##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630763.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 601278 Sequences flagged as poor quality 0 Sequence length 43 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84889186033748 33.0 31.0 34.0 30.0 34.0 2 31.89672830204997 33.0 31.0 34.0 30.0 34.0 3 31.886732925535277 33.0 31.0 34.0 30.0 34.0 4 35.57271511680121 37.0 35.0 37.0 33.0 37.0 5 35.615459072176264 37.0 35.0 37.0 33.0 37.0 6 35.73088820811671 37.0 35.0 37.0 35.0 37.0 7 35.65701223061546 37.0 35.0 37.0 33.0 37.0 8 35.743449785290665 37.0 35.0 37.0 33.0 37.0 9 37.30502196987084 39.0 37.0 39.0 34.0 39.0 10 37.23163162463952 39.0 37.0 39.0 34.0 39.0 11 37.40700640968071 39.0 37.0 39.0 34.0 39.0 12 37.36681036059859 39.0 37.0 39.0 34.0 39.0 13 37.44820033329009 39.0 37.0 39.0 35.0 39.0 14 38.4967136665569 40.0 38.0 41.0 34.0 41.0 15 38.43598801220068 40.0 38.0 41.0 34.0 41.0 16 38.3089901842409 40.0 38.0 41.0 34.0 41.0 17 38.484389916145275 40.0 38.0 41.0 34.0 41.0 18 38.59257614614205 40.0 38.0 41.0 34.0 41.0 19 38.68938494340388 40.0 38.0 41.0 34.0 41.0 20 38.70882187607064 40.0 38.0 41.0 35.0 41.0 21 38.64089988324868 40.0 38.0 41.0 34.0 41.0 22 38.51644663533341 40.0 38.0 41.0 34.0 41.0 23 38.44653221970536 40.0 38.0 41.0 34.0 41.0 24 38.334399395953284 40.0 38.0 41.0 34.0 41.0 25 38.21882889445481 40.0 37.0 41.0 34.0 41.0 26 37.84221441662592 40.0 37.0 41.0 33.0 41.0 27 37.57252385751682 40.0 35.0 41.0 33.0 41.0 28 37.376950428919734 39.0 35.0 41.0 33.0 41.0 29 37.123668253287164 39.0 35.0 41.0 32.0 41.0 30 36.8036548817685 39.0 35.0 41.0 32.0 41.0 31 36.45975405719152 38.0 35.0 40.0 31.0 41.0 32 36.0924048443482 38.0 35.0 40.0 31.0 41.0 33 35.829489853279185 38.0 35.0 40.0 30.0 41.0 34 35.544377143351326 37.0 35.0 40.0 30.0 41.0 35 35.24391546007005 37.0 35.0 40.0 28.0 41.0 36 34.91422603188542 37.0 35.0 40.0 25.0 41.0 37 34.3955774200952 36.0 34.0 40.0 22.0 41.0 38 33.97245866304771 36.0 33.0 40.0 21.0 41.0 39 33.51958661384584 35.0 33.0 40.0 18.0 41.0 40 33.00722461157734 35.0 33.0 40.0 15.0 41.0 41 32.45946134733018 35.0 33.0 40.0 10.0 41.0 42 31.91341941664255 35.0 31.0 40.0 8.0 41.0 43 30.97394549609332 35.0 29.0 39.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 13.0 11 10.0 12 11.0 13 14.0 14 3.0 15 4.0 16 8.0 17 24.0 18 43.0 19 93.0 20 215.0 21 450.0 22 808.0 23 1310.0 24 2112.0 25 3407.0 26 5000.0 27 7453.0 28 9917.0 29 13139.0 30 16200.0 31 19265.0 32 23663.0 33 28982.0 34 35784.0 35 43821.0 36 59477.0 37 102486.0 38 111390.0 39 116172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.03405745761528 20.14442570657832 13.837027132208396 16.984489703598 2 17.450829732669416 25.488210112460457 33.03097735157448 24.029982803295646 3 23.438076896211072 28.731468638466733 30.33405512924138 17.496399336080813 4 13.094109546665603 18.143354654585732 30.235930800727782 38.52660499802088 5 10.57830155102964 44.82402482711824 30.445817076294162 14.151856545557962 6 29.56386230662023 31.542481181749537 18.716799882916053 20.17685662871417 7 24.438446109786156 36.97673954476964 21.164752410698544 17.42006193474566 8 33.4939911322217 29.78206420324708 19.6200426425048 17.103902022026418 9 28.564657279993614 11.229913617328425 23.479488689092236 36.725940413585725 10 21.043676968058037 31.76750188764598 26.301477852174866 20.887343292121116 11 28.257145613177265 25.979330692292084 19.649978878322507 26.113544816208144 12 20.53625777094788 34.29944218813926 27.49975884698925 17.664541193923608 13 30.22063005797651 21.062969208918336 30.251065231057844 18.465335502047306 14 22.525354328613385 24.598272346568475 31.89722557618938 20.979147748628755 15 27.844025558892895 25.607289806046456 29.48153765812154 17.06714697693912 16 20.361130791414286 26.225805700524553 32.87530892532239 20.537754582738764 17 17.38280795239473 32.269931712119856 31.40377662246083 18.94348371302459 18 16.41736434727364 25.16107357994139 36.49426721084091 21.92729486194406 19 17.52034832473498 28.601578637502122 38.96932866328054 14.908744374482353 20 18.854506567677515 24.61989296132571 39.97452093707071 16.55107953392607 21 20.831462318594728 25.54342583630201 37.59525543924774 16.02985640585553 22 20.174860879659658 27.583580307278833 35.695634964192934 16.545923848868576 23 18.3233046943344 27.663243957038176 36.53085594350699 17.482595405120428 24 16.551911096032118 30.178054078146882 36.65126613646267 16.618768689358333 25 16.354165627214034 29.44361842608577 36.85616303939276 17.346052907307435 26 17.1228616380443 29.735164100466005 36.69284424176504 16.449130019724652 27 16.605962632925202 30.834156579818323 35.96888627224013 16.590994515016348 28 15.616403726728734 30.286988714039097 37.945509398314925 16.151098160917247 29 15.644011588649509 31.399951436773005 37.71001100988228 15.246025964695198 30 15.32419280266366 31.49009276906855 38.378088005880805 14.807626422386983 31 14.421116355496128 32.68937163841019 36.26209507083246 16.627416935261227 32 14.01797504648432 33.11296937523076 37.12791753564907 15.741138042635852 33 14.602396894614472 32.29587644982853 37.47218424755271 15.629542408004285 34 14.991069022981051 32.82940669706858 35.62212487401834 16.55739940593203 35 14.16516153925472 33.146398171893864 36.131539820183015 16.556900468668402 36 14.701352785234118 33.47087370567358 34.3526621629263 17.475111346166 37 14.346608390794275 33.134091052724365 35.1060574310053 17.41324312547607 38 15.035807064286402 31.22499076966062 35.71891870316227 18.02028346289071 39 14.912403247748962 32.259620342004865 35.27636135032381 17.551615059922366 40 14.112274189310103 32.08748698605304 35.65189479741484 18.148344027222016 41 13.976729566024368 32.37786847348481 34.61393897664641 19.03146298384441 42 14.118926686158481 32.079337677413776 35.2095037569976 18.592231879430145 43 14.002341678890629 31.950611863397633 34.54558457152931 19.50146188618243 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 67.0 1 167.0 2 267.0 3 1063.5 4 1860.0 5 1860.0 6 2970.5 7 4081.0 8 4430.5 9 4780.0 10 7064.5 11 9349.0 12 9349.0 13 16889.5 14 24430.0 15 34242.0 16 44054.0 17 39344.0 18 34634.0 19 34634.0 20 37660.0 21 40686.0 22 29588.5 23 18491.0 24 16449.5 25 14408.0 26 14408.0 27 14776.5 28 15145.0 29 15201.0 30 15257.0 31 15568.0 32 15879.0 33 15879.0 34 17128.5 35 18378.0 36 18976.5 37 19575.0 38 22082.0 39 24589.0 40 24589.0 41 26527.0 42 28465.0 43 31003.5 44 33542.0 45 33708.5 46 33875.0 47 33875.0 48 36758.5 49 39642.0 50 37944.5 51 36247.0 52 29076.0 53 21905.0 54 21905.0 55 21483.5 56 21062.0 57 18866.0 58 16670.0 59 14832.0 60 12994.0 61 12994.0 62 12599.5 63 12205.0 64 11322.5 65 10440.0 66 9468.0 67 8496.0 68 8496.0 69 7776.0 70 7056.0 71 6066.0 72 5076.0 73 4233.0 74 3390.0 75 3390.0 76 2733.0 77 2076.0 78 1620.0 79 1164.0 80 879.5 81 595.0 82 595.0 83 430.0 84 265.0 85 191.0 86 117.0 87 81.0 88 45.0 89 45.0 90 29.5 91 14.0 92 10.5 93 7.0 94 3.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 601278.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.92832621960408 #Duplication Level Percentage of deduplicated Percentage of total 1 77.67993410684818 27.13230079201343 2 10.916145080051013 7.625653528330957 3 3.895612304700268 4.08201652211024 4 1.8607028420041103 2.59964943453056 5 1.1131814748718807 1.9440782847972526 6 0.7249935657573312 1.519368706313163 7 0.5381453821665912 1.3157562223321746 8 0.3842260041859415 1.073629697300922 9 0.29833432249161934 0.9378286684642664 >10 2.1213792556505724 14.708292020444153 >50 0.24108721932591545 5.832053648285123 >100 0.20425444970655462 13.676905964440275 >500 0.011958691434809989 3.014269176321077 >1k 0.008131910175670791 4.68121466560412 >5k 4.783476573923996E-4 1.469124154281427 >10k+ 0.0014350429721771986 8.387858514430853 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 19251 3.2016804207039007 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 18010 2.995286705982923 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 12942 2.1524153552932255 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8793 1.4623851196950497 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT 2900 0.4823060215075223 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2529 0.42060411323880137 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2405 0.3999813730088246 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2253 0.37470188498498197 No Hit TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT 1882 0.31299997671626106 RNA PCR Primer, Index 29 (95% over 23bp) ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 1811 0.3011917948103872 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 1792 0.2980318588074069 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1483 0.24664132065367433 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 1346 0.22385651894797415 No Hit GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA 1339 0.22269233199950772 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1261 0.20971996314516744 No Hit GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 1208 0.20090540482106448 No Hit TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA 1206 0.2005727799786455 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1185 0.1970802191332462 No Hit ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG 1145 0.19042772228486657 No Hit ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC 1141 0.1897624726000286 No Hit CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC 1132 0.1882656608091432 RNA PCR Primer, Index 29 (95% over 21bp) ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 962 0.15999254920352982 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.1583294249914349 No Hit CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA 945 0.15716523804296847 No Hit GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 898 0.14934855424612242 No Hit GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG 897 0.14918224182491294 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 894 0.14868330456128445 No Hit GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA 856 0.14236343255532383 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 774 0.1287258140161456 No Hit GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 773 0.12855950159493612 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA 745 0.12390275380107038 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 735 0.1222396295889755 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 735 0.1222396295889755 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA 732 0.12174069232534701 No Hit GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG 720 0.11974494327083314 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 691 0.11492188305575791 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA 663 0.11026513526189217 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 632 0.10510945020439795 No Hit GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA 612 0.10178320178020817 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.6631242120949044E-4 0.0 2 0.0 0.0 0.0 4.989372636284713E-4 0.0 3 0.0 0.0 0.0 9.978745272569427E-4 0.0 4 0.0 0.0 0.0 0.0011641869484664332 0.0 5 0.0 0.0 0.0 0.0011641869484664332 0.0 6 0.0 0.0 0.0 0.0011641869484664332 0.0 7 0.0 0.0 0.0 0.001496811790885414 0.0 8 0.0 0.0 0.0 0.001829436633304395 0.0 9 0.0 0.0 0.0 0.002494686318142357 0.0 10 0.0 1.6631242120949044E-4 0.0 0.00365887326660879 0.0 11 0.0 1.6631242120949044E-4 0.0 0.004823060215075223 0.0 12 0.0 3.326248424189809E-4 0.0 0.007151434112008089 0.0 13 0.0 3.326248424189809E-4 0.0 0.008814558324102995 0.0 14 0.0 3.326248424189809E-4 0.0 0.011808181905873821 0.0 15 0.0 3.326248424189809E-4 0.0 0.015965992436111083 0.0 16 0.0 3.326248424189809E-4 0.0 0.030435173081336753 0.0 17 0.0 4.989372636284713E-4 0.0 0.047232727623495285 0.0 18 0.0 6.652496848379618E-4 0.0 0.05338628720824644 0.0 19 0.0 6.652496848379618E-4 0.0 0.06519446911412026 0.0 20 0.0 6.652496848379618E-4 0.0 0.07533952680789917 0.0 21 0.0 6.652496848379618E-4 0.0 0.0976253912499709 0.0 22 0.0 6.652496848379618E-4 0.0 0.1476854300340275 0.0 23 0.0 6.652496848379618E-4 0.0 0.21005258798758644 0.0 24 0.0 8.315621060474523E-4 0.0 0.3134989139798895 0.0 25 0.0 8.315621060474523E-4 0.0 0.3384457771613131 0.0 26 0.0 8.315621060474523E-4 0.0 0.3781944458303813 0.0 27 0.0 8.315621060474523E-4 0.0 0.4237640492417817 0.0 28 0.0 8.315621060474523E-4 0.0 0.48496702024687416 0.0 29 0.0 8.315621060474523E-4 0.0 0.5722810413818566 0.0 30 0.0 8.315621060474523E-4 0.0 0.7435828352276318 0.0 31 0.0 8.315621060474523E-4 0.0 1.2475094714923878 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGGT 20 0.0018412384 37.0 4 CCCGTAT 25 1.2329257E-4 36.999996 1 AACAGAA 30 3.597455E-4 30.833332 7 CCGTATA 40 5.935492E-5 27.75 2 AAGTAAT 35 8.864456E-4 26.42857 34 TACTGTG 35 8.864456E-4 26.42857 7 CCGTCTT 240 0.0 24.666666 37 GTACTAT 60 1.3356348E-6 24.666666 1 GCCGTCT 250 0.0 23.68 36 GGTACTA 40 0.0019301224 23.125 5 TAAAGAA 40 0.0019301224 23.125 4 TCATTGC 40 0.0019301224 23.125 27 TGTGCAA 40 0.0019301224 23.125 20 GTAATCT 40 0.0019301224 23.125 36 GAAGTAA 40 0.0019301224 23.125 33 TATACTG 40 0.0019301224 23.125 5 TTCGCTA 40 0.0019301224 23.125 26 CAATCAT 40 0.0019301224 23.125 24 ATCATTG 40 0.0019301224 23.125 26 AAACTCT 50 2.7000264E-4 22.199999 37 >>END_MODULE