##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630759.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2377543
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.873988819550267	33.0	31.0	34.0	30.0	34.0
2	32.04520969757434	33.0	31.0	34.0	30.0	34.0
3	32.14806419904919	34.0	31.0	34.0	30.0	34.0
4	35.76039381832421	37.0	35.0	37.0	33.0	37.0
5	35.72263803430685	37.0	35.0	37.0	35.0	37.0
6	35.75428625265663	37.0	35.0	37.0	35.0	37.0
7	35.7311081229656	37.0	35.0	37.0	33.0	37.0
8	35.71401316401007	37.0	35.0	37.0	33.0	37.0
9	37.419014503628325	39.0	37.0	39.0	34.0	39.0
10	37.30628846670702	39.0	37.0	39.0	34.0	39.0
11	37.412688645378864	39.0	37.0	39.0	34.0	39.0
12	37.34007040040916	39.0	37.0	39.0	34.0	39.0
13	37.39354577393553	39.0	37.0	39.0	34.0	39.0
14	38.64512145521658	40.0	38.0	41.0	34.0	41.0
15	38.62122914285882	40.0	38.0	41.0	34.0	41.0
16	38.597664479675025	40.0	38.0	41.0	34.0	41.0
17	38.58620096460926	40.0	38.0	41.0	34.0	41.0
18	38.55327411533671	40.0	38.0	41.0	34.0	41.0
19	38.573608973633704	40.0	38.0	41.0	34.0	41.0
20	38.55657374020154	40.0	38.0	41.0	34.0	41.0
21	38.48064451410553	40.0	38.0	41.0	34.0	41.0
22	38.429447963717166	40.0	38.0	41.0	34.0	41.0
23	38.406958359953954	40.0	38.0	41.0	34.0	41.0
24	38.3576675584837	40.0	38.0	41.0	34.0	41.0
25	38.30983077908581	40.0	38.0	41.0	34.0	41.0
26	38.11844033945969	40.0	38.0	41.0	33.0	41.0
27	38.005144386452734	40.0	37.0	41.0	33.0	41.0
28	37.90531906257847	40.0	37.0	41.0	33.0	41.0
29	37.81674442901769	40.0	37.0	41.0	33.0	41.0
30	37.74814461820459	40.0	37.0	41.0	33.0	41.0
31	37.70773357201111	40.0	37.0	41.0	33.0	41.0
32	37.62335654917703	40.0	37.0	41.0	32.0	41.0
33	37.591159444855464	40.0	37.0	41.0	32.0	41.0
34	37.53065622787895	40.0	37.0	41.0	32.0	41.0
35	37.47952739445722	40.0	37.0	41.0	32.0	41.0
36	37.442194315728464	40.0	37.0	41.0	32.0	41.0
37	37.265349564655615	40.0	36.0	41.0	31.0	41.0
38	37.15583903214369	39.0	36.0	41.0	31.0	41.0
39	37.10698818065541	39.0	36.0	41.0	31.0	41.0
40	37.01288220654684	39.0	36.0	41.0	31.0	41.0
41	36.902121223464725	39.0	35.0	41.0	31.0	41.0
42	36.8247573230011	39.0	35.0	41.0	30.0	41.0
43	35.950746632132415	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	2.0
11	2.0
12	3.0
13	3.0
14	0.0
15	5.0
16	18.0
17	48.0
18	94.0
19	192.0
20	376.0
21	739.0
22	1342.0
23	2272.0
24	3935.0
25	6123.0
26	9093.0
27	13443.0
28	19675.0
29	27462.0
30	36585.0
31	48343.0
32	62660.0
33	81172.0
34	106685.0
35	142556.0
36	201718.0
37	309984.0
38	582464.0
39	720543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54945420545496	19.15776076394833	12.15040064469917	25.142384385897543
2	19.08621631659238	21.009924951935673	34.066050540410835	25.837808191061107
3	20.97501496292601	23.426411215275603	28.930664976406316	26.66790884539207
4	15.013482405996442	15.10109386034238	33.973307738282756	35.91211599537842
5	14.623836456375342	36.86192005780758	34.106891021529364	14.407352464287712
6	35.657062774469274	34.09767983165815	15.296758039707377	14.948499354165204
7	29.108117077167478	30.138382355229748	21.446341874784178	19.307158692818593
8	27.558786528782026	32.67924071194506	20.12577690498132	19.636195854291593
9	26.602126649234105	14.085381421072091	19.727424488221665	39.58506744147214
10	17.42298667153444	26.600486300352927	32.50679377828287	23.469733249829762
11	35.90605932258639	21.76301332930677	21.393093626487513	20.937833721619338
12	22.32636801942173	25.510032836419782	28.00491936423442	24.158679779924064
13	29.839081774756544	19.66891871145969	25.594489773686536	24.897509740097234
14	23.05872070452564	21.03861002724241	24.617893346198155	31.2847759220338
15	25.868049494793578	27.91259716438357	22.283172165550738	23.936181175272118
16	25.850510379833295	26.22522494861292	23.64840509719488	24.275859574358908
17	24.428285839625193	26.2547512284741	24.714000966544035	24.602961965356673
18	23.69181125220448	24.78285355932574	26.866811662291703	24.658523526178076
19	24.965268766958157	25.131448726689698	26.478091037680496	23.42519146867165
20	25.343474334638742	24.318971307774454	26.06211538550512	24.275438972081684
21	25.111806600343296	25.22970141864942	25.556341147142238	24.102150833865043
22	25.145833324570788	24.815954958543337	25.8245171591008	24.213694557785075
23	24.209320294101936	25.090818546709777	25.91591403394176	24.783947125246524
24	24.723674818920205	25.48576408502391	25.90030968945672	23.890251406599166
25	24.881400672879522	24.97103101815614	25.68163856552752	24.465929743436817
26	24.704074752801528	25.71852538524014	25.702500438477877	23.874899423480457
27	25.228481672045465	24.448348568248818	25.556088785775906	24.767080973929808
28	24.03674717975658	25.174602520332968	26.195488367613116	24.593161932297335
29	24.226228505646375	25.699430041854132	26.1294117498611	23.944929702638397
30	23.92007210805441	25.94632357858512	25.901108833783447	24.232495479577025
31	24.180088435834808	25.828639061417608	25.86985808458564	24.121414418161944
32	23.838138784451008	25.176579351035922	25.643195517389174	25.3420863471239
33	23.43246788806764	25.45228414375681	26.193175055088386	24.922072913087163
34	24.909875447047643	24.814609031256214	25.900267629228996	24.37524789246714
35	24.567967855891563	24.865165424978645	26.283015701503608	24.28385101762618
36	23.648699518788934	25.748219906012217	26.26253237060276	24.34054820459609
37	24.778353114959433	24.255544484368947	26.407303674423556	24.558798726248064
38	24.11527362491446	24.602078700574502	26.67325049431283	24.609397180198215
39	24.205703114517803	24.46921044119917	26.651421236124857	24.67366520815817
40	23.578332757809218	24.277121381190582	27.339400380981544	24.80514548001866
41	22.638496969350292	24.932209427968285	27.913480429165737	24.515813173515685
42	23.85222896073804	24.28704759493309	27.76572284917665	24.09500059515222
43	23.315624575454578	24.043602996875347	27.551089507108813	25.08968292056127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	461.0
1	398.5
2	336.0
3	814.0
4	1292.0
5	1292.0
6	1657.0
7	2022.0
8	1904.5
9	1787.0
10	2742.5
11	3698.0
12	3698.0
13	6130.0
14	8562.0
15	11557.0
16	14552.0
17	15228.0
18	15904.0
19	15904.0
20	19144.0
21	22384.0
22	23903.5
23	25423.0
24	28721.5
25	32020.0
26	32020.0
27	35543.0
28	39066.0
29	48213.5
30	57361.0
31	61971.5
32	66582.0
33	66582.0
34	74004.5
35	81427.0
36	88928.5
37	96430.0
38	109403.5
39	122377.0
40	122377.0
41	129223.5
42	136070.0
43	133345.0
44	130620.0
45	138243.5
46	145867.0
47	145867.0
48	154800.5
49	163734.0
50	167070.0
51	170406.0
52	176983.0
53	183560.0
54	183560.0
55	168233.5
56	152907.0
57	148350.5
58	143794.0
59	138892.5
60	133991.0
61	133991.0
62	125058.0
63	116125.0
64	105522.0
65	94919.0
66	83608.5
67	72298.0
68	72298.0
69	64449.0
70	56600.0
71	48335.5
72	40071.0
73	29373.0
74	18675.0
75	18675.0
76	14171.0
77	9667.0
78	8002.5
79	6338.0
80	5152.5
81	3967.0
82	3967.0
83	3102.0
84	2237.0
85	1946.5
86	1656.0
87	1564.0
88	1472.0
89	1472.0
90	1110.5
91	749.0
92	424.5
93	100.0
94	63.5
95	27.0
96	27.0
97	17.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2377543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.4649877062153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.35896146053827	40.51562056339754
2	8.43618434975126	8.008467728966187
3	2.2831081458677325	3.2510310022671574
4	0.9861324836973443	1.8722706486157599
5	0.5938526554575344	1.409360449529759
6	0.39139174972817725	1.1146442753497214
7	0.26616611362697945	0.8843499917780977
8	0.19923914988762811	0.7565507040010444
9	0.14289770234666882	0.6104373916617942
>10	1.0069156178822485	9.358155035810455
>50	0.1417941820123655	4.768214347885809
>100	0.16917859861321563	17.250476737060996
>500	0.018044454007007573	5.729807587974856
>1k	0.006044447647667562	4.234427631709353
>5k	8.88889359951112E-5	0.2361859039916001
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5598	0.2354531547904707	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4676	0.19667362483033954	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4404	0.18523324288982365	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3099	0.13034464571198082	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2524	0.10616001477155197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.412045544496987E-5	0.0
2	0.0	0.0	0.0	5.888431881147891E-4	0.0
3	0.0	0.0	0.0	0.001303867059397033	0.0
4	0.0	0.0	0.0	0.0022291920692917013	0.0
5	0.0	0.0	0.0	0.0024815534356266112	0.0
6	0.0	0.0	0.0	0.002523613663349096	0.0
7	0.0	0.0	0.0	0.0028180352574064906	0.0
8	0.0	0.0	0.0	0.0029021557128514606	0.0
9	0.0	0.0	0.0	0.0048789864158082525	0.0
10	0.0	0.0	0.0	0.007150238712822439	0.0
11	0.0	0.0	0.0	0.008285864861329532	0.0
12	0.0	0.0	0.0	0.009085009188056745	0.0
13	0.0	0.0	0.0	0.009589731920726565	0.0
14	0.0	0.0	0.0	0.01030475579200881	0.0
15	0.0	0.0	0.0	0.011398321712793417	0.0
16	0.0	0.0	0.0	0.012660128544467965	0.0
17	0.0	0.0	0.0	0.01371163423753009	0.0
18	0.0	0.0	0.0	0.014679019475147242	0.0
19	0.0	0.0	0.0	0.015898766079099305	0.0
20	0.0	0.0	0.0	0.017454994504831248	0.0
21	0.0	0.0	0.0	0.01943182520778804	0.0
22	0.0	0.0	0.0	0.022081619554304592	0.0
23	0.0	4.206022772248493E-5	0.0	0.026287642326553083	0.0
24	0.0	4.206022772248493E-5	0.0	0.03318551967304061	0.0
25	0.0	4.206022772248493E-5	0.0	0.03596149470272462	0.0
26	0.0	4.206022772248493E-5	0.0	0.041134902712590266	0.0
27	0.0	4.206022772248493E-5	0.0	0.053416489207555866	0.0
28	0.0	4.206022772248493E-5	0.0	0.07957795085094149	0.0
29	0.0	4.206022772248493E-5	0.0	0.1203343115140294	0.0
30	0.0	4.206022772248493E-5	0.0	0.17741004053344145	0.0
31	0.0	4.206022772248493E-5	0.0	0.37736436312613486	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	25	0.0054975096	29.6	6
GGTATCA	2625	0.0	20.438095	1
CGTCTAT	55	5.145316E-4	20.181818	1
AAGACGG	550	0.0	19.172728	5
CAAGACG	575	0.0	18.017391	4
CGCAAGA	620	0.0	17.008064	2
GCGCAAG	635	0.0	16.023623	1
ACGGACC	605	0.0	15.900826	8
ACGCTTC	290	0.0	15.310345	31
AGTACCG	85	5.367144E-4	15.235295	5
GACGGAC	645	0.0	14.914729	7
TTAGAAC	150	8.112147E-8	14.8	3
TACCGTG	75	0.004106122	14.8	7
GAGCGAA	660	0.0	14.575757	16
AACGATT	205	9.276846E-11	14.439025	22
CGATTAA	180	3.3360266E-9	14.388888	24
GCTTTAT	695	0.0	14.374101	1
GAGTACG	595	0.0	14.302521	1
CGGACCA	690	0.0	14.210144	9
CTAGCAC	430	0.0	14.197676	3
>>END_MODULE