##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630764.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2592244
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93330836140425	33.0	31.0	34.0	30.0	34.0
2	32.101983069495	33.0	31.0	34.0	30.0	34.0
3	32.2002789860831	34.0	31.0	34.0	30.0	34.0
4	35.80031123613364	37.0	35.0	37.0	35.0	37.0
5	35.772134490426055	37.0	35.0	37.0	35.0	37.0
6	35.80749381616854	37.0	35.0	37.0	35.0	37.0
7	35.78127097603466	37.0	35.0	37.0	35.0	37.0
8	35.76736449192283	37.0	35.0	37.0	33.0	37.0
9	37.48523518619389	39.0	37.0	39.0	35.0	39.0
10	37.3738760703082	39.0	37.0	39.0	34.0	39.0
11	37.48847639342593	39.0	37.0	39.0	35.0	39.0
12	37.41121206182751	39.0	37.0	39.0	34.0	39.0
13	37.46461135602976	39.0	37.0	39.0	35.0	39.0
14	38.72227614375807	40.0	38.0	41.0	35.0	41.0
15	38.700670152963994	40.0	38.0	41.0	35.0	41.0
16	38.68112453920233	40.0	38.0	41.0	34.0	41.0
17	38.6569856078363	40.0	38.0	41.0	34.0	41.0
18	38.63997717807429	40.0	38.0	41.0	34.0	41.0
19	38.671356940164586	40.0	38.0	41.0	34.0	41.0
20	38.649187730784604	40.0	38.0	41.0	34.0	41.0
21	38.58198919546154	40.0	38.0	41.0	34.0	41.0
22	38.52198982811803	40.0	38.0	41.0	34.0	41.0
23	38.49509999830263	40.0	38.0	41.0	34.0	41.0
24	38.44923394556994	40.0	38.0	41.0	34.0	41.0
25	38.40578086013508	40.0	38.0	41.0	34.0	41.0
26	38.21376729968321	40.0	38.0	41.0	34.0	41.0
27	38.0947476394969	40.0	38.0	41.0	33.0	41.0
28	38.00063921451839	40.0	37.0	41.0	33.0	41.0
29	37.897227652952424	40.0	37.0	41.0	33.0	41.0
30	37.82227174602391	40.0	37.0	41.0	33.0	41.0
31	37.78156222948149	40.0	37.0	41.0	33.0	41.0
32	37.678657178876676	40.0	37.0	41.0	33.0	41.0
33	37.64291787347179	40.0	37.0	41.0	33.0	41.0
34	37.57544582994502	40.0	37.0	41.0	32.0	41.0
35	37.520834458484615	40.0	37.0	41.0	32.0	41.0
36	37.47775209432445	40.0	37.0	41.0	32.0	41.0
37	37.303159347654	40.0	36.0	41.0	31.0	41.0
38	37.19149586227223	40.0	36.0	41.0	31.0	41.0
39	37.132137252511725	39.0	36.0	41.0	31.0	41.0
40	37.03006854293037	39.0	36.0	41.0	31.0	41.0
41	36.91328246877995	39.0	35.0	41.0	31.0	41.0
42	36.830042619444775	39.0	35.0	41.0	30.0	41.0
43	35.9641372494256	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	11.0
11	2.0
12	4.0
13	3.0
14	6.0
15	5.0
16	17.0
17	46.0
18	88.0
19	205.0
20	423.0
21	832.0
22	1474.0
23	2592.0
24	4172.0
25	6748.0
26	9976.0
27	14753.0
28	21228.0
29	29401.0
30	39362.0
31	51438.0
32	66445.0
33	85824.0
34	113193.0
35	151206.0
36	211528.0
37	326315.0
38	603563.0
39	851379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2830397138541	19.234724817571188	12.214938099962811	25.267297368611906
2	18.80447982520164	20.99408851944493	34.84965149885581	25.351780156497618
3	20.348084516735305	23.311308657672658	29.512229558637227	26.828377266954806
4	14.618531280234423	15.480255716668648	34.32485522196213	35.576357781134796
5	14.424838093944862	36.93641493624828	34.217727960793816	14.421019009013039
6	34.998364351503945	34.604072764755166	15.666002120170786	14.731560763570094
7	28.92663653575821	30.59083172726024	21.54542550778399	18.93710622919756
8	27.033219095116046	33.518295345654195	20.133328498397525	19.315157060832234
9	26.57303864913951	13.872690996680866	20.024658172610295	39.52961218156933
10	16.962639319446783	26.673685038908374	33.100934942852604	23.262740698792246
11	35.5703784057365	21.70258663922069	21.617255165794578	21.109779789248233
12	21.512133888630856	25.726089056431416	29.0276686916818	23.734108363255928
13	29.66854200453352	19.90869686649868	25.648280023022522	24.774481105945274
14	22.896571464723227	21.275620659166343	25.228296410368777	30.599511465741653
15	25.684464888336127	27.732034484408103	22.625686470872342	23.957814156383428
16	25.27238948185433	26.515752375162215	24.186226296598623	24.025631846384833
17	23.55850760962317	26.63919754467558	25.597397467213735	24.20489737848752
18	23.366743254107252	25.08324833619057	27.317413021305093	24.23259538839708
19	24.040985339343056	25.61684008141209	27.020103045855254	23.322071533389604
20	24.51223727396032	24.80858283402334	26.933922886888734	23.745257005127606
21	24.868260858159957	25.411458180634227	26.061204115044728	23.659076846161085
22	24.606981441561828	25.155232300663055	26.28957767864445	23.948208579130668
23	23.79802981509457	25.409529349860584	26.62693018095519	24.165510654089662
24	24.11038467057885	25.802972251068958	26.37676082961326	23.709882248738932
25	24.386130317979326	25.242029685477142	26.40731350906782	23.964526487475716
26	24.03604753256252	26.060201123042432	26.355736574180515	23.548014770214532
27	24.51655785489329	25.25784609782104	25.96665283052058	24.25894321676509
28	23.598588713099538	25.54366024185995	26.86888271320138	23.988868331839132
29	23.31358467798556	25.953151015105057	26.905106155130458	23.828158151778922
30	23.199899392186847	26.516832520395457	26.860665894105644	23.422602193312052
31	23.98859829553082	26.134576837674235	26.109463461001358	23.76736140579359
32	23.190023778625775	25.729715258285875	26.502829208978785	24.577431754109565
33	23.250936254457528	25.47067328538517	26.915598994539092	24.362791465618205
34	23.959781563772545	25.38021112210116	26.658755888720354	24.001251425405943
35	23.924021041229143	25.41813193511105	27.18116041545472	23.476686608205092
36	23.267408469264467	25.95662291049762	26.666548365045884	24.109420255192028
37	24.085232717290502	24.96983308670017	26.75056823354592	24.19436596246341
38	23.255372565236915	25.004166274471075	27.57522054251066	24.16524061778135
39	23.506120565810935	24.920686478587665	27.335235417653585	24.237957537947814
40	22.980282720299478	24.912276776414565	28.084123253829503	24.023317249456454
41	22.327797846190407	25.035413333004147	28.50931470957209	24.127474111233358
42	23.112060438754995	24.82898986360852	28.19418233777376	23.86476735986273
43	22.369036248130964	24.475628065876514	28.25590492253044	24.89943076346208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	335.0
1	321.5
2	308.0
3	855.0
4	1402.0
5	1402.0
6	1954.5
7	2507.0
8	2426.5
9	2346.0
10	3501.5
11	4657.0
12	4657.0
13	7927.5
14	11198.0
15	15556.5
16	19915.0
17	20013.0
18	20111.0
19	20111.0
20	24346.5
21	28582.0
22	29140.0
23	29698.0
24	33842.5
25	37987.0
26	37987.0
27	42812.0
28	47637.0
29	57177.5
30	66718.0
31	73756.5
32	80795.0
33	80795.0
34	88948.5
35	97102.0
36	106035.0
37	114968.0
38	126983.5
39	138999.0
40	138999.0
41	145607.0
42	152215.0
43	150924.0
44	149633.0
45	155813.5
46	161994.0
47	161994.0
48	166735.0
49	171476.0
50	175035.5
51	178595.0
52	186156.0
53	193717.0
54	193717.0
55	183232.0
56	172747.0
57	165162.0
58	157577.0
59	146566.0
60	135555.0
61	135555.0
62	126342.0
63	117129.0
64	104211.0
65	91293.0
66	79819.0
67	68345.0
68	68345.0
69	58631.0
70	48917.0
71	42434.0
72	35951.0
73	28509.0
74	21067.0
75	21067.0
76	16510.5
77	11954.0
78	9797.0
79	7640.0
80	6128.5
81	4617.0
82	4617.0
83	3469.5
84	2322.0
85	2079.5
86	1837.0
87	1638.0
88	1439.0
89	1439.0
90	1125.5
91	812.0
92	457.0
93	102.0
94	69.5
95	37.0
96	37.0
97	22.0
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2592244.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.767922030807284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.76559293624292	47.272009777635446
2	9.40613307484527	10.491209918587373
3	2.467626656745122	4.128432329835109
4	1.0047135429112621	2.2412314609748565
5	0.5209251568022025	1.4525456764215647
6	0.3410576178615234	1.1412044784548583
7	0.23596455804218722	0.9211477152451417
8	0.16488911614448556	0.7356418698299518
9	0.11858506880327144	0.5951918583934883
>10	0.7521320590943156	8.068187972683255
>50	0.10038187985003812	3.9295755094751
>100	0.10955115532140158	12.631087345817463
>500	0.009542835957511852	3.5741563408491843
>1k	0.0026968884227745234	1.9925282400811963
>5k	2.0745295559804024E-4	0.8258495057160877
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8182	0.3156338677994819	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7190	0.27736586525033907	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6043	0.23311848730289278	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3943	0.15210759480974786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.857661547292616E-5	0.0	2.7003630831048313E-4	0.0
3	0.0	3.857661547292616E-5	0.0	7.32955693985597E-4	0.0
4	0.0	3.857661547292616E-5	0.0	0.0012344516951336372	0.0
5	0.0	7.715323094585232E-5	0.0	0.001427334772498268	0.0
6	0.0	7.715323094585232E-5	0.0	0.0015044880034441202	0.0
7	0.0	7.715323094585232E-5	0.0	0.0017745243117546034	0.0
8	0.0	7.715323094585232E-5	0.0	0.0019674073891192342	0.0
9	0.0	7.715323094585232E-5	0.0	0.0034333187770904283	0.0
10	0.0	7.715323094585232E-5	0.0	0.005362149550736736	0.0
11	0.0	7.715323094585232E-5	0.0	0.006172258475668185	0.0
12	0.0	7.715323094585232E-5	0.0	0.006750907707762078	0.0
13	0.0	7.715323094585232E-5	0.0	0.007368133555328896	0.0
14	0.0	1.1572984641877848E-4	0.0	0.007753899710058158	0.0
15	0.0	1.1572984641877848E-4	0.0	0.008409702173097902	3.857661547292616E-5
16	0.0	1.1572984641877848E-4	0.0	0.009875613561069098	3.857661547292616E-5
17	0.0	1.1572984641877848E-4	0.0	0.011418678179986144	3.857661547292616E-5
18	0.0	1.1572984641877848E-4	0.0	0.012498823413228076	3.857661547292616E-5
19	0.0	1.1572984641877848E-4	0.0	0.014041888032145122	3.857661547292616E-5
20	0.0	1.1572984641877848E-4	0.0	0.015623529266535095	3.857661547292616E-5
21	0.0	1.1572984641877848E-4	0.0	0.017706666502073108	3.857661547292616E-5
22	0.0	1.1572984641877848E-4	0.0	0.02141002158747402	3.857661547292616E-5
23	0.0	1.1572984641877848E-4	0.0	0.026887900984629533	3.857661547292616E-5
24	0.0	1.1572984641877848E-4	0.0	0.03630059516002351	3.857661547292616E-5
25	0.0	1.1572984641877848E-4	0.0	0.03946387762880346	3.857661547292616E-5
26	0.0	1.1572984641877848E-4	0.0	0.04509606348785068	3.857661547292616E-5
27	0.0	1.5430646189170465E-4	0.0	0.05582036258932415	3.857661547292616E-5
28	0.0	1.5430646189170465E-4	0.0	0.08506143711780219	3.857661547292616E-5
29	0.0	1.928830773646308E-4	0.0	0.12730283106065632	3.857661547292616E-5
30	0.0	1.928830773646308E-4	0.0	0.18821530689240673	3.857661547292616E-5
31	0.0	1.928830773646308E-4	0.0	0.4019683332278906	3.857661547292616E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	150	0.0	23.433332	15
GGTATCA	3455	0.0	19.115774	1
GTACCGA	95	7.0651426E-5	15.578948	6
TACCGCA	430	0.0	15.488372	28
AAGACGG	510	0.0	15.235294	5
ATTGCCG	455	0.0	15.043957	11
TAGTTCG	185	3.0559022E-10	15.0	7
ACGGACC	520	0.0	14.942307	8
TACGCAG	75	0.0041062552	14.799999	5
GACGGAC	525	0.0	14.799999	7
CGCAATA	380	0.0	14.605264	36
CGAGCCG	495	0.0	14.575758	15
GTATAAC	165	1.6478225E-8	14.575757	1
TTAACGG	140	6.002738E-7	14.535714	35
ATACCGC	460	0.0	14.478261	27
TAACGGC	155	1.2119017E-7	14.322581	36
TACCGTC	375	0.0	14.306667	7
CAAGACG	595	0.0	14.302521	4
TCTTATA	2090	0.0	14.0741625	37
CTTATAC	1370	0.0	14.043797	37
>>END_MODULE