FastQCFastQC Report
Fri 10 Feb 2017
ERR1630763.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630763.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601278
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT192513.2016804207039007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT180102.995286705982923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129422.1524153552932255No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87931.4623851196950497No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT29000.4823060215075223No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25290.42060411323880137No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24050.3999813730088246No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22530.37470188498498197No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT18820.31299997671626106RNA PCR Primer, Index 29 (95% over 23bp)
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA18110.3011917948103872No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA17920.2980318588074069No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14830.24664132065367433No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT13460.22385651894797415No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA13390.22269233199950772No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12610.20971996314516744No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA12080.20090540482106448No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA12060.2005727799786455No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.1970802191332462No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG11450.19042772228486657No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC11410.1897624726000286No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC11320.1882656608091432RNA PCR Primer, Index 29 (95% over 21bp)
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA9620.15999254920352982No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.1583294249914349No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA9450.15716523804296847No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8980.14934855424612242No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8970.14918224182491294No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.14868330456128445No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA8560.14236343255532383No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7740.1287258140161456No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7730.12855950159493612No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA7450.12390275380107038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7350.1222396295889755No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC7350.1222396295889755No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA7320.12174069232534701No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG7200.11974494327083314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC6910.11492188305575791No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA6630.11026513526189217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT6320.10510945020439795No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA6120.10178320178020817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT200.001841238437.04
CCCGTAT251.2329257E-436.9999961
AACAGAA303.597455E-430.8333327
CCGTATA405.935492E-527.752
AAGTAAT358.864456E-426.4285734
TACTGTG358.864456E-426.428577
CCGTCTT2400.024.66666637
GTACTAT601.3356348E-624.6666661
GCCGTCT2500.023.6836
GGTACTA400.001930122423.1255
TAAAGAA400.001930122423.1254
TCATTGC400.001930122423.12527
TGTGCAA400.001930122423.12520
GTAATCT400.001930122423.12536
GAAGTAA400.001930122423.12533
TATACTG400.001930122423.1255
TTCGCTA400.001930122423.12526
CAATCAT400.001930122423.12524
ATCATTG400.001930122423.12526
AAACTCT502.7000264E-422.19999937
Produced by FastQC (version 0.11.5)