##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630761.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1127356
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.907890675172705	33.0	31.0	34.0	30.0	34.0
2	32.05219824083963	33.0	31.0	34.0	30.0	34.0
3	32.11478450462853	34.0	31.0	34.0	30.0	34.0
4	35.720103498806054	37.0	35.0	37.0	33.0	37.0
5	35.72815508144721	37.0	35.0	37.0	35.0	37.0
6	35.800657467561265	37.0	35.0	37.0	35.0	37.0
7	35.773319164487525	37.0	35.0	37.0	35.0	37.0
8	35.78711782258665	37.0	35.0	37.0	35.0	37.0
9	37.464625193816325	39.0	37.0	39.0	35.0	39.0
10	37.36406423525488	39.0	37.0	39.0	34.0	39.0
11	37.49863752000255	39.0	37.0	39.0	35.0	39.0
12	37.44259665979513	39.0	37.0	39.0	34.0	39.0
13	37.49723689766143	39.0	37.0	39.0	35.0	39.0
14	38.701484712903465	40.0	38.0	41.0	35.0	41.0
15	38.65366751939937	40.0	38.0	41.0	34.0	41.0
16	38.60017687403092	40.0	38.0	41.0	34.0	41.0
17	38.64146196942226	40.0	38.0	41.0	34.0	41.0
18	38.65799268376627	40.0	38.0	41.0	34.0	41.0
19	38.71916413271407	40.0	38.0	41.0	34.0	41.0
20	38.67743286060481	40.0	38.0	41.0	34.0	41.0
21	38.60931152182629	40.0	38.0	41.0	34.0	41.0
22	38.53407619243611	40.0	38.0	41.0	34.0	41.0
23	38.498566557502684	40.0	38.0	41.0	34.0	41.0
24	38.44433435401062	40.0	38.0	41.0	34.0	41.0
25	38.361079375104225	40.0	38.0	41.0	34.0	41.0
26	38.10461469136635	40.0	37.0	41.0	33.0	41.0
27	37.94567465822686	40.0	37.0	41.0	33.0	41.0
28	37.82756733454206	40.0	37.0	41.0	33.0	41.0
29	37.70032625009314	40.0	37.0	41.0	33.0	41.0
30	37.544362206791824	40.0	36.0	41.0	33.0	41.0
31	37.42227743498948	39.0	36.0	41.0	33.0	41.0
32	37.24906950422049	39.0	35.0	41.0	32.0	41.0
33	37.143286592700086	39.0	35.0	41.0	32.0	41.0
34	37.01528177434635	39.0	35.0	41.0	31.0	41.0
35	36.867563573529566	39.0	35.0	41.0	31.0	41.0
36	36.760296658730695	39.0	35.0	41.0	31.0	41.0
37	36.48533737346499	39.0	35.0	41.0	30.0	41.0
38	36.293995862886256	39.0	35.0	41.0	30.0	41.0
39	36.121207497897736	39.0	35.0	41.0	30.0	41.0
40	35.88874587973985	39.0	35.0	41.0	28.0	41.0
41	35.66399078906752	39.0	35.0	41.0	26.0	41.0
42	35.4319451885651	39.0	35.0	41.0	25.0	41.0
43	34.58436376796682	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	12.0
11	4.0
12	3.0
13	2.0
14	1.0
15	0.0
16	6.0
17	11.0
18	54.0
19	101.0
20	251.0
21	420.0
22	803.0
23	1392.0
24	2349.0
25	3780.0
26	5676.0
27	8325.0
28	11703.0
29	16010.0
30	20736.0
31	26403.0
32	32923.0
33	42155.0
34	54872.0
35	70734.0
36	97688.0
37	155084.0
38	246638.0
39	329218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2187738389648	21.184257679029518	12.753380476087411	21.84358800591827
2	19.288139682584738	22.032348255564347	34.3650098105656	24.314502251285308
3	21.246882085162095	26.475931294107628	29.000067414374875	23.2771192063554
4	13.562352974570588	17.03570123368306	35.10896291854569	34.29298287320066
5	12.264803664503493	40.405870017988995	33.775577546045795	13.553748771461722
6	31.123176707269046	35.456856574143394	16.766487249812837	16.653479468774727
7	27.139341964738733	32.41700048609312	22.77763191041694	17.6660256387512
8	28.13893747848949	32.157543845954606	20.122481274770347	19.581037400785554
9	27.244721277041144	13.207451772111028	22.375008426796857	37.17281852405097
10	19.852735072151123	27.725935729263874	31.50761605029822	20.91371314828679
11	32.21484606459716	24.070923470492016	21.161372272822426	22.55285819208839
12	21.71106553741675	29.91140331891612	27.207820777110335	21.169710366556792
13	28.46430054037944	21.16598483531378	28.018123822466016	22.351590801840768
14	22.49280617657599	22.77745450416727	27.709082135545472	27.020657183711265
15	26.194387575885525	27.23966519892563	25.999240701251424	20.566706523937427
16	23.581991846408766	26.08874215420861	28.046065306788627	22.283200692594
17	21.877738708979237	28.320157962524707	27.399508229875924	22.402595098620136
18	20.36251193057029	25.589520967644646	30.752663754838753	23.295303346946305
19	21.67629391248195	26.032060857439888	31.47461848786009	20.817026742218076
20	21.977086208792965	24.914667593909996	32.39828412675322	20.70996207054382
21	22.13728405224259	26.59860771575261	30.70893311429575	20.555175117709044
22	23.222300675208185	26.237585997679524	29.776840678543426	20.763272648568865
23	21.707694818673072	26.348021388097457	30.852366067151817	21.091917726077654
24	20.804963117240693	28.106206025425863	29.129662679756883	21.95916817757656
25	21.3669861161869	26.676666465606253	31.06720503549899	20.88914238270786
26	20.973321648175023	28.240591259548893	30.380465443036623	20.405621649239457
27	21.473163756612816	27.51624154215705	29.72104641302304	21.289548288207097
28	20.33643321186919	27.164711058441167	31.813375721599918	20.685480008089723
29	21.28795163196009	27.540013979612475	31.090800066704748	20.081234321722686
30	19.660515400636534	28.028856900570894	31.54017009711218	20.77045760168039
31	20.871046945241787	28.49188721220271	30.0177583655917	20.6193074769638
32	19.58795624452258	27.706687151174965	30.776968411043182	21.92838819325927
33	19.17016452655594	27.91363154141194	31.079800879225374	21.836403052806745
34	21.074886726109586	27.146349511600594	31.142691394732452	20.636072367557365
35	19.878991197101893	28.564712477691163	30.582885973907086	20.973410351299858
36	20.623742633205485	28.26436369700432	29.326494913762822	21.785398756027377
37	20.068194962372136	26.698753543689836	30.933618129499468	22.299433364438563
38	20.042737165544867	26.702124262433518	30.70476406742857	22.550374504593048
39	19.754540712960235	27.522095948396068	31.138522347865273	21.584840990778424
40	19.28530118259006	27.6393614794262	31.052569019901433	22.02276831808231
41	19.567554525810834	26.824711980953666	30.845181114040287	22.762552379195213
42	18.810739464729863	28.07932897860126	31.021168113710313	22.088763442958566
43	19.221878448333978	26.147197513474	30.950294316968197	23.68062972122382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1360.0
1	927.5
2	495.0
3	1095.0
4	1695.0
5	1695.0
6	2612.5
7	3530.0
8	3624.5
9	3719.0
10	5465.5
11	7212.0
12	7212.0
13	12817.5
14	18423.0
15	23646.0
16	28869.0
17	26433.0
18	23997.0
19	23997.0
20	25674.0
21	27351.0
22	20091.5
23	12832.0
24	11804.5
25	10777.0
26	10777.0
27	12277.0
28	13777.0
29	17075.0
30	20373.0
31	24652.5
32	28932.0
33	28932.0
34	37326.5
35	45721.0
36	50361.0
37	55001.0
38	64515.0
39	74029.0
40	74029.0
41	78117.0
42	82205.0
43	89192.5
44	96180.0
45	92736.5
46	89293.0
47	89293.0
48	90415.5
49	91538.0
50	88844.5
51	86151.0
52	77863.5
53	69576.0
54	69576.0
55	65322.5
56	61069.0
57	51425.0
58	41781.0
59	38779.0
60	35777.0
61	35777.0
62	32564.5
63	29352.0
64	25979.5
65	22607.0
66	19955.0
67	17303.0
68	17303.0
69	13694.0
70	10085.0
71	8587.0
72	7089.0
73	5357.0
74	3625.0
75	3625.0
76	2928.5
77	2232.0
78	1747.5
79	1263.0
80	963.5
81	664.0
82	664.0
83	596.0
84	528.0
85	483.5
86	439.0
87	373.0
88	307.0
89	307.0
90	232.0
91	157.0
92	97.5
93	38.0
94	20.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1127356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.116854782562804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.27348270783979	19.157499889331273
2	9.978147117151824	5.0123934428110015
3	3.648287837059263	2.74900547425223
4	1.859126379341107	1.867816291693694
5	1.1986579624852132	1.5053258988851859
6	0.8346870046166281	1.2578827370308907
7	0.6520665374080038	1.1464502370051772
8	0.5156404247448252	1.0361012534667824
9	0.44118186132384424	0.9972990669214582
>10	3.5234330849669133	18.384297076289517
>50	0.5587808882354457	9.879423586157273
>100	0.46428900319071	22.463203652303363
>500	0.03800988778990501	6.608141958741289
>1k	0.012788373462024116	4.285839069280717
>5k	7.10465192334673E-4	1.455231294263943
>10k+	7.10465192334673E-4	2.1940890715661943
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13503	1.1977582946292031	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11088	0.9835402481558622	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9247	0.8202377953370541	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7063	0.6265101707002934	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	2162	0.19177615589041971	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2076	0.1841476871547231	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1914	0.16977778093166665	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1845	0.16365726531814262	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	1761	0.15620620283211337	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1742	0.15452084346027342	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1576	0.13979612473788225	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	1566	0.13890909348954544	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	1542	0.13678021849353708	TruSeq Adapter, Index 6 (95% over 21bp)
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1424	0.12631324976316266	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1412	0.1252488122651585	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1375	0.12196679664631227	No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG	1369	0.12143457789731016	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1287	0.11416092166094828	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1282	0.11371740603677985	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	1271	0.11274167166360936	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1267	0.11238685916427464	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1239	0.10990317166893156	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	1232	0.10928224979509578	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	1220	0.1082178122970916	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	1204	0.10679856229975268	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1183	0.10493579667824539	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1156	0.10254081230773597	No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT	1149	0.10191989043390022	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	1148	0.10183118730906654	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	1131	0.10032323418689394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.870312483368163E-5	0.0
2	0.0	0.0	0.0	7.096249986694531E-4	0.0
3	0.0	0.0	0.0	0.0010644374980041797	0.0
4	0.0	0.0	0.0	0.0015966562470062695	0.0
5	0.0	0.0	0.0	0.0015966562470062695	0.0
6	0.0	0.0	0.0	0.0015966562470062695	0.0
7	0.0	0.0	0.0	0.0016853593718399512	0.0
8	0.0	0.0	0.0	0.002040171871174678	0.0
9	0.0	0.0	0.0	0.003193312494012539	0.0
10	0.0	0.0	0.0	0.004878671865852491	0.0
11	0.0	0.0	0.0	0.006031812488690351	0.0
12	0.0	0.0	0.0	0.007805874985363985	0.0
13	0.0	0.0	0.0	0.00860420310886712	0.0
14	0.0	0.0	0.0	0.010378265605540752	0.0
15	0.0	0.0	0.0	0.012861953100883838	0.0
16	0.0	0.0	0.0	0.020667828086247824	0.0
17	0.0	0.0	0.0	0.030957390566954894	0.0
18	0.0	8.870312483368163E-5	0.0	0.03548124993347266	0.0
19	0.0	8.870312483368163E-5	0.0	0.04310971866916928	0.0
20	0.0	8.870312483368163E-5	0.0	0.047367468661186	0.0
21	0.0	8.870312483368163E-5	0.0	0.058898874889564606	0.0
22	0.0	8.870312483368163E-5	0.0	0.07983281235031348	0.0
23	0.0	8.870312483368163E-5	0.0	0.10857262479642633	0.0
24	0.0	8.870312483368163E-5	0.0	0.15469824970994078	0.0
25	0.0	8.870312483368163E-5	0.0	0.16676187468732148	0.0
26	0.0	8.870312483368163E-5	0.0	0.19000209339374607	0.0
27	0.0	8.870312483368163E-5	0.0	0.21998374958753047	0.0
28	0.0	8.870312483368163E-5	0.0	0.27178637449040055	0.0
29	0.0	8.870312483368163E-5	0.0	0.34940160871987197	0.0
30	0.0	8.870312483368163E-5	0.0	0.48352073346839863	0.0
31	0.0	8.870312483368163E-5	0.0	0.8688471077459117	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	250	0.0	23.68	36
CCGTCTT	255	0.0	23.215685	37
CCAGTAC	50	2.702058E-4	22.199999	3
GCAGCGT	70	5.1017796E-6	21.142857	1
TTGGAGC	45	0.003825763	20.555555	3
CCAGGAC	45	0.003825763	20.555555	3
GTTCGCA	45	0.003825763	20.555555	11
GTGTACA	45	0.003825763	20.555555	1
AGACATA	90	9.477844E-8	20.555555	14
AGCCTTA	45	0.003825763	20.555555	6
ATAGATG	100	1.2896635E-8	20.349998	6
AGCGTCA	85	1.2452638E-6	19.588236	3
TACCCTT	110	3.8513463E-8	18.5	5
TACATGA	70	1.219171E-4	18.5	2
TCATGGA	60	9.2366664E-4	18.5	2
AGACTAA	50	0.0070348014	18.499998	16
ACCTCGC	50	0.0070348014	18.499998	20
GAGACAT	100	2.8748036E-7	18.499998	13
CATCAGA	50	0.0070348014	18.499998	16
GCTATAT	165	3.6379788E-12	17.939394	19
>>END_MODULE