.01653742341802E-4	0.0	0.0	0.023295917055824514	0.0
23	4.01653742341802E-4	0.0	0.0	0.03132899190266055	0.0
24	4.01653742341802E-4	0.0	0.0	0.047127372434771435	0.0
25	4.01653742341802E-4	0.0	0.0	0.05087614069662825	0.0
26	4.01653742341802E-4	0.0	0.0	0.05957863844736729	0.0
27	4.01653742341802E-4	0.0	0.0	0.07443982691401396	0.0
28	4.01653742341802E-4	0.0	0.0	0.11246304785570456	0.0
29	4.01653742341802E-4	0.0	0.0	0.17445160875712265	0.0
30	4.01653742341802E-4	0.0	0.0	0.25384516516001887	0.0
31	4.01653742341802E-4	0.0	0.0	0.5074225611584765	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACCG	40	0.0019305578	23.125	5
TGTACCG	85	5.174661E-8	21.764708	5
TAGTACC	75	9.258647E-6	19.733334	4
CGTTATT	105	2.2562745E-8	19.38095	2
GCGAACT	60	9.2326856E-4	18.5	23
TGGACTA	50	0.007032633	18.499998	5
GGCAATT	50	0.007032633	18.499998	2
GCGTTAT	100	2.871584E-7	18.499998	1
CCGTGCG	95	3.602967E-6	17.526316	13
GTGCGTA	85	2.7210475E-5	17.411766	15
TGCGTAC	85	2.7210475E-5	17.411766	16
GAGTACG	205	0.0	17.146343	1
CGAACGA	65	0.0015792339	17.076923	16
CCTAGAC	65	0.0015792339	17.076923	3
GGTATAC	65	0.0015792339	17.076923	3
GGTATCA	1450	0.0	16.968966	1
AAGACGG	175	7.2759576E-12	16.914286	5
GCGAAAG	165	5.4569682E-11	16.818182	18
TGACCCG	110	7.7985896E-7	16.818182	13
GAGCGGT	110	7.7985896E-7	16.818182	7
>>END_MODULE