##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630765.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3186394
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.081969147569325	33.0	31.0	34.0	30.0	34.0
2	32.25855998975645	34.0	31.0	34.0	30.0	34.0
3	32.36684289513475	34.0	31.0	34.0	30.0	34.0
4	35.93365823561054	37.0	35.0	37.0	35.0	37.0
5	35.9102691004314	37.0	35.0	37.0	35.0	37.0
6	35.94463867305801	37.0	35.0	37.0	35.0	37.0
7	35.92417918185887	37.0	35.0	37.0	35.0	37.0
8	35.919707669547456	37.0	35.0	37.0	35.0	37.0
9	37.656291720358496	39.0	37.0	39.0	35.0	39.0
10	37.574272986956416	39.0	37.0	39.0	35.0	39.0
11	37.67059535010422	39.0	37.0	39.0	35.0	39.0
12	37.61404616001662	39.0	37.0	39.0	35.0	39.0
13	37.65376786423776	39.0	37.0	39.0	35.0	39.0
14	38.96199434219371	40.0	38.0	41.0	36.0	41.0
15	38.93982570893618	40.0	38.0	41.0	36.0	41.0
16	38.919844187504744	40.0	38.0	41.0	36.0	41.0
17	38.915009254976	40.0	38.0	41.0	36.0	41.0
18	38.89823857313314	40.0	38.0	41.0	35.0	41.0
19	38.93735018331066	40.0	38.0	41.0	35.0	41.0
20	38.918786565628736	40.0	38.0	41.0	35.0	41.0
21	38.854561614163224	40.0	38.0	41.0	35.0	41.0
22	38.803909685996146	40.0	38.0	41.0	35.0	41.0
23	38.78366266067536	40.0	38.0	41.0	35.0	41.0
24	38.73668479164849	40.0	38.0	41.0	35.0	41.0
25	38.69505779887861	40.0	38.0	41.0	35.0	41.0
26	38.52799967612292	40.0	38.0	41.0	34.0	41.0
27	38.42779455396916	40.0	38.0	41.0	34.0	41.0
28	38.34196210512573	40.0	38.0	41.0	34.0	41.0
29	38.23995902578275	40.0	38.0	41.0	34.0	41.0
30	38.17035683597195	40.0	38.0	41.0	34.0	41.0
31	38.12817153183191	40.0	38.0	41.0	33.0	41.0
32	38.02262651762462	40.0	37.0	41.0	33.0	41.0
33	37.99655409845738	40.0	37.0	41.0	33.0	41.0
34	37.933779689517365	40.0	37.0	41.0	33.0	41.0
35	37.8797788346325	40.0	37.0	41.0	33.0	41.0
36	37.830761042105905	40.0	37.0	41.0	33.0	41.0
37	37.65848605037544	40.0	37.0	41.0	33.0	41.0
38	37.554393461699966	40.0	37.0	41.0	33.0	41.0
39	37.49410399341701	40.0	37.0	41.0	32.0	41.0
40	37.39828250994698	40.0	37.0	41.0	32.0	41.0
41	37.28084442790188	40.0	36.0	41.0	32.0	41.0
42	37.19777811532409	40.0	36.0	41.0	31.0	41.0
43	36.378727803278565	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	7.0
11	7.0
12	5.0
13	8.0
14	13.0
15	6.0
16	9.0
17	34.0
18	88.0
19	199.0
20	380.0
21	739.0
22	1297.0
23	2195.0
24	3815.0
25	6098.0
26	9272.0
27	13966.0
28	20607.0
29	28960.0
30	39967.0
31	53292.0
32	70337.0
33	92773.0
34	126201.0
35	170810.0
36	245682.0
37	382176.0
38	706263.0
39	1211178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07822573103012	19.577522428174294	12.43185870924939	24.912393131546192
2	18.09173630128603	21.420577618461493	35.64901264564269	24.83867343460978
3	20.07975159380792	24.012065049080558	29.83761581273377	26.07056754437775
4	14.271869706006225	15.914792709250644	34.9777208970391	34.83561668770403
5	13.868059003374974	37.523357124071914	34.59644350322025	14.012140369332856
6	33.88360008209907	35.17082319386742	16.22533183278653	14.72024489124697
7	28.08786358498039	31.10544395953545	22.06848870541433	18.73820375006983
8	26.450338533150642	33.99488575486898	20.73280956466777	18.821966147312605
9	26.341563535457325	14.284987983281413	20.618856299628984	38.75459218163228
10	16.759572105646697	27.22406582487916	33.424523144344356	22.591838925129785
11	34.325447512140684	22.431124336789487	22.177608920930684	21.065819230139148
12	20.987266483680298	26.233290672779326	29.766563708066236	23.012879135474144
13	29.04226533190811	20.559039465929196	26.362245221400745	24.036449980761954
14	22.509677083248334	21.79560970802732	25.99756966652586	29.697143542198486
15	25.07144439764825	28.155589045171435	23.447476991232094	23.325489565948214
16	24.455136433222005	27.25999986191287	24.933545569066474	23.35131813579865
17	22.85150549492624	27.238816040954134	26.402541556380033	23.507136907739596
18	22.49015658452784	25.88044667420288	28.085635360849913	23.54376138041937
19	23.34626540220701	26.225068211903483	27.963271334304547	22.465395051584956
20	23.945186941727858	25.37143868586245	27.86861888391706	22.814755488492636
21	24.081328297756023	26.065828645170686	26.80129952541964	23.05154353165365
22	23.865410241169172	25.859859138574826	27.02597356133611	23.248757058919896
23	23.225031179446106	26.019977441584434	27.37702870392048	23.377962675048973
24	23.496435155225626	26.40928899564837	27.147301934412383	22.946973914713624
25	23.614782101648444	25.93834911815676	27.161204797649003	23.28566398254579
26	23.24561871507416	26.681006805812462	27.171686866093776	22.901687613019607
27	23.743736650269867	25.957524399054225	26.734609718697687	23.564129231978217
28	22.855742259117985	26.232537470256347	27.590028100730795	23.321692169894874
29	22.635901272723963	26.635751887556907	27.694691867986194	23.033654971732936
30	22.529166198530376	27.17322465457818	27.605280451821084	22.69232869507035
31	23.215114012893572	26.71584242250017	26.99364861972499	23.075394944881268
32	22.38718752294914	26.5100298330966	27.307326087106613	23.795456556847647
33	22.377113439204315	26.264831028429004	27.767030693630478	23.591024838736203
34	23.23934202738268	25.955892460254443	27.52117283675528	23.283592675607597
35	23.064944259874956	26.03544947674393	27.915034989395537	22.984571273985576
36	22.487928360397362	26.38480991365161	27.657188659029615	23.470073066921415
37	23.17208731876849	25.4134611099569	27.711450624122442	23.703000947152173
38	22.53914613195983	25.40348117652745	28.499551530664448	23.557821160848285
39	22.66345593168955	25.339176511128255	28.38804617382533	23.60932138335686
40	21.993576437816543	25.281399600928196	29.179599258597648	23.545424702657613
41	21.414708915469962	25.49772564221499	29.52528783320581	23.56227760910923
42	22.091178931419027	25.195032378293458	29.27123889889323	23.442549791394285
43	21.345539817109874	24.958840620463132	29.287777970960278	24.407841591466717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	880.0
1	765.5
2	651.0
3	1552.0
4	2453.0
5	2453.0
6	3248.5
7	4044.0
8	3958.5
9	3873.0
10	5816.5
11	7760.0
12	7760.0
13	12933.0
14	18106.0
15	24392.0
16	30678.0
17	31332.5
18	31987.0
19	31987.0
20	37142.0
21	42297.0
22	43037.0
23	43777.0
24	48981.5
25	54186.0
26	54186.0
27	60378.5
28	66571.0
29	79162.5
30	91754.0
31	101568.0
32	111382.0
33	111382.0
34	121874.5
35	132367.0
36	142876.0
37	153385.0
38	166718.5
39	180052.0
40	180052.0
41	187167.5
42	194283.0
43	190650.5
44	187018.0
45	193371.0
46	199724.0
47	199724.0
48	205055.0
49	210386.0
50	214251.0
51	218116.0
52	226774.0
53	235432.0
54	235432.0
55	218616.5
56	201801.0
57	191544.5
58	181288.0
59	164988.0
60	148688.0
61	148688.0
62	137549.5
63	126411.0
64	111493.5
65	96576.0
66	83578.5
67	70581.0
68	70581.0
69	60267.0
70	49953.0
71	43326.5
72	36700.0
73	29341.0
74	21982.0
75	21982.0
76	17132.0
77	12282.0
78	10138.0
79	7994.0
80	6281.5
81	4569.0
82	4569.0
83	3572.0
84	2575.0
85	2163.5
86	1752.0
87	1536.0
88	1320.0
89	1320.0
90	988.0
91	656.0
92	365.5
93	75.0
94	47.0
95	19.0
96	19.0
97	13.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3186394.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.764322125707906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.70202393176953	45.83884601173139
2	10.266536402991031	11.244798133774147
3	2.65304008756603	4.35875825903647
4	1.0682465134713952	2.3400718469364747
5	0.5320784080928784	1.4569456668466134
6	0.3321696467930243	1.0914627322413482
7	0.22825673162844454	0.8750227624782978
8	0.15627647070668038	0.684669998595952
9	0.1264652177374177	0.6233203729682758
>10	0.7254037804532975	7.532426079959962
>50	0.08914654285148668	3.4620114002098155
>100	0.10461665943690053	12.322907196986993
>500	0.011890870605692326	4.380249014532158
>1k	0.003618960618921297	2.610129854781
>5k	1.148876386959142E-4	0.48710679367758225
>10k+	1.148876386959142E-4	0.6912738752435542
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11454	0.35946590409095674	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10520	0.33015377257175355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9279	0.2912069254461313	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6205	0.1947342356281113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.138343845739102E-5	0.0
2	0.0	0.0	0.0	7.218190845199935E-4	0.0
3	0.0	0.0	0.0	0.0010356534690939036	0.0
4	0.0	0.0	0.0	0.0017260891151565062	0.0
5	0.0	0.0	0.0	0.0019457731843582431	0.0
6	0.0	0.0	0.0	0.001977156622815634	0.0
7	0.0	0.0	0.0	0.0022909910073895443	0.0
8	0.0	0.0	0.0	0.0026048253919634546	0.0
9	6.276687691478204E-5	0.0	0.0	0.0047388992070660435	0.0
10	6.276687691478204E-5	0.0	0.0	0.007940009929719927	0.0
11	6.276687691478204E-5	0.0	0.0	0.008818746206526876	0.0
12	6.276687691478204E-5	0.0	0.0	0.009571948729504261	0.0
13	6.276687691478204E-5	0.0	0.0	0.010262384375566863	0.0
14	6.276687691478204E-5	0.0	0.0	0.011329421283118158	0.0
15	6.276687691478204E-5	0.0	0.0	0.012616142259871189	0.0
16	6.276687691478204E-5	3.138343845739102E-5	0.0	0.014436381690399869	0.0
17	6.276687691478204E-5	3.138343845739102E-5	0.0	0.016790139574704196	0.0
18	6.276687691478204E-5	3.138343845739102E-5	0.0	0.018076860551457227	0.0
19	6.276687691478204E-5	3.138343845739102E-5	0.0	0.020179550928102426	0.0
20	6.276687691478204E-5	3.138343845739102E-5	0.0	0.021403505027940674	0.0
21	6.276687691478204E-5	3.138343845739102E-5	0.0	0.024730149504424124	0.0
22	6.276687691478204E-5	3.138343845739102E-5	0.0	0.030253634672924943	0.0
23	6.276687691478204E-5	6.276687691478204E-5	0.0	0.03756597583349705	0.0
24	6.276687691478204E-5	6.276687691478204E-5	0.0	0.05096670405480302	0.0
25	6.276687691478204E-5	6.276687691478204E-5	0.0	0.05507793449272124	0.0
26	6.276687691478204E-5	6.276687691478204E-5	0.0	0.06276687691478204	0.0
27	6.276687691478204E-5	6.276687691478204E-5	0.0	0.08078097058932449	0.0
28	6.276687691478204E-5	6.276687691478204E-5	0.0	0.1205437871148389	0.0
29	6.276687691478204E-5	9.415031537217306E-5	0.0	0.17847761450718272	0.0
30	6.276687691478204E-5	9.415031537217306E-5	0.0	0.2629618308344793	0.0
31	6.276687691478204E-5	9.415031537217306E-5	0.0	0.5644939075330923	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5415	0.0	19.371191	1
CTTATAC	2395	0.0	16.530272	37
AAGACGG	555	0.0	16.333334	5
TATACTG	470	0.0	16.138298	5
CGGACCA	545	0.0	15.614678	9
GACGGAC	530	0.0	15.009434	7
TCTAGCG	420	0.0	14.97619	28
ACGGACC	585	0.0	14.863248	8
TCTATAC	325	0.0	14.800001	3
TTAACGG	140	6.0039747E-7	14.535714	35
CAAGACG	625	0.0	14.504	4
CTAGCGG	450	0.0	14.388887	29
TAATACT	610	0.0	14.254098	4
CGAACGA	340	0.0	14.147059	16
GTATTAG	655	0.0	14.122138	1
GCGCAAG	605	0.0	14.066116	1
GCGAAAG	585	0.0	13.914531	18
GTACCGA	80	0.006301854	13.875	6
TCTTATA	3655	0.0	13.818057	37
CGTTTAG	215	1.9826984E-10	13.767442	26
>>END_MODULE