FastQCFastQC Report
Fri 10 Feb 2017
ERR1630764.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630764.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592244
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81820.3156338677994819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71900.27736586525033907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60430.23311848730289278No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39430.15210759480974786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA1500.023.43333215
GGTATCA34550.019.1157741
GTACCGA957.0651426E-515.5789486
TACCGCA4300.015.48837228
AAGACGG5100.015.2352945
ATTGCCG4550.015.04395711
TAGTTCG1853.0559022E-1015.07
ACGGACC5200.014.9423078
TACGCAG750.004106255214.7999995
GACGGAC5250.014.7999997
CGCAATA3800.014.60526436
CGAGCCG4950.014.57575815
GTATAAC1651.6478225E-814.5757571
TTAACGG1406.002738E-714.53571435
ATACCGC4600.014.47826127
TAACGGC1551.2119017E-714.32258136
TACCGTC3750.014.3066677
CAAGACG5950.014.3025214
TCTTATA20900.014.074162537
CTTATAC13700.014.04379737
Produced by FastQC (version 0.11.5)