FastQCFastQC Report
Fri 10 Feb 2017
ERR1630765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186394
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT114540.35946590409095674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT105200.33015377257175355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92790.2912069254461313No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62050.1947342356281113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54150.019.3711911
CTTATAC23950.016.53027237
AAGACGG5550.016.3333345
TATACTG4700.016.1382985
CGGACCA5450.015.6146789
GACGGAC5300.015.0094347
TCTAGCG4200.014.9761928
ACGGACC5850.014.8632488
TCTATAC3250.014.8000013
TTAACGG1406.0039747E-714.53571435
CAAGACG6250.014.5044
CTAGCGG4500.014.38888729
TAATACT6100.014.2540984
CGAACGA3400.014.14705916
GTATTAG6550.014.1221381
GCGCAAG6050.014.0661161
GCGAAAG5850.013.91453118
GTACCGA800.00630185413.8756
TCTTATA36550.013.81805737
CGTTTAG2151.9826984E-1013.76744226
Produced by FastQC (version 0.11.5)