FastQCFastQC Report
Fri 10 Feb 2017
ERR1630761.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630761.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127356
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135031.1977582946292031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT110880.9835402481558622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92470.8202377953370541No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70630.6265101707002934No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG21620.19177615589041971No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20760.1841476871547231No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19140.16977778093166665No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.16365726531814262No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT17610.15620620283211337No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG17420.15452084346027342No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG15760.13979612473788225No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG15660.13890909348954544No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT15420.13678021849353708TruSeq Adapter, Index 6 (95% over 21bp)
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14240.12631324976316266No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.1252488122651585No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13750.12196679664631227No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG13690.12143457789731016No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG12870.11416092166094828No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC12820.11371740603677985No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG12710.11274167166360936No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12670.11238685916427464No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12390.10990317166893156No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT12320.10928224979509578No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT12200.1082178122970916No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG12040.10679856229975268No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11830.10493579667824539No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC11560.10254081230773597No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT11490.10191989043390022No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT11480.10183118730906654No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC11310.10032323418689394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2500.023.6836
CCGTCTT2550.023.21568537
CCAGTAC502.702058E-422.1999993
GCAGCGT705.1017796E-621.1428571
TTGGAGC450.00382576320.5555553
CCAGGAC450.00382576320.5555553
GTTCGCA450.00382576320.55555511
GTGTACA450.00382576320.5555551
AGACATA909.477844E-820.55555514
AGCCTTA450.00382576320.5555556
ATAGATG1001.2896635E-820.3499986
AGCGTCA851.2452638E-619.5882363
TACCCTT1103.8513463E-818.55
TACATGA701.219171E-418.52
TCATGGA609.2366664E-418.52
AGACTAA500.007034801418.49999816
ACCTCGC500.007034801418.49999820
GAGACAT1002.8748036E-718.49999813
CATCAGA500.007034801418.49999816
GCTATAT1653.6379788E-1217.93939419
Produced by FastQC (version 0.11.5)