FastQCFastQC Report
Fri 10 Feb 2017
ERR1630760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964003
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71610.36461247767951477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63050.32102802286961885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45520.23177154006383902No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG35430.1803968731208659No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT33150.16878792955000577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31310.15941930842264498No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG29120.14826861262431879No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA27880.14195497664718434No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC27820.14164947813216172No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA21000.1069244802579222No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC20230.1030039159817984No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC19790.10076359353829907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG502.703047E-422.230
TCTAGCG2950.018.1864428
GGTATCA31350.018.1754381
CGGTGAT3100.017.90322514
CGCAATA3000.017.88333336
CTAGCGG3050.017.59016429
CGCCTTA1900.017.52631625
GTCCTAT2950.016.9322011
AGTCGGT3350.016.56716311
TAGGGGT904.4485394E-516.4444454
TCGGTGA3500.016.38571513
TCTTATA22800.016.2280737
TAGAACA10900.016.1238544
CAGTCGG3450.016.08695610
GTGTAGG2200.015.9772731
CTTATAC14850.015.94612837
TATGCGG700.002593370415.8571435
CCGTAGT700.002593370415.85714327
TCGCCAT7700.015.85714213
TACTCCG1302.591496E-715.6538465
Produced by FastQC (version 0.11.5)