FastQCFastQC Report
Fri 10 Feb 2017
ERR1630762.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630762.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2462727
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88590.35972318490843685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73770.29954599109036445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68910.2798117696358549No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47320.19214472412086278No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG30370.12331858139371518No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT25410.1031783059998124No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG24930.10122924709072503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39450.017.8669191
TCTAGCG2850.017.52631628
CTAGCGG3000.016.6529
CGAATTA2450.016.61224415
TACCGTG803.3845284E-416.18757
GTATAGA1954.1836756E-1115.1794871
CGCAATA3450.015.01449236
CTAGCAC4600.014.478263
TAGAACA10750.013.9395354
CCGCTTA800.006301315513.87500125
GTACTAG800.006301315513.8750011
AAGACGG4400.013.8755
TCTAATA3500.013.7428572
TAGCACT5950.013.6806734
CTTATAC12950.013.42857237
ATAACGC3500.013.2142853
GTAGAAC9850.013.1472073
GTACCGT850.00940872313.0588236
CGAACGA2703.6379788E-1213.01851816
TAAACCG1857.393464E-813.05
Produced by FastQC (version 0.11.5)