FastQCFastQC Report
Fri 10 Feb 2017
ERR1630759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377543
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55980.2354531547904707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46760.19667362483033954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44040.18523324288982365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30990.13034464571198082No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC25240.10616001477155197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT250.005497509629.66
GGTATCA26250.020.4380951
CGTCTAT555.145316E-420.1818181
AAGACGG5500.019.1727285
CAAGACG5750.018.0173914
CGCAAGA6200.017.0080642
GCGCAAG6350.016.0236231
ACGGACC6050.015.9008268
ACGCTTC2900.015.31034531
AGTACCG855.367144E-415.2352955
GACGGAC6450.014.9147297
TTAGAAC1508.112147E-814.83
TACCGTG750.00410612214.87
GAGCGAA6600.014.57575716
AACGATT2059.276846E-1114.43902522
CGATTAA1803.3360266E-914.38888824
GCTTTAT6950.014.3741011
GAGTACG5950.014.3025211
CGGACCA6900.014.2101449
CTAGCAC4300.014.1976763
Produced by FastQC (version 0.11.5)